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Tgfb1 (基因名), Transforming growth factor beta-1 proprotein (蛋白名), tgfb1_rat.

产品名称:

Rat Tgfb1/ Transforming growth factor beta-1 proprotein ELISA Kit
转化生长因子β-1

货号:

E0124r

商标:

EIAab^®

监管等级:

RUO

检测方法:

ELISA

实验类型:

Sandwich

检测范围:

15.6-1000pg/mL

灵敏度:

7.82pg/mL

特异性:

Natural and recombinant rat Transforming growth factor beta-1 proprotein

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

实验步骤:

研究领域:

Cancer

规格 & 价格:

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Rat Tgfb1 ELISA Kit

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产品说明书

数据表:

说明书:

MSDS:

在线询价

数据
文献 ( 7 )
序列 / 3D
常见问题解答
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精密度

批内差：已知浓度的3个样本在一个板子内重复检测20次，以评估批内精密度。
批内 CV： ≤5.2%
批间差：已知浓度的3个样本在不同的板子上重复测定5次，以评估测定批间精密度。
批间 CV： ≤7.4%

回收率

回收率：低、中和高浓度的分析物被掺入到血清或者血浆样本中，进行回收实验测定。

Sample Type	Average(%)	Recovery Range(%)
Serum	101	95-107
Plasma	103	97-109

线性

线性：给定样本通过梯度稀释，每次稀释的测量值与理论值的比值。

Sample	1：2	1:4	1:8	1:16
serum(n=5)	94-104%	88-98%	92-102%	86-94%
EDTA plasma(n=5)	101-110%	104-112%	93-102%	101-110%
heparin plasma(n=5)	105-114%	91-101%	108-117%	108-118%

通用注释

亚单元:

Homodimer; disulfide-linked (By similarity). Interacts with the serine proteases, HTRA1 and HTRA3: the interaction with either inhibits TGFB1-mediated signaling. The HTRA protease activity is required for this inhibition. May interact with THSD4; this interaction may lead to sequestration by FBN1 microfibril assembly and attenuation of TGFB signaling (By similarity). Interacts with CD109, DPT and ASPN. Latency-associated peptide: Homodimer; disulfide-linked. Latency-associated peptide: Interacts with Transforming growth factor beta-1 (TGF-beta-1) chain; interaction is non-covalent and maintains (TGF-beta-1) in a latent state; each Latency-associated peptide (LAP) monomer interacts with TGF-beta-1 in the other monomer. Latency-associated peptide: Interacts with LTBP1; leading to regulate activation of TGF-beta-1. Latency-associated peptide: Interacts with LRRC32/GARP; leading to regulate activation of TGF-beta-1 on the surface of activated regulatory T-cells (Tregs). Interacts with LRRC33/NRROS; leading to regulate activation of TGF-beta-1 in macrophages and microglia. Latency-associated peptide: Interacts (via cell attachment site) with integrins ITGAV and ITGB6 (ITGAV:ITGB6), leading to release of the active TGF-beta-1 (By similarity). Latency-associated peptide: Interacts with NREP; the interaction results in a decrease in TGFB1 autoinduction (By similarity). Latency-associated peptide: Interacts with HSP90AB1; inhibits latent TGFB1 activation. Transforming growth factor beta-1: Homodimer; disulfide-linked. Transforming growth factor beta-1: Interacts with TGF-beta receptors (TGFBR1 and TGFBR2), leading to signal transduction (By similarity).

功能:

Transforming growth factor beta-1: Multifunctional protein that regulates the growth and differentiation of various cell types and is involved in various processes, such as normal development, immune function, microglia function and responses to neurodegeneration (By similarity). Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-1 (TGF-beta-1) chains remain non-covalently linked rendering TGF-beta-1 inactive during storage in extracellular matrix (By similarity). At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1, LRRC32/GARP and LRRC33/NRROS that control activation of TGF-beta-1 and maintain it in a latent state during storage in extracellular milieus. TGF-beta-1 is released from LAP by integrins (ITGAV:ITGB6 or ITGAV:ITGB8): integrin-binding to LAP stabilizes an alternative conformation of the LAP bowtie tail and results in distortion of the LAP chain and subsequent release of the active TGF-beta-1 (By similarity). Once activated following release of LAP, TGF-beta-1 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal (By similarity). While expressed by many cells types, TGF-beta-1 only has a very localized range of action within cell environment thanks to fine regulation of its activation by Latency-associated peptide chain (LAP) and 'milieu molecules' (PubMed:2070682). Plays an important role in bone remodeling: acts as a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts (PubMed:2070682). Can promote either T-helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner (By similarity). At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development (By similarity). At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells (By similarity). Stimulates sustained production of collagen through the activation of CREB3L1 by regulated intramembrane proteolysis (RIP). Mediates SMAD2/3 activation by inducing its phosphorylation and subsequent translocation to the nucleus. Can induce epithelial-to-mesenchymal transition (EMT) and cell migration in various cell types (By similarity).

亚细胞位置:

Transforming growth factor beta-1 Secreted

数据库链接

UniGene:

Rn.40136

SMR:

P17246

STRING:

10116.ENSRNOP00000028051

KEGG:

rno:59086

Pfam:

PF00019

Uniprot:

P17246

相关产品

Human TGFB1 ELISA Kit

Mouse Tgfb1 ELISA Kit

Bovine TGFB1 ELISA Kit

Chicken TGFB1 ELISA Kit

Pig TGFB1 ELISA Kit

序列长度:

390

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

250:

300:

350:

396:

HNPGASASPC | CVPQA LEPL | PIVYYVGRKP | KVEQLSNMIV | RSCKCS

3D 模型:

3D 结构:

免疫原序列:

验证序列:

[1].

"Berberine attenuates podocytes injury caused by exosomes derived from high glucose-induced mesangial cells through TGFβ1-PI3K/AKT pathway"

Y Wang, LQ Tang, W Wei.

From: European Journal of Pharmacology

[2].

"Probiotic-prebiotic-synbiotic modulation of (YAP1, LATS1 and NF2 mRNAs/miR-1205/lncRNA SRD5A3-AS2) panel in NASH animal model"

S H.Gadallah, S Eissa, H M. Ghanem, et al.

From: Biomedicine & Pharmacotherapy

[3].

"Protective effects of Brownea grandiceps (Jacq.) against ϒ-radiation-induced enteritis in rats in relation to its secondary metabolome fingerprint"

D A. Korany, R S. Said, I M. Ayoub, et al.

From: Biomedicine & Pharmacotherapy

[4].

"Coenzyme Q10 attenuates inflammation and fibrosis implicated in radiation enteropathy through suppression of NF-kB/TGF-β/MMP-9 pathways"

H A. Mohamed, R S. Said.

From: International Immunopharmacology

[5].

"Modulation of hepatic stellate cells by Mutaflor® probiotic in non-alcoholic fatty liver disease management"

N M. Hany, S Eissa, M Basyouni, et al.

From: Journal of Translational Medicine

[6].

"Simvastatin Attenuates Intestinal Fibrosis Independent of the Anti-Inflammatory Effect by Promoting Fibroblast/Myofibroblast Apoptosis"

Y Abe, M Murano, N Murano, et al.

From: Digestive diseases and sciences

[7].

"Tanshinone IIA Attenuates Peritoneal Fibrosis through Inhibition of Fibrogenic Growth Factors Expression in Peritoneum in a Peritoneal Dialysis Rat"

Jiang Chunming, Zhang Miao, Sun Cheng, Tao Nana, Zhu Wei, Cao Dongwei and Feng Yuan

From: Informa healthcare

[1].

大鼠转化生长因子β-1(Tgfb1)ELISA试剂盒可以做多少个样本？

大鼠转化生长因子β-1(Tgfb1)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

大鼠转化生长因子β-1(Tgfb1)ELISA试剂盒使用视频？

大鼠转化生长因子β-1(Tgfb1)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

大鼠转化生长因子β-1(Tgfb1)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的大鼠转化生长因子β-1(Tgfb1)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

大鼠转化生长因子β-1(Tgfb1)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

大鼠转化生长因子β-1(Tgfb1)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析大鼠转化生长因子β-1(Tgfb1)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

大鼠转化生长因子β-1(Tgfb1)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集大鼠转化生长因子β-1(Tgfb1)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

大鼠转化生长因子β-1(Tgfb1)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

大鼠转化生长因子β-1(Tgfb1)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

大鼠转化生长因子β-1(Tgfb1)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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Rat Tgfb1/ Transforming growth factor beta-1 proprotein ELISA Kit 转化生长因子β-1

E0124r

Rat Tgfb1/ Transforming growth factor beta-1 proprotein ELISA Kit
转化生长因子β-1