首页 > 重组蛋白 > Human EFNA1 Recombinant Protein

EFNA1 (基因名), Ephrin-A1 (蛋白名), efna1_human.

产品名称:

Human EFNA1/ Ephrin-A1 Recombinant Protein
肝配蛋白A1

货号:

R1422h

商标:

EIAab^®

监管等级:

RUO

别名:

EPH-related receptor tyrosine kinase ligand 1, Immediate early response protein B61, Tumor necrosis factor alpha-induced protein 4, LERK-1, TNF alpha-induced protein 4, EPLG1, LERK1, TNFAIP4

序列号:

P20827

来源:

E.coli

种属:

Human

标签:

His

序列:

31-170aa

预估分子量:

15.4 kDa (monomer)

纯度:

Greater than 90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Cardiovascular

规格 & 价格:

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产品说明书

说明书:

MSDS:

在线询价

数据
文献 ( 1 )
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

Recombinant human EFNA1 protein was determined by 12% SDS-PAGE stained with Coomassie Blue under reducing conditions.

基因位点

Cytogenetic band: 1q22 by HGNC 1q22 by Entrez Gene 1q22 by Ensembl

EFNA1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Monomer. Homodimer. Forms heterodimers with EPHA2. Binds to the receptor tyrosine kinases EPHA2, EPHA3, EPHA4, EPHA5, EPHA6 and EPHA7. Also binds with low affinity to EPHA1.

功能:

Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. Plays an important role in angiogenesis and tumor neovascularization. The recruitment of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. Exerts anti-oncogenic effects in tumor cells through activation and down-regulation of EPHA2. Activates EPHA2 by inducing tyrosine phosphorylation which leads to its internalization and degradation. Acts as a negative regulator in the tumorigenesis of gliomas by down-regulating EPHA2 and FAK. Can evoke collapse of embryonic neuronal growth cone and regulates dendritic spine morphogenesis.

亚细胞位置:

Ephrin-A1, secreted form Secreted

数据库链接

UniGene:

Hs.516664

SMR:

P20827

STRING:

9606.ENSP00000357392

KEGG:

hsa:1942

MIM:

191164

Pfam:

PF00812

Uniprot:

P20827

相关产品

Mouse Efna1 ELISA Kit

Rat Efna1 ELISA Kit

Bovine EFNA1 ELISA Kit

Pig EFNA1 ELISA Kit

Mouse Efna1 CLIA Kit

序列长度:

205

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

205:

LLQTP

3D 模型:

3D 结构:

免疫原序列:

验证序列:

[1].

"Molecular targeting of malignant glioma cells with an EphA2-specific immunotoxin delivered by human bone marrow-derived mesenchymal stem cells"

X Sun, Z Xu, Y Ke, et al.

From: Cancer Letters

[1].

"Ligand recognition by A-class Eph receptors: crystal structures of the EphA2 ligand-binding domain and the EphA2/ephrin-A1 complex."

(PMID: 19525919)

[2].

"Ephrin-A1 is a negative regulator in glioma through down-regulation of EphA2 and FAK."

(PMID: 17332925)

[3].

"The genes encoding the eph-related receptor tyrosine kinase ligands LERK-1 (EPLG1, Epl1), LERK-3 (EPLG3, Epl3), and LERK-4 (EPLG4, Epl4) are clustered on human chromosome 1 and mouse chromosome 3."

(PMID: 8660976)

[4].

"A novel immediate-early response gene of endothelium is induced by cytokines and encodes a secreted protein."

(PMID: 2233719)

[5].

"Biological and structural characterization of glycosylation on ephrin-A1, a preferred ligand for EphA2 receptor tyrosine kinase."

(PMID: 23661698)

[6].

"A potential tumor suppressor role for Hic1 in breast cancer through transcriptional repression of ephrin-A1."

(PMID: 20154726)

[7].

"Architecture of Eph receptor clusters."

(PMID: 20505120)

[8].

"EphA2 mediates ligand-dependent inhibition and ligand-independent promotion of cell migration and invasion via a reciprocal regulatory loop with Akt."

(PMID: 19573808)

[9].

"Soluble monomeric EphrinA1 is released from tumor cells and is a functional ligand for the EphA2 receptor."

(PMID: 18794797)

[10].

"Green tea catechin inhibits ephrin-A1-mediated cell migration and angiogenesis of human umbilical vein endothelial cells."

(PMID: 17049832)

[1].

人肝配蛋白A1(EFNA1)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人肝配蛋白A1(EFNA1)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人肝配蛋白A1(EFNA1)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人肝配蛋白A1(EFNA1)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人肝配蛋白A1(EFNA1)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人肝配蛋白A1(EFNA1)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人肝配蛋白A1(EFNA1)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人肝配蛋白A1(EFNA1)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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