首页 > 重组蛋白 > Pig IL1B Recombinant Protein

IL1B (基因名), Interleukin-1 beta (蛋白名), il1b_pig.

产品名称:

Pig IL1B/ Interleukin-1 beta Recombinant Protein
Interleukin-1β

货号:

R0563p

商标:

EIAab^®

监管等级:

RUO

别名:

IL-1 beta

序列号:

P26889

来源:

E.coli

种属:

Pig

标签:

His

序列:

115-267aa

预估分子量:

16.83 kDa (monomer)

纯度:

Greater than 95% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Neurosciences

规格 & 价格:

关注

Pig IL1B Protein

邮箱 *

消息 *

Please 登录.

产品说明书

说明书:

MSDS:

在线询价

数据
文献 ( 2 )
序列 / 3D
常见问题解答
反馈墙 (0)

R&D 技术数据

The PCR product of pig IL1B gene was determined by 1% Agarose stained with EB.

Recombinant pig IL1B protein was determined by 12% SDS-PAGE stained with Coomassie Blue under reducing conditions.

通用注释

亚单元:

Monomer. Interacts with MEFV.

功能:

Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production.

亚细胞位置:

Cytoplasm Cytosol Lysosome Secreted Exosome Cytoplasmic vesicle Autophagosome Secreted The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood. On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca(2+)-dependent exocytosis with release of mature IL1B. 2. Secretory autophagy: IL1B-containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes. However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial. 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes. 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis. 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome. These mechanisms may not be not mutually exclusive.

数据库链接

UniGene:

Ssc.28829

SMR:

P26889

STRING:

9823.ENSSSCP00000008636

KEGG:

ssc:397122

Pfam:

PF00340

Uniprot:

P26889

相关产品

Human IL1B ELISA Kit

Mouse Il1b ELISA Kit

Rat Il1b ELISA Kit

Bovine IL1B ELISA Kit

Dog IL1B ELISA Kit

序列长度:

267

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

250:

267:

SKGRQDITDF | TMEVLSP

3D 模型:

3D 结构:

免疫原序列:

验证序列:

[1].

"PPARγ ligands modulate the immune response mediators in the pig myometrium — an in vitro study"

A Kurzyńska, Z Kunicka, K Mierzejewski, et al.

From: Animal Reproduction Science

[2].

"Peroxisome proliferator-activated receptor gamma ligands regulate the expression of inflammatory mediators in porcine endometrium during LPS-induced inflammation"

K Mierzejewski, A Kurzyńska, Z Kunicka, et al.

From: Theriogenology

[1].

猪Interleukin-1β(IL1B)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

猪Interleukin-1β(IL1B)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定猪Interleukin-1β(IL1B)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

猪Interleukin-1β(IL1B)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

猪Interleukin-1β(IL1B)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

猪Interleukin-1β(IL1B)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

猪Interleukin-1β(IL1B)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

猪Interleukin-1β(IL1B)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

反馈墙

评论数 : 0

所有用户