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ATF4 (基因名), Cyclic AMP-dependent transcription factor ATF-4 (蛋白名), atf4_bovin.

产品名称:

Bovine ATF4/ Cyclic AMP-dependent transcription factor ATF-4 ELISA Kit
cAMP-dependenttranscriptionfactorATF-4

货号:

E9187b

商标:

EIAab^®

监管等级:

RUO

别名:

Activating transcription factor 4, cAMP-dependent transcription factor ATF-4

检测方法:

ELISA

特异性:

Natural and recombinant bovine Cyclic AMP-dependent transcription factor ATF-4

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

研究领域:

Cancer

规格 & 价格:

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产品说明书

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数据
文献
序列 / 3D
常见问题解答
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通用注释

亚单元:

Binds DNA as a homodimer and as a heterodimer. Interacts with CEBPB and binds DNA as a heterodimer with CEBPB. Interacts with NEK6, DAPK2 and ZIPK/DAPK3. Interacts (via its leucine zipper domain) with GABBR1 and GABBR2 (via their C-termini). Forms a heterodimer with TXLNG in osteoblasts. Interacts (via its DNA binding domain) with FOXO1 (C-terminal half); the interaction occurs in osteoblasts and regulates glucose homeostasis through suppression of beta-cell proliferation and a decrease in insulin production. Interacts with SATB2; the interaction results in enhanced DNA binding and transactivation by these transcription factors (By similarity). Interacts with CEP290 (via an N-terminal region) (PubMed:16682973). Interacts with DDIT3/CHOP.

功能:

Transcriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Cooperates with FOXO1 in osteoblasts to regulate glucose homeostasis through suppression of beta-cell production and decrease in insulin production. Regulates the induction of DDIT3/CHOP and asparagine synthetase (ASNS) in response to endoplasmic reticulum (ER) stress. In concert with DDIT3/CHOP, activates the transcription of TRIB3 and promotes ER stress-induced neuronal apoptosis by regulating the transcriptional induction of BBC3/PUMA. Activates transcription of SIRT4. Regulates the circadian expression of the core clock component PER2 and the serotonin transporter SLC6A4. Binds in a circadian time-dependent manner to the cAMP response elements (CRE) in the SLC6A4 and PER2 promoters and periodically activates the transcription of these genes. During ER stress response, activates the transcription of NLRP1, possibly in concert with other factors.

亚细胞位置:

Cytoplasm Cell membrane Nucleus Cytoplasm Cytoskeleton Microtubule organizing center Centrosome Colocalizes with GABBR1 in hippocampal neuron dendritic membranes. Colocalizes with NEK6 in the centrosome (By similarity).

数据库链接

UniGene:

Bt.49442

SMR:

Q3ZCH6

STRING:

9913.ENSBTAP00000023213

KEGG:

bta:509107

Pfam:

PF00170

Uniprot:

Q3ZCH6

相关产品

序列长度:

348

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

250:

300:

348:

EALTGECKEL | EKKNEALKEK | ADSLAKEIQY | LKDQIEEVRK | AREKKRVL

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

牛cAMP-dependenttranscriptionfactorATF-4(ATF4)ELISA试剂盒可以做多少个样本？

牛cAMP-dependenttranscriptionfactorATF-4(ATF4)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

牛cAMP-dependenttranscriptionfactorATF-4(ATF4)ELISA试剂盒使用视频？

牛cAMP-dependenttranscriptionfactorATF-4(ATF4)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

牛cAMP-dependenttranscriptionfactorATF-4(ATF4)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的牛cAMP-dependenttranscriptionfactorATF-4(ATF4)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

牛cAMP-dependenttranscriptionfactorATF-4(ATF4)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

牛cAMP-dependenttranscriptionfactorATF-4(ATF4)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析牛cAMP-dependenttranscriptionfactorATF-4(ATF4)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

牛cAMP-dependenttranscriptionfactorATF-4(ATF4)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集牛cAMP-dependenttranscriptionfactorATF-4(ATF4)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

牛cAMP-dependenttranscriptionfactorATF-4(ATF4)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

牛cAMP-dependenttranscriptionfactorATF-4(ATF4)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

牛cAMP-dependenttranscriptionfactorATF-4(ATF4)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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Bovine ATF4/ Cyclic AMP-dependent transcription factor ATF-4 ELISA Kit cAMP-dependenttranscriptionfactorATF-4

E9187b

Activating transcription factor 4, cAMP-dependent transcription factor ATF-4

Bovine ATF4/ Cyclic AMP-dependent transcription factor ATF-4 ELISA Kit
cAMP-dependenttranscriptionfactorATF-4