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Cacna1a (基因名), Voltage-dependent P/Q-type calcium channel subunit alpha-1A (蛋白名), cac1a_rat.
产品名称:

Rat Cacna1a/ Voltage-dependent P/Q-type calcium channel subunit alpha-1A ELISA Kit
电压依赖性P/Q型钙离子通道亚基α-1A

货号:

E1344r

商标:
EIAab®
监管等级:
别名:

Brain calcium channel I, Calcium channel, L type, alpha-1 polypeptide, isoform 4, Rat brain class A, Voltage-gated calcium channel subunit alpha Cav2.1, BI, RBA-I, Cach4, Cacn3, Cacnl1a4

检测方法:
ELISA
实验类型:
Sandwich
检测范围:
0.312-20ng/mL
灵敏度:
0.174ng/mL
特异性:
Natural and recombinant rat Voltage-dependent P/Q-type calcium channel subunit alpha-1A
样品类型:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
样品数据:
实验步骤:
实验步骤
研究领域:
-
Rat Cacna1a ELISA Kit
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产品说明书
说明书: 下载说明书
MSDS: MSDS
在线询价


精密度
批内差:已知浓度的3个样本在一个板子内重复检测20次,以评估批内精密度。
批内 CV: ≤4.9%
批间差:已知浓度的3个样本在不同的板子上重复测定5次,以评估测定批间精密度。
批间 CV: ≤7.6%
回收率
回收率:低、中和高浓度的分析物被掺入到血清或者血浆样本中,进行回收实验测定。

Sample Type

Average(%)

Recovery Range(%)

Serum

95

89-101

Plasma

97

91-103

 

 

 

 

线性
线性:给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。

Sample

1:2

1:4

1:8

1:16

serum(n=5)

89-99%

89-100%

83-93%

100-109%

EDTA plasma(n=5)

105-114%

103-113%

109-121%

97-107%

heparin plasma(n=5)

118-127%

 

80-90%

86-96%

90-102%

 

通用注释


亚单元:
Voltage-dependent calcium channels are multisubunit complexes, consisting of alpha-1, alpha-2, beta and delta subunits in a 1:1:1:1 ratio. The channel activity is directed by the pore-forming and voltage-sensitive alpha-1 subunit. In many cases, this subunit is sufficient to generate voltage-sensitive calcium channel activity. The auxiliary subunits beta and alpha-2/delta linked by a disulfide bridge regulate the channel activity. Interact (via C-terminal CDB motif) with CABP1 in the pre- and postsynaptic membranes.


功能:
Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1A gives rise to P and/or Q-type calcium currents. P/Q-type calcium channels belong to the 'high-voltage activated' (HVA) group and are blocked by the funnel toxin (Ftx) and by the omega-agatoxin-IVA (omega-Aga-IVA). They are however insensitive to dihydropyridines (DHP), and omega-conotoxin-GVIA (omega-CTx-GVIA).


亚细胞位置:
Cell membrane Multi-pass membrane protein


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