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EPHA4 (基因名), Ephrin type-A receptor 4 (蛋白名), epha4_human.

产品名称:

Human EPHA4/ Ephrin type-A receptor 4 ELISA Kit
A型肝配蛋白受体4

货号:

E0303h

商标:

EIAab^®

监管等级:

RUO

别名:

EPH-like kinase 8, Tyrosine-protein kinase TYRO1, Tyrosine-protein kinase receptor SEK, EK8, HEK8, SEK, TYRO1

检测方法:

ELISA

实验类型:

Sandwich

检测范围:

15.6-1000U/L

灵敏度:

10U/L

特异性:

Natural and recombinant human Ephrin type-A receptor 4

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

实验步骤:

研究领域:

Signal Transduction

规格 & 价格:

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产品说明书

数据表:

说明书:

MSDS:

在线询价

数据
文献
序列 / 3D
常见问题解答
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精密度

批内差：已知浓度的3个样本在一个板子内重复检测20次，以评估批内精密度。
批内 CV： ≤4.5%
批间差：已知浓度的3个样本在不同的板子上重复测定5次，以评估测定批间精密度。
批间 CV： ≤9.8%

回收率

回收率：低、中和高浓度的分析物被掺入到血清或者血浆样本中，进行回收实验测定。

Sample Type	Average(%)	Recovery Range(%)
Serum	101	95-107
Plasma	103	97-109

线性

线性：给定样本通过梯度稀释，每次稀释的测量值与理论值的比值。

Sample	1：2	1:4	1:8	1:16
serum(n=5)	89-98%	101-113%	97-108%	102-113%
EDTA plasma(n=5)	103-112%	98-108%	89-98%	109-119%
heparin plasma(n=5)	88-98%	82-92%	98-109%	91-100%

通用注释

亚单元:

Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses. Interacts (phosphorylated at position Tyr-602) with FYN. Interacts with CDK5, CDK5R1 and NGEF; upon activation by EFNA1 induces NGEF phosphorylation by the kinase CDK5. Interacts with CHN1; effector of EPHA4 in axon guidance linking EPHA4 activation to RAC1 regulation (By similarity). Interacts (via PDZ motif) with SIPA1L1 (via PDZ domain); controls neuronal morphology through regulation of the RAP1 (RAP1A or RAP1B) and RAP2 (RAP2A, RAP2B or RAP2C) GTPases.

功能:

Receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous, it has the unique property among Eph receptors to bind and to be physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands including EFNA1 and EFNB3. Upon activation by ephrin ligands, modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, Rap and Rho GTPases activity. Plays an important role in the development of the nervous system controlling different steps of axonal guidance including the establishment of the corticospinal projections. May also control the segregation of motor and sensory axons during neuromuscular circuit development. In addition to its role in axonal guidance plays a role in synaptic plasticity. Activated by EFNA1 phosphorylates CDK5 at 'Tyr-15' which in turn phosphorylates NGEF regulating RHOA and dendritic spine morphogenesis. In the nervous system, plays also a role in repair after injury preventing axonal regeneration and in angiogenesis playing a role in central nervous system vascular formation. Additionally, its promiscuity makes it available to participate in a variety of cell-cell signaling regulating for instance the development of the thymic epithelium.

亚细胞位置:

Cell membrane Single-pass type I membrane protein Cell projection Axon Cell projection Dendrite Cell junction Synapse Postsynaptic cell membrane Postsynaptic density Early endosome Clustered upon activation and targeted to early endosome.

数据库链接

UniGene:

Hs.371218

SMR:

P54764

STRING:

9606.ENSP00000281821

KEGG:

hsa:2043

MIM:

602188

Pfam:

PF14575

Uniprot:

P54764

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Mouse Epha4 CLIA Kit

Chicken EPHA4 CLIA Kit

Mouse Epha4 Protein

序列长度:

986

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

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850:

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950:

986:

LARIGITAIT | HQNKILSSVQ | AMRTQMQQMH | GRMVPV

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

人A型肝配蛋白受体4(EPHA4)ELISA试剂盒可以做多少个样本？

人A型肝配蛋白受体4(EPHA4)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

人A型肝配蛋白受体4(EPHA4)ELISA试剂盒使用视频？

人A型肝配蛋白受体4(EPHA4)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

人A型肝配蛋白受体4(EPHA4)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的人A型肝配蛋白受体4(EPHA4)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

人A型肝配蛋白受体4(EPHA4)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

人A型肝配蛋白受体4(EPHA4)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析人A型肝配蛋白受体4(EPHA4)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

人A型肝配蛋白受体4(EPHA4)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集人A型肝配蛋白受体4(EPHA4)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

人A型肝配蛋白受体4(EPHA4)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

人A型肝配蛋白受体4(EPHA4)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

人A型肝配蛋白受体4(EPHA4)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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Human EPHA4/ Ephrin type-A receptor 4 ELISA Kit A型肝配蛋白受体4

E0303h

EPH-like kinase 8, Tyrosine-protein kinase TYRO1, Tyrosine-protein kinase receptor SEK, EK8, HEK8, SEK, TYRO1

Human EPHA4/ Ephrin type-A receptor 4 ELISA Kit
A型肝配蛋白受体4