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Gapdh (基因名), Glyceraldehyde-3-phosphate dehydrogenase (蛋白名), g3p_rat.

产品名称:

Rat Gapdh/ Glyceraldehyde-3-phosphate dehydrogenase ELISA Kit
3-磷酸甘油醛脱氢

货号:

E1932r

商标:

EIAab^®

监管等级:

RUO

别名:

38 kDa BFA-dependent ADP-ribosylation substrate, BARS-38, Peptidyl-cysteine S-nitrosylase GAPDH, GAPDH, Gapd

检测方法:

ELISA

实验类型:

Sandwich

检测范围:

0.156-10ng/mL

灵敏度:

0.065ng/mL

特异性:

Natural and recombinant rat Glyceraldehyde-3-phosphate dehydrogenase

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

实验步骤:

研究领域:

Neurosciences

规格 & 价格:

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产品说明书

数据表:

说明书:

MSDS:

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数据
文献
序列 / 3D
常见问题解答
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通用注释

亚单元:

Homotetramer. Interacts with EIF1AD, USP25, PRKCI and WARS. Interacts with TPPP; the interaction is direct (By similarity). Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation. Interacts with CHP1; the interaction increases the binding of CHP1 with microtubules. Interacts with phosphorylated RPL13A (By similarity). Associates with microtubules. Component of the GAIT complex. Interacts with FKBP6; leading to inhibit GAPDH catalytic activity.

功能:

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.

亚细胞位置:

Cytoplasm Cytosol Cytoplasm Cytoskeleton Nucleus Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal. Colocalizes with CHP1 to small punctate structures along the microtubules tracks.

数据库链接

UniGene:

Rn.129558

SMR:

P04797

STRING:

10116.ENSRNOP00000040878

KEGG:

rno:24383

Pfam:

PF02800

Uniprot:

P04797

相关产品

Human GAPDH ELISA Kit

Mouse Gapdh ELISA Kit

Bovine GAPDH ELISA Kit

Chicken GAPDH ELISA Kit

Pig GAPDH ELISA Kit

序列长度:

333

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

250:

300:

333:

LNDNFVKLIS | WYDNEYGYSN | RVVDLMAYMA | SKE

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

大鼠3-磷酸甘油醛脱氢(Gapdh)ELISA试剂盒可以做多少个样本？

大鼠3-磷酸甘油醛脱氢(Gapdh)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

大鼠3-磷酸甘油醛脱氢(Gapdh)ELISA试剂盒使用视频？

大鼠3-磷酸甘油醛脱氢(Gapdh)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

大鼠3-磷酸甘油醛脱氢(Gapdh)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的大鼠3-磷酸甘油醛脱氢(Gapdh)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

大鼠3-磷酸甘油醛脱氢(Gapdh)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

大鼠3-磷酸甘油醛脱氢(Gapdh)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析大鼠3-磷酸甘油醛脱氢(Gapdh)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

大鼠3-磷酸甘油醛脱氢(Gapdh)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集大鼠3-磷酸甘油醛脱氢(Gapdh)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

大鼠3-磷酸甘油醛脱氢(Gapdh)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

大鼠3-磷酸甘油醛脱氢(Gapdh)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

大鼠3-磷酸甘油醛脱氢(Gapdh)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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Rat Gapdh/ Glyceraldehyde-3-phosphate dehydrogenase ELISA Kit 3-磷酸甘油醛脱氢

E1932r

38 kDa BFA-dependent ADP-ribosylation substrate, BARS-38, Peptidyl-cysteine S-nitrosylase GAPDH, GAPDH, Gapd

Rat Gapdh/ Glyceraldehyde-3-phosphate dehydrogenase ELISA Kit
3-磷酸甘油醛脱氢