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Rpe65 (基因名), Retinoid isomerohydrolase (蛋白名), rpe65_rat.
产品名称:

Rat Rpe65/ Retinoid isomerohydrolase ELISA Kit
RPE65

货号:

E9829r

商标:
EIAab®
监管等级:
别名:

All-trans-retinyl-palmitate hydrolase, Lutein isomerase, Meso-zeaxanthin isomerase, Retinal pigment epithelium-specific 65 kDa protein, Retinol isomerase

检测方法:
ELISA
实验类型:
Sandwich
检测范围:
78-5000pg/mL
灵敏度:
39pg/mL
特异性:
Natural and recombinant rat Retinoid isomerohydrolase
样品类型:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
样品数据:
实验步骤:
实验步骤
研究领域:
-
Rat Rpe65 ELISA Kit
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产品说明书
说明书: 下载说明书
MSDS: MSDS
在线询价


精密度
批内差:已知浓度的3个样本在一个板子内重复检测20次,以评估批内精密度。
批内 CV: ≤5.4%
批间差:已知浓度的3个样本在不同的板子上重复测定5次,以评估测定批间精密度。
批间 CV: ≤10.4%
回收率
回收率:低、中和高浓度的分析物被掺入到血清或者血浆样本中,进行回收实验测定。

Sample Type

Average(%)

Recovery Range(%)

Serum

83

80-89

Plasma

85

80-91

 

 

 

 

线性
线性:给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。

Sample

1:2

1:4

1:8

1:16

serum(n=5)

95-105%

98-106%

110-120%

90-99%

EDTA plasma(n=5)

82-92%

101-110%

110-119%

101-112%

heparin plasma(n=5)

112-120%

 

106-114%

108-120%

104-114%

 

通用注释


亚单元:
Interacts with MYO7A; this mediates light-dependent intracellular transport of RPE65.


功能:
Critical isomerohydrolase in the retinoid cycle involved in regeneration of 11-cis-retinal, the chromophore of rod and cone opsins. Catalyzes the cleavage and isomerization of all-trans-retinyl fatty acid esters to 11-cis-retinol which is further oxidized by 11-cis retinol dehydrogenase to 11-cis-retinal for use as visual chromophore. Essential for the production of 11-cis retinal for both rod and cone photoreceptors. Also capable of catalyzing the isomerization of lutein to meso-zeaxanthin an eye-specific carotenoid. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT.


亚细胞位置:
Cytoplasm Cell membrane Lipid-anchor Microsome membrane Attached to the membrane by a lipid anchor when palmitoylated (membrane form), soluble when unpalmitoylated. Undergoes light-dependent intracellular transport to become more concentrated in the central region of the retina pigment epithelium cells (By similarity).


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