PCNA (基因名), Proliferating cell nuclear antigen (蛋白名), pcna_human.
产品名称:
PCNA Antibody
货号:
P0591Rb
商标:
EIAab®
监管等级:
别名:
Cyclin, PCNA
序列号:
P12004
反应性:
human, mouse, rat
免疫原:
corresponding to a sequence mapping at the 1-261aa of P12004
形态:
Liquid
存储说明:
4℃ for frequent use, -20℃ to -70℃ for 6 months
缓冲液:
0.1M×PBS,50% glycerol,pH7.5
浓度:
200ug/ml
纯化方式:
Immunogen affinity purified
克隆性:
Polyclonal
亚型:
Rabbit IgG
应用:
推荐产品:
研究领域:
Cancer
R&D 技术数据
293T lysate was subjected to SDS-PAGE followed by Western Blot with PCNA antibody at dilution of 1:300 (Observed molecular weight is about 30kDa)
通用注释
亚单元:
Homotrimer (PubMed:24939902). Interacts with p300/EP300; the interaction occurs on chromatin in UV-irradiated damaged cells (PubMed:24939902). Interacts with CREBBP (via transactivation domain and C-terminus); the interaction occurs on chromatin in UV-irradiated damaged cells (PubMed:24939902). Directly interacts with POLD1, POLD3 and POLD4 subunits of the DNA polymerase delta complex, POLD3 being the major interacting partner; the interaction with POLD3 is inhibited by CDKN1A/p21(CIP1) (PubMed:11595739, PubMed:16510448, PubMed:22148433, PubMed:24939902). Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with EXO1, POLH, POLK, DNMT1, ERCC5, FEN1, CDC6 and POLDIP2 (PubMed:9305916, PubMed:9302295, PubMed:9566895, PubMed:11784855, PubMed:12522211, PubMed:15225546, PubMed:15149598, PubMed:24911150, PubMed:15616578). Interacts with APEX2; this interaction is triggered by reactive oxygen species and increased by misincorporation of uracil in nuclear DNA (PubMed:11376153, PubMed:19443450). Forms a ternary complex with DNTTIP2 and core histone (PubMed:12786946). Interacts with KCTD10 and PPP1R15A (By similarity). Directly interacts with BAZ1B (PubMed:15543136). Interacts with HLTF and SHPRH (PubMed:17130289, PubMed:18316726, PubMed:18719106). Interacts with NUDT15; this interaction is disrupted in response to UV irradiation and acetylation (PubMed:19419956). Interacts with CDKN1A/p21(CIP1) and CDT1; interacts via their PIP-box which also recruits the DCX(DTL) complex. The interaction with CDKN1A inhibits POLD3 binding (PubMed:11595739, PubMed:16949367, PubMed:18794347, PubMed:18703516). Interacts with DDX11 (PubMed:18499658). Interacts with EGFR; positively regulates PCNA (PubMed:17115032). Interacts with PARPBP (PubMed:22153967). Interacts (when ubiquitinated) with SPRTN; leading to enhance RAD18-mediated PCNA ubiquitination (PubMed:22681887). Interacts (when polyubiquitinated) with ZRANB3 (PubMed:22704558, PubMed:22705370, PubMed:22759634). Interacts with SMARCAD1 (PubMed:21549307). Interacts with CDKN1C (PubMed:22634751). Interacts with PCLAF (via PIP-box) (PubMed:21628590, PubMed:23000965). Interacts with RTEL1 (via PIP-box); the interaction is direct and essential for the suppression of telomere fragility (PubMed:24115439). Interacts with FAM111A (via PIP-box); the interaction is direct and required for PCNA loading on chromatin binding (PubMed:24561620). Interacts with LIG1 (PubMed:24911150). Interacts with SETMAR (PubMed:20457750). Interacts with ANKRD17 (PubMed:23711367). Interacts with FBXO18/FBH1 (via PIP-box); the interaction recruits the DCX(DTL) complex and promotes ubiquitination and degradation of FBXO18/FBH1 (PubMed:23677613). Interacts with POLN (PubMed:19995904).
功能:
Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
亚细胞位置:
Nucleus
Colocalizes with CREBBP, EP300 and POLD1 to sites of DNA damage (PubMed:24939902). Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.
该产品尚未在任何出版物中被引用。
[1].
多克隆抗体PCNA可以申请产品的免费试用装吗?
我们不提供作测试目的的免费样品或试用装样品。如果某种抗体的性能与说明书上的数据说明不同,我们将提供换货或退款服务。如果抗体用于未测试的物种或应用,我们不提供换货或退款服务。EIAab产品的投诉期为6个月,逾期不予受理。
[2].
多克隆抗体PCNA说明书上的信息是及时更新的吗?
说明书包含了我们能获得的关于该产品的最新信息。当我们获得另外的信息时,说明书会实时更新,因此说明书绝不会过时。
[3].
如何选择合适的一抗?
请先确定您要检测的种属和使用的实验方法,然后查找相关产品,根据说明书上描述的“种属和应用”来确定合适的抗体。如果说明书中有明确您要检测的种属和实验方法均经过检测,则您可以放心地购买该产品。
[4].
多克隆抗体PCNA在其他物种或应用中测试过吗?
所有我们测试过抗体的物种和应用都会在说明书上说明。我们获取新信息后,就会立即更新物种和应用。
[5].
如何保存多克隆抗体PCNA?
我们建议始终按照说明书上的方法保存抗体。如果没有按照说明书上的说明保存,我们不能保证抗体的性能。
[6].
如何分装多克隆抗体PCNA?
请根据一次实验的常用量进行分装。每等份不应少于10μl;每等份的量越少,抗体储液浓度受蒸发和管壁吸附的影响越大。
反馈墙
评论数 : 0
所有用户
所有用户
默认排序
默认排序
最近
早期
目前还没有评论。
通知
规格
数量
单价 (¥)
小计 1 (¥)
小计 2:
¥
规格
数量
单价 (¥)