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RAG2 (基因名), V(D)J recombination-activating protein 2 (蛋白名), rag2_human.

产品名称:

RAG2 Antibody

货号:

P1551Rb

商标:

EIAab^®

监管等级:

RUO

别名:

RAG-2

序列号:

P55895

反应性:

human, mouse

免疫原:

corresponding to a sequence mapping at the Recombinant protein of P55895

形态:

Liquid

存储说明:

4℃ for frequent use, -20℃ to -70℃ for 6 months

缓冲液:

0.1M×PBS,50% glycerol,pH7.5

浓度:

200ug/ml

纯化方式:

Immunogen affinity purified

克隆性:

Polyclonal

亚型:

Rabbit IgG

应用:

推荐产品:

Goat Anti-Rabbit IgG(H+L) HRP-conjugated Antibody

研究领域:

规格 & 价格:

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Human RAG2 Polyclonal Antibody

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产品说明书

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R&D 技术数据

Immunohistochemical analysis of formalin-fixed paraffin embedded human tonsil tissue using RAG2 antibody.

通用注释

亚单元:

Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2.

功能:

Core component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. DNA cleavage by the RAG complex occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3.

亚细胞位置:

Nucleus

数据库链接

UniGene:

Hs.714519

SMR:

P55895

STRING:

9606.ENSP00000308620

KEGG:

hsa:5897

MIM: