首页 > 重组蛋白 > Human GDF5 Recombinant Protein

GDF5 (基因名), Growth/differentiation factor 5 (蛋白名), gdf5_human.

产品名称:

Human GDF5/ Growth/differentiation factor 5 Recombinant Protein
生长分化因子5

货号:

R0550h

商标:

EIAab^®

监管等级:

RUO

别名:

Bone morphogenetic protein 14, Cartilage-derived morphogenetic protein 1, Lipopolysaccharide-associated protein 4, Radotermin, BMP-14, CDMP-1, LAP-4, GDF-5, BMP14, CDMP1

序列号:

P43026

来源:

E.coli

种属:

Human

标签:

His

序列:

28-381aa

预估分子量:

38.94 kDa (monomer)

纯度:

>90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Cancer

规格 & 价格:

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产品说明书

说明书:

MSDS:

在线询价

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出版物
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常见问题解答
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R&D 技术数据

更多信息，请参阅手册，或联系我们的技术支持: tech@eiaab.com.

基因位点

Cytogenetic band: 20q11.22 by HGNC 20q11.22 by Entrez Gene 20q11.22 by Ensembl

GDF5 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Homodimer; disulfide-linked (By similarity). Interacts with serine proteases, HTRA1 and HTRA3 (By similarity). Following LPS binding, may form a complex with CXCR4, HSP90AA1 and HSPA8. Interacts with high affinity with NOG; inhibits chondrogenesis. Interacts with high affinity with BMPR1B and lower affinity with BMPR1A; positively regulates chondrocyte differentiation and induces SMAD dependent signaling. Interacts with FBN1 (via N-terminal domain) and FBN2 (PubMed:18339631).

功能:

Growth factor involved in bone and cartilage formation. During cartilage development regulates differentiation of chondrogenic tissue through two pathways. Firstly, positively regulates differentiation of chondrogenic tissue through its binding of high affinity with BMPR1B and of less affinity with BMPR1A, leading to induction of SMAD1-SMAD5-SMAD8 complex phosphorylation and then SMAD protein signaling transduction (PubMed:24098149, PubMed:21976273, PubMed:15530414, PubMed:25092592). Secondly, negatively regulates chondrogenic differentiation through its interaction with NOG (PubMed:21976273). Required to prevent excessive muscle loss upon denervation. This function requires SMAD4 and is mediated by phosphorylated SMAD1/5/8 (By similarity). Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes (PubMed:11276205).

亚细胞位置:

Secreted Cell membrane

数据库链接

UniGene:

Hs.1573

SMR:

P43026

STRING:

9606.ENSP00000363489

KEGG:

hsa:8200

MIM:

112600

Pfam:

PF00019

Uniprot:

P43026

相关产品

Mouse Gdf5 ELISA Kit

Mouse Gdf5 CLIA Kit

Mouse Gdf5 Protein

Mouse Gdf5 Polyclonal Antibody

Mouse Gdf5 Monoclonal Antibody

序列长度:

501

序列:

仅供参考，详细请咨询（services@eiaab.com）

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3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"A functional polymorphism in the 5' UTR of GDF5 is associated with susceptibility to osteoarthritis."

(PMID: 17384641)

[2].

"Mutations in GDF5 reveal a key residue mediating BMP inhibition by NOGGIN."

(PMID: 19956691)

[3].

"Crystal structure analysis reveals a spring-loaded latch as molecular mechanism for GDF-5-type I receptor specificity."

(PMID: 19229295)

[4].

"Genetic variation in the GDF5 region is associated with osteoarthritis, height, hip axis length and fracture risk: the Rotterdam study."

(PMID: 19029166)

[5].

"Association of the DVWA and GDF5 polymorphisms with osteoarthritis in UK populations."

(PMID: 19054821)

[6].

"Large-scale analysis of association between GDF5 and FRZB variants and osteoarthritis of the hip, knee, and hand."

(PMID: 19479880)

[7].

"Brachydactyly type A2 associated with a defect in proGDF5 processing."

(PMID: 18203755)

[8].

"Compound heterozygosity for GDF5 in Du Pan type chondrodysplasia."

(PMID: 18629880)

[9].

"Novel point mutations in GDF5 associated with two distinct limb malformations in Chinese: brachydactyly type C and proximal symphalangism."

(PMID: 18283415)

[10].

"The growth differentiation factor 5 (GDF5) core promoter polymorphism is not associated with knee osteoarthritis in the Greek population."

(PMID: 17676627)

[1].

人生长分化因子5(GDF5)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人生长分化因子5(GDF5)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人生长分化因子5(GDF5)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人生长分化因子5(GDF5)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人生长分化因子5(GDF5)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人生长分化因子5(GDF5)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人生长分化因子5(GDF5)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人生长分化因子5(GDF5)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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