首页 > 重组蛋白 > Human GPC3 Recombinant Protein

GPC3 (基因名), Glypican-3 (蛋白名), gpc3_human.

产品名称:

Human GPC3/ Glypican-3 Recombinant Protein
磷脂酰肌醇蛋白聚糖3

货号:

R0971h

商标:

EIAab^®

监管等级:

RUO

别名:

GTR2-2, Intestinal protein OCI-5, MXR7, OCI5

序列号:

P51654

来源:

E.coli

种属:

Human

标签:

His

序列:

25-206aa

预估分子量:

20.02 kDa (monomer)

纯度:

Greater than 98% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Cancer

规格 & 价格:

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Human GPC3 Protein

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产品说明书

说明书:

MSDS:

在线询价

数据
文献 ( 2 )
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

The sequence analysis of human GPC3 protein including hydrophilcity, hydrophobicity and antigenicity.

Recombinant human GPC3 protein was determined by 12% SDS-PAGE stained with Coomassie Blue under reducing conditions.

基因位点

Cytogenetic band: Xq26.2 by HGNC Xq26.2 by Entrez Gene Xq26.2 by Ensembl

GPC3 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Heterodimer; disulfide-linked (PubMed:14610063). Cleavage by a furin-like convertase results in production of alpha and beta chains which form a disulfide-linked heterodimer (PubMed:14610063). Interacts with DPP4 (PubMed:17549790). Interacts with FGF2 (By similarity). Interacts with WNT5A (PubMed:14610063). Also interacts with WNT3A and WNT7B (PubMed:16227623). Interacts with hedgehog protein SHH; the heparan sulfate chains are not required for the interaction (By similarity). Also interacts with hedgehog protein IHH (By similarity). Interacts with CD81 (By similarity). Interacts with Wnt receptors FZD4, FZD7 and FZD8; the heparan sulfate chains are required for the interaction (PubMed:24496449).

功能:

Cell surface proteoglycan that bears heparan sulfate (PubMed:14610063). Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins (By similarity). Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation (By similarity). Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands (PubMed:16227623, PubMed:24496449). Positively regulates the non-canonical Wnt signaling pathway (By similarity). Binds to CD81 which decreases the availability of free CD81 for binding to the transcriptional repressor HHEX, resulting in nuclear translocation of HHEX and transcriptional repression (By similarity). Inhibits the dipeptidyl peptidase activity of DPP4 (PubMed:17549790). Plays a role in limb patterning and skeletal development by controlling the cellular response to BMP4 (By similarity). Modulates the effects of growth factors BMP2, BMP7 and FGF7 on renal branching morphogenesis (By similarity). Required for coronary vascular development (By similarity). Plays a role in regulating cell movements during gastrulation (By similarity).

亚细胞位置:

Cell membrane Lipid-anchor GPI-anchor Extracellular side

数据库链接

UniGene:

Hs.644108

STRING:

9606.ENSP00000377836

KEGG:

hsa:2719

MIM:

300037

Pfam:

PF01153

Uniprot:

P51654

相关产品

序列长度:

580

序列:

仅供参考，详细请咨询（services@eiaab.com）

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589:

ATPKDNEIST | FHNLGNVHSP | LKLLTSMAIS | VVCFFFLVH

3D 模型:

3D 结构:

免疫原序列:

验证序列:

[1].

"Diagnostic and Prognostic Role of Serum Glypican 3 in Patients With Hepatocellular Carcinoma"

Hasan O¨ zkan, Harun Erdal, Erdem Koc-ak, Hu¨ seyin Tutkak, Zihni Karaeren,Mustafa Yakut, and Seyfettin Ko¨ klu¨

From: Journal of Clinical Laboratory Analysis

[2].

"GPC-HCC model: a combination of glybican-3 with other routine parameters improves the diagnostic efficacy in hepatocellular carcinoma"

Attallah A M, El-Far M, Omran M M, et al.

From: Tumor Biology

[1].

"Mutations in GPC3, a glypican gene, cause the Simpson-Golabi-Behmel overgrowth syndrome."

(PMID: 8589713)

[2].

"The Simpson-Golabi-Behmel syndrome causative glypican-3, binds to and inhibits the dipeptidyl peptidase activity of CD26."

(PMID: 17549790)

[3].

"Mutational analysis of the GPC3/GPC4 glypican gene cluster on Xq26 in patients with Simpson-Golabi-Behmel syndrome: identification of loss-of-function mutations in the GPC3 gene."

(PMID: 10814714)

[4].

"GPC4, the gene for human K-glypican, flanks GPC3 on xq26: deletion of the GPC3-GPC4 gene cluster in one family with Simpson-Golabi-Behmel syndrome."

(PMID: 9787072)

[5].

"Analysis of exon/intron structure and 400 kb of genomic sequence surrounding the 5'-promoter and 3'-terminal ends of the human glypican 3 (GPC3) gene."

(PMID: 9339360)

[1].

人磷脂酰肌醇蛋白聚糖3(GPC3)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人磷脂酰肌醇蛋白聚糖3(GPC3)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人磷脂酰肌醇蛋白聚糖3(GPC3)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人磷脂酰肌醇蛋白聚糖3(GPC3)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人磷脂酰肌醇蛋白聚糖3(GPC3)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人磷脂酰肌醇蛋白聚糖3(GPC3)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人磷脂酰肌醇蛋白聚糖3(GPC3)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人磷脂酰肌醇蛋白聚糖3(GPC3)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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