首页 > 重组蛋白 > Human H2AFX Recombinant Protein

H2AFX (基因名), Histone H2AX (蛋白名), h2ax_human.

产品名称:

Human H2AFX/ Histone H2AX Recombinant Protein
组蛋白H2A.x

货号:

R15010h

商标:

EIAab^®

监管等级:

RUO

别名:

Histone H2A.X, H2a/x, H2AX

序列号:

P16104

来源:

E.coli

种属:

Human

标签:

His

纯度:

>90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Epigenetics

规格 & 价格:

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Human H2AFX Protein

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产品说明书

说明书:

MSDS:

在线询价

数据
文献
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

更多信息，请参阅手册，或联系我们的技术支持: tech@eiaab.com.

基因位点

Cytogenetic band: 11q23.3 by HGNC 11q23.3 by Entrez Gene 11q23.3 by Ensembl

H2AFX Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with numerous proteins required for DNA damage signaling and repair when phosphorylated on Ser-140. These include MDC1, TP53BP1, BRCA1 and the MRN complex, composed of MRE11, RAD50, and NBN. Interaction with the MRN complex is mediated at least in part by NBN. Also interacts with DHX9/NDHII when phosphorylated on Ser-140 and MCPH1 when phosphorylated at Ser-140 or Tyr-143. Interacts with ARRB2; the interaction is detected in the nucleus upon OR1D2 stimulation. Interacts with Epstein-Barr virus protein EBNA6.

功能:

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

亚细胞位置:

Nucleus Chromosome

数据库链接

UniGene:

Hs.477879

SMR:

P16104

STRING:

9606.ENSP00000364310

KEGG:

hsa:3014

MIM:

601772

Pfam:

PF00125

Uniprot:

P16104

相关产品

Mouse H2afx ELISA Kit

Mouse H2afx CLIA Kit

Mouse H2afx Protein

Mouse H2afx Polyclonal Antibody

Mouse H2afx Monoclonal Antibody

序列长度:

143

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

143:

VTIAQGGVLP | NIQAVLLPKK | TSATVGPKAP | SGGKKATQAS | QEY

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"Tyrosine dephosphorylation of H2AX modulates apoptosis and survival decisions."

(PMID: 19234442)

[2].

"Absence of the H2AX mutations in idiopathic infertile men with spermatogenic impairment."

(PMID: 18536151)

[3].

"Doxorubicin activates ATM-dependent phosphorylation of multiple downstream targets in part through the generation of reactive oxygen species."

(PMID: 15489221)

[4].

"ATM and DNA-PK function redundantly to phosphorylate H2AX after exposure to ionizing radiation."

(PMID: 15059890)

[5].

"MDC1 is a mediator of the mammalian DNA damage checkpoint."

(PMID: 12607005)

[6].

"Accumulation of checkpoint protein 53BP1 at DNA breaks involves its binding to phosphorylated histone H2AX."

(PMID: 12697768)

[7].

"DNA-PK is activated by nucleosomes and phosphorylates H2AX within the nucleosomes in an acetylation-dependent manner."

(PMID: 14627815)

[8].

"A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage."

(PMID: 10959836)

[9].

"Megabase chromatin domains involved in DNA double-strand breaks in vivo."

(PMID: 10477747)

[10].

"Phosphorylation of the Cajal body protein WRAP53β by ATM promotes its involvement in the DNA damage response."

(PMID: 27715493)

[1].

人组蛋白H2A.x(H2AFX)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人组蛋白H2A.x(H2AFX)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人组蛋白H2A.x(H2AFX)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人组蛋白H2A.x(H2AFX)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人组蛋白H2A.x(H2AFX)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人组蛋白H2A.x(H2AFX)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人组蛋白H2A.x(H2AFX)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人组蛋白H2A.x(H2AFX)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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