首页 > 重组蛋白 > Human HAP1 Recombinant Protein

HAP1 (基因名), Huntingtin-associated protein 1 (蛋白名), hap1_human.

产品名称:

Human HAP1/ Huntingtin-associated protein 1 Recombinant Protein
Huntingtin-associated蛋白1

货号:

R13653h

商标:

EIAab^®

监管等级:

RUO

别名:

Neuroan 1, HAP-1, HAP2, HLP1

序列号:

P54257

来源:

E.coli

种属:

Human

标签:

His

序列:

511-671aa

预估分子量:

17.71 kDa (monomer)

纯度:

Greater than 90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

规格 & 价格:

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Human HAP1 Protein

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产品说明书

说明书:

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数据
文献
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

Recombinant human HAP1 protein was determined by 12% SDS-PAGE stained with Coomassie Blue under reducing conditions.

基因位点

Cytogenetic band: 17q21.2 by HGNC 17q21.2 by Entrez Gene 17q21.2 by Ensembl

HAP1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Self-associates. Interacts with HTT/huntingtin; enhanced by an expanded polyglutamine repeat within HTT. Interacts with DCTN1; decreased in presence of HTT with expanded polyglutamine repeat. Interacts with KLC2. Interacts with ITPR1 and APP. Interacts with AR; decreased by an expanded polyglutamine repeat within AR. Interacts with YWHAZ. Interacts with BDNF and SORT1; probably forming a complex involved in proBDNF trafficking, degradation and processing. Interacts with TBP, AHI1, HGS and KALRN. Interacts with KIF5A, KIF5B, KIF5C and GABRB3; indicative for an HAP1:KIF5 complex transporting a GABA(A) receptor as cargo. Interacts with ATXN3; in STBs with ATXN3 poly-Gln region with 27 repeats (normal population) and 79 repeats (spinocerebellar ataxia 3 (SCA3) patients) associating in the same strength. Interacts with NTRK2; HAP1 stabilizes association of NTRK2 with SORT1 preventing NTRK2 degradation. Interacts with CCDC113.

功能:

Originally identified as neuronal protein that specifically associates with HTT/huntingtin and the binding is enhanced by an expanded polyglutamine repeat within HTT possibly affecting HAP1 interaction properties. Both HTT and HAP1 are involved in intracellular trafficking and HAP1 is proposed to link HTT to motor proteins and/or transport cargos. Seems to play a role in vesicular transport within neurons and axons such as from early endosomes to late endocytic compartments and to promote neurite outgrowth. The vesicular transport function via association with microtubule-dependent transporters can be attenuated by association with mutant HTT. Involved in the axonal transport of BDNF and its activity-dependent secretion; the function seems to involve HTT, DCTN1 and a complex with SORT1. Involved in APP trafficking and seems to faciltate APP anterograde transport and membrane insertion thereby possibly reducing processing into amyloid beta. Involved in delivery of gamma-aminobutyric acid (GABA(A)) receptors to synapses; the function is dependent on kinesin motor protein KIF5 and is disrupted by HTT with expanded polyglutamine repeat. Involved in regulation of autophagosome motility by promoting efficient retrograde axonal transport. Seems to be involved in regulation of membrane receptor recycling and degradation, and respective signal transduction, including GABA(A) receptors, tyrosine kinase receptors, EGFR, IP3 receptor and androgen receptor. Among others suggested to be involved in control of feeding behavior (involving hypothalamic GABA(A) receptors), cerebellar and brainstem development (involving AHI1 and NTRK1/TrkA), postnatal neurogenesis (involving hypothalamic NTRK2/TrkB), and ITPR1/InsP3R1-mediated Ca(2+) release (involving HTT and possibly the effect of mutant HTT). Via association with DCTN1/dynactin p150-glued and HTT/huntingtin involved in cytoplasmic retention of REST in neurons. May be involved in ciliogenesis. Involved in regulation of exocytosis. Seems to be involved in formation of cytoplasmic inclusion bodies (STBs). In case of anomalous expression of TBP, can sequester a subset of TBP into STBs; sequestration is enhanced by an expanded polyglutamine repeat within TBP. HAP1-containing STBs have been proposed to play a protective role against neurodegeneration in Huntigton disease (HD) and spinocerebellar ataxia 17 (SCA17).

亚细胞位置:

Cytoplasm Cell projection Axon Cytoplasm Cytoskeleton Lysosome Endoplasmic reticulum Cytoplasmic vesicle Secretory vesicle Synaptic vesicle Mitochondrion Nucleus Cytoplasmic vesicle Autophagosome Localizes to large nonmembrane-bound cytoplasmic bodies found in various types of neurons, called stigmoid bodies (STBs). Localization to neuronal processes and neurite tips is decreased by YWHAZ. In the nucleus localizes to nuclear rods (By similarity).

数据库链接

UniGene:

Hs.158300

SMR:

P54257

KEGG:

hsa:9001

MIM:

600947

Pfam:

PF04849

Uniprot:

P54257

相关产品

序列长度:

671

序列:

仅供参考，详细请咨询（services@eiaab.com）

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671:

GECPHGALPA | ASRTSCRSSC | R

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"Huntingtin-associated protein-1 is a modifier of the age-at-onset of Huntington's disease."

(PMID: 18192679)

[2].

"A human HAP1 homologue. Cloning, expression, and interaction with huntingtin."

(PMID: 9668110)

[3].

"A huntingtin-associated protein enriched in brain with implications for pathology."

(PMID: 7477378)

[4].

"Human huntingtin-associated protein (HAP-1) gene: genomic organisation and an intragenic polymorphism."

(PMID: 10974549)

[5].

"The cellular and subcellular localization of huntingtin-associated protein 1 (HAP1): comparison with huntingtin in rat and human."

(PMID: 9742138)

[6].

"Proteomic analysis of mammalian sperm cells identifies new components of the centrosome."

(PMID: 25074808)

[7].

"The Joubert syndrome-associated missense mutation (V443D) in the Abelson-helper integration site 1 (AHI1) protein alters its localization and protein-protein interactions."

(PMID: 23532844)

[8].

"Huntingtin associated protein 1 regulates trafficking of the amyloid precursor protein and modulates amyloid beta levels in neurons."

(PMID: 22731248)

[9].

"Interaction of ataxin-3 with huntingtin-associated protein 1 through Josephin domain."

(PMID: 21386698)

[10].

"Huntingtin-associated protein-1 interacts with pro-brain-derived neurotrophic factor and mediates its transport and release."

(PMID: 19996106)

[1].

人Huntingtin-associated蛋白1(HAP1)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人Huntingtin-associated蛋白1(HAP1)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人Huntingtin-associated蛋白1(HAP1)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人Huntingtin-associated蛋白1(HAP1)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人Huntingtin-associated蛋白1(HAP1)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人Huntingtin-associated蛋白1(HAP1)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人Huntingtin-associated蛋白1(HAP1)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人Huntingtin-associated蛋白1(HAP1)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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