HDAC2 (基因名), Histone deacetylase 2 (蛋白名), hdac2_human.
产品名称:
Human HDAC2/ Histone deacetylase 2 Recombinant Protein
组蛋白脱乙酰酶2
货号:
R15286h
商标:
EIAab®
监管等级:
别名:
HD2
序列号:
Q92769
来源:
E.coli
种属:
Human
标签:
His
序列:
331-488aa
预估分子量:
17.38 kDa (monomer)
纯度:
Greater than 90% by SDS-PAGE
浓度:
Reconstitution Dependent
形态:
Liquid
内毒素水平:
Please contact protein@eiaab.com The technician for more information.
应用:
存储缓冲液:
50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)
存储:
Store at -20°C. (Avoid repeated freezing and thawing.)
研究领域:
Cancer
R&D 技术数据
Recombinant human HDAC2 protein was determined by 12% SDS-PAGE stained with Coomassie Blue under reducing conditions.
基因位点
HDAC2 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
通用注释
亚单元:
Part of the core histone deacetylase (HDAC) complex composed of HDAC1, HDAC2, RBBP4 and RBBP7. The core complex associates with MTA2, MBD3, MTA1L1, CHD3 and CHD4 to form the nucleosome remodeling and histone deacetylation (NuRD) complex, or with SIN3, SAP18 and SAP30 to form the SIN3 HDAC complex. Component of a RCOR/GFI/KDM1A/HDAC complex. Interacts directly with GFI1 and GFI1B. Interacts with SNW1, HDAC7, PRDM6, SAP30, SETDB1 and SUV39H1. Interacts with the H2AFY (via the non-histone region). Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B, KDM1A, RCOR1 and PHF21A. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. Part of a complex containing the core histones H2A, H2B, H3 and H4, DEK and unphosphorylated DAXX. Part of a complex containing ATR and CHD4. Forms a heterologous complex at least with YY1. Interacts with ATR, CBFA2T3, DNMT1, MINT, HDAC10, HCFC1, NRIP1, KDM4A and PELP1. Component of a mSin3A corepressor complex that contains SIN3A, SAP130, SUDS3, ARID4B, HDAC1 and HDAC2. Interacts with CHFR and SAP30L. Interacts (CK2 phosphorylated form) with SP3. Interacts with TSHZ3 (via its N-terminus). Interacts with APEX1; the interaction is not dependent on the acetylated status of APEX1. Part of a complex composed of TRIM28, HDAC1, HDAC2 and EHMT2. Interacts with FAM64A. Interacts with BCL6 (non-acetylated form). Part of a complex containing at least CDYL, MIER1, MIER2, HDAC1 and HDAC2. Interacts with CRY1, INSM1 and ZNF431. Interacts with NACC2. Interacts with MTA1, with a preference for sumoylated MTA1. Interacts with SIX3 (By similarity). Interacts with BEND3. Component of a histone deacetylase complex containing DNTTIP1, ZNF541, HDAC1 and HDAC2 (PubMed:21573134).
功能:
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. May be involved in the transcriptional repression of circadian target genes, such as PER1, mediated by CRY1 through histone deacetylation. Involved in MTA1-mediated transcriptional corepression of TFF1 and CDKN1A.
亚细胞位置:
Nucleus
Cytoplasm
该产品尚未在任何出版物中被引用。
[1].
"The transcriptional repressor Sp3 is associated with CK2-phosphorylated histone deacetylase 2."
[2].
"DNMT1 binds HDAC2 and a new co-repressor, DMAP1, to form a complex at replication foci."
[3].
"Molecular association between ATR and two components of the nucleosome remodeling and deacetylating complex, HDAC2 and CHD4."
[4].
"Human histone deacetylase 2, HDAC2 (Human RPD3), is localized to 6q21 by radiation hybrid mapping."
[5].
"The subcellular distribution and function of MTA1 in cancer differentiation."
[6].
"RBB, a novel transcription repressor, represses the transcription of HDM2 oncogene."
[7].
"Maintenance of silent chromatin through replication requires SWI/SNF-like chromatin remodeler SMARCAD1."
[8].
"A BEN-domain-containing protein associates with heterochromatin and represses transcription."
[9].
"SUMOylation and SUMO-interacting motif (SIM) of metastasis tumor antigen 1 (MTA1) synergistically regulate its transcriptional repressor function."
[10].
"Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study."
[1].
人组蛋白脱乙酰酶2(HDAC2)重组蛋白是否是无菌的?
蛋白试剂瓶和蛋白保存液是经过高压灭菌的,但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的,可以用0.2微米的滤器对蛋白进行过滤。
[2].
人组蛋白脱乙酰酶2(HDAC2)重组蛋白的保存缓冲液是什么?
纯化后的蛋白保存在PBS(58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4)里,并往里面加入500mM咪唑和10%甘油。
[3].
怎样确定人组蛋白脱乙酰酶2(HDAC2)重组蛋白的浓度?
蛋白浓度的确定没有一个统一的标准,这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。
[4].
人组蛋白脱乙酰酶2(HDAC2)重组蛋白蛋白保存条件是怎样的?
蛋白应保存在 -20℃或 -80℃条件下,为了避免反复冻融,可以将蛋白分装成小份保存。
[5].
人组蛋白脱乙酰酶2(HDAC2)重组蛋白是否可以用于活体实验?
重组蛋白没有用于任何的活体实验,因此蛋白的活性和半衰期是不确定的。
[6].
人组蛋白脱乙酰酶2(HDAC2)重组蛋白的保质期是多久?
在适当的保存条件下,从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃,保证蛋白的保存浓度高于0.1mg/ml,限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题,请联系我们的技术支持。
[7].
你们蛋白和抗体的报价是怎么样的?
我们将根据你需要的蛋白和抗体的大小进行报价。
[8].
人组蛋白脱乙酰酶2(HDAC2)重组蛋白是否能够提供蛋白片段?
我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分,我们将会按您的需求提供蛋白和抗体。
[9].
人组蛋白脱乙酰酶2(HDAC2)重组蛋白的货期或发货时间一般是多长?
具体指标的货期需要确定。最快一周,最长可能一个月。
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