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HNRNPK (基因名), Heterogeneous nuclear ribonucleoprotein K (蛋白名), hnrpk_human.

产品名称:

Human HNRNPK/ Heterogeneous nuclear ribonucleoprotein K Recombinant Protein
异质核核糖蛋白K

货号:

R0351h

商标:

EIAab^®

监管等级:

RUO

别名:

Transformation up-regulated nuclear protein, TUNP, hnRNP K, HNRPK

序列号:

P61978

来源:

E.coli

种属:

Human

标签:

His

序列:

35-197aa

预估分子量:

17.93 kDa (monomer)

纯度:

>90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Epigenetics

规格 & 价格:

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Human HNRNPK Protein

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产品说明书

说明书:

MSDS:

在线询价

数据
文献
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

更多信息，请参阅手册，或联系我们的技术支持: tech@eiaab.com.

基因位点

Cytogenetic band: 9q21.32 by HGNC 9q21.32 by Entrez Gene 9q21.32 by Ensembl

HNRNPK Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Interacts with RBM42 and ZIK1. Interacts with BRDT (By similarity). Identified in the spliceosome C complex. Interacts with ANKRD28. Interacts with HCV core protein. Interacts with ASFV p30 protein. Interacts with DDX1. Interacts with MDM2; this interaction leads to ubiquitination and proteasomal degradation. Interacts with p53/TP53.

功能:

One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction (By similarity). As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest.

亚细胞位置:

Cytoplasm Nucleus Nucleoplasm Cell projection Podosome Recruited to p53/TP53-responsive promoters, in the presence of functional p53/TP53 (PubMed:16360036). In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear (PubMed:18775702).

数据库链接

UniGene:

Hs.522257

SMR:

P61978

STRING:

9606.ENSP00000365439

KEGG:

hsa:3190

MIM:

600712

Pfam:

PF00013

Uniprot:

P61978

相关产品

Mouse Hnrnpk ELISA Kit

Rat Hnrnpk ELISA Kit

Bovine HNRNPK ELISA Kit

Chicken HNRNPK ELISA Kit

Mouse Hnrnpk CLIA Kit

序列长度:

463

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

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463:

LQNSVKQYSG | KFF

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"PITK, a PP1 targeting subunit that modulates the phosphorylation of the transcriptional regulator hnRNP K."

(PMID: 16564677)

[2].

"hnRNP K: an HDM2 target and transcriptional coactivator of p53 in response to DNA damage."

(PMID: 16360036)

[3].

"Hepatitis C virus core protein interacts with heterogeneous nuclear ribonucleoprotein K."

(PMID: 9651361)

[4].

"Identification, molecular cloning, expression and chromosome mapping of a family of transformation upregulated hnRNP-K proteins derived by alternative splicing."

(PMID: 8107114)

[5].

"Characterization and primary structure of the poly(C)-binding heterogeneous nuclear ribonucleoprotein complex K protein."

(PMID: 1729596)

[6].

"GeneMatcher aids in the identification of a new malformation syndrome with intellectual disability, unique facial dysmorphisms, and skeletal and connective tissue abnormalities caused by de novo variants in HNRNPK."

(PMID: 26173930)

[7].

"Cellular RNA binding proteins NS1-BP and hnRNP K regulate influenza A virus RNA splicing."

(PMID: 23825951)

[8].

"DNA damage-induced heterogeneous nuclear ribonucleoprotein K sumoylation regulates p53 transcriptional activation."

(PMID: 22825850)

[9].

"HnRNP K and PDI marked response to chemotherapy to human colorectal cancer cells."

(PMID: 20408130)

[10].

"Identification of heterogeneous nuclear ribonucleoprotein K as a transactivator for human low density lipoprotein receptor gene transcription."

(PMID: 20371611)

[1].

人异质核核糖蛋白K(HNRNPK)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人异质核核糖蛋白K(HNRNPK)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人异质核核糖蛋白K(HNRNPK)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人异质核核糖蛋白K(HNRNPK)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人异质核核糖蛋白K(HNRNPK)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人异质核核糖蛋白K(HNRNPK)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人异质核核糖蛋白K(HNRNPK)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人异质核核糖蛋白K(HNRNPK)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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