首页 > 重组蛋白 > Human HSPB1 Recombinant Protein

HSPB1 (基因名), Heat shock protein beta-1 (蛋白名), hspb1_human.

产品名称:

Human HSPB1/ Heat shock protein beta-1 Recombinant Protein
热休克蛋白27

货号:

R0693h

商标:

EIAab^®

监管等级:

RUO

别名:

28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, Stress-responsive protein 27, HSP 27, SRP27, HspB1, HSP27, HSP28

序列号:

P04792

来源:

E.coli

种属:

Human

标签:

His

序列:

1-205aa

预估分子量:

22.55 kDa (monomer)

纯度:

>90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Epigenetics

规格 & 价格:

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Human HSPB1 Protein

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产品说明书

说明书:

MSDS:

在线询价

数据
文献 ( 1 )
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

The PCR product of human HSPB1 gene was determined by 1% Agarose stained with EB.

Recombinant human HSPB1 protein was determined by 12% SDS-PAGE stained with Coomassie Blue under reducing conditions.

基因位点

Cytogenetic band: 7q11.23 by HGNC 7q11.23 by Entrez Gene 7q11.23 by Ensembl

HSPB1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Homooligomer (PubMed:10383393). Homodimer; becomes monomeric upon activation (PubMed:20178975). Heterooligomer; with HSPB6 (PubMed:23948568). Associates with alpha- and beta-tubulin (PubMed:10777697). Interacts with TGFB1I1 (By similarity). Interacts with CRYAB (PubMed:1560006). Interacts with HSPB8 (PubMed:11342557). Interacts with HSPBAP1 (PubMed:10751411).

功能:

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975). Plays a role in stress resistance and actin organization (PubMed:19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742).

亚细胞位置:

Cytoplasm Nucleus Cytoplasm Cytoskeleton Spindle Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.

数据库链接

UniGene:

Hs.520973

SMR:

P04792

STRING:

9606.ENSP00000248553

KEGG:

hsa:3315

MIM:

602195

Pfam:

PF00011

Uniprot:

P04792

相关产品

Mouse Hspb1 ELISA Kit

Rat Hspb1 ELISA Kit

Bovine HSPB1 ELISA Kit

Chicken HSPB1 ELISA Kit

Pig HSPB1 ELISA Kit

序列长度:

205

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

205:

ETAAK

3D 模型:

3D 结构:

免疫原序列:

验证序列:

[1].

"Fractional excretion as a new marker of tubular damage in children with chronic kidney disease"

K Musiał, D Zwolińska.

From: Clinica Chimica Acta

[1].

"PKA-induced F-actin rearrangement requires phosphorylation of Hsp27 by the MAPKAP kinase MK5."

(PMID: 19166925)

[2].

"Mutations in the HSP27 (HSPB1) gene cause dominant, recessive, and sporadic distal HMN/CMT type 2."

(PMID: 18832141)

[3].

"HSP22, a new member of the small heat shock protein superfamily, interacts with mimic of phosphorylated HSP27 ((3D)HSP27)."

(PMID: 11342557)

[4].

"Small heat shock proteins are molecular chaperones."

(PMID: 8093612)

[5].

"Identification of MAPKAP kinase 2 as a major enzyme responsible for the phosphorylation of the small mammalian heat shock proteins."

(PMID: 1332886)

[6].

"Human HSP27 is phosphorylated at serines 78 and 82 by heat shock and mitogen-activated kinases that recognize the same amino acid motif as S6 kinase II."

(PMID: 1730670)

[7].

"The 29-kDa proteins phosphorylated in thrombin-activated human platelets are forms of the estrogen receptor-related 27-kDa heat shock protein."

(PMID: 1763035)

[8].

"cDNA sequence of a human heat shock protein HSP27."

(PMID: 2243808)

[9].

"Axonal Neuropathies due to Mutations in Small Heat Shock Proteins: Clinical, Genetic, and Functional Insights into Novel Mutations."

(PMID: 28144995)

[10].

"Characterization of Mutants of Human Small Heat Shock Protein HspB1 Carrying Replacements in the N-Terminal Domain and Associated with Hereditary Motor Neuron Diseases."

(PMID: 25965061)

[1].

人热休克蛋白27(HSPB1)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人热休克蛋白27(HSPB1)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人热休克蛋白27(HSPB1)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人热休克蛋白27(HSPB1)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人热休克蛋白27(HSPB1)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人热休克蛋白27(HSPB1)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人热休克蛋白27(HSPB1)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人热休克蛋白27(HSPB1)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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