首页 > 重组蛋白 > Human HUWE1 Recombinant Protein

HUWE1 (基因名), E3 ubiquitin-protein ligase HUWE1 (蛋白名), huwe1_human.

产品名称:

Human HUWE1/ E3 ubiquitin-protein ligase HUWE1 Recombinant Protein
E3泛素蛋白连接酶HUWE1

货号:

R2369h

商标:

EIAab^®

监管等级:

RUO

别名:

ARF-binding protein 1, HECT, UBA and WWE domain-containing protein 1, HECT-type E3 ubiquitin transferase HUWE1, Homologous to E6AP carboxyl terminus homologous protein 9, Large structure of UREB1, Mcl-1 ubiquitin ligase E3, Upstream regulatory element-binding protein 1, ARF-BP1, HectH9, LASU1, Mule, URE-B1, HSPC272, KIAA0312, KIAA1578, UREB1

序列号:

Q7Z6Z7

来源:

E.coli

种属:

Human

标签:

His

序列:

Recombinant protein

纯度:

>90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

规格 & 价格:

关注

Human HUWE1 Protein

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产品说明书

说明书:

MSDS:

在线询价

数据
文献
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

更多信息，请参阅手册，或联系我们的技术支持: tech@eiaab.com.

基因位点

Cytogenetic band: Xp11.22 by HGNC Xp11.22 by Entrez Gene Xp11.22 by Ensembl

HUWE1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Interacts with isoform p14ARF of CDKN2A which strongly inhibits HUWE1 ubiquitin ligase activity. Interacts with MYCN, POLB and CDC6. Interacts with isoform 2 of PA2G4.

功能:

E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. Regulates apoptosis by catalyzing the polyubiquitination and degradation of MCL1. Mediates monoubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair. Also ubiquitinates the p53/TP53 tumor suppressor and core histones including H1, H2A, H2B, H3 and H4. Binds to an upstream initiator-like sequence in the preprodynorphin gene. Regulates neural differentiation and proliferation by catalyzing the polyubiquitination and degradation of MYCN. May regulate abundance of CDC6 after DNA damage by polyubiquitinating and targeting CDC6 to degradation. Mediates polyubiquitination of isoform 2 of PA2G4.

亚细胞位置:

Cytoplasm Nucleus Mainly expressed in the cytoplasm of most tissues, except in the nucleus of spermatogonia, primary spermatocytes and neuronal cells (By similarity). Predominantly cytosolic or perinuclear in some colorectal carcinoma cells.

数据库链接

UniGene:

Hs.136905

SMR:

Q7Z6Z7

STRING:

9606.ENSP00000262854

KEGG:

hsa:10075

MIM:

300697

Pfam:

PF14377

Uniprot:

Q7Z6Z7

相关产品

Mouse Huwe1 ELISA Kit

序列长度:

4374

序列:

仅供参考，详细请咨询（services@eiaab.com）

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ESFEKLRHML | LLAIQECSEG | FGLA

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"Mule/ARF-BP1, a BH3-only E3 ubiquitin ligase, catalyzes the polyubiquitination of Mcl-1 and regulates apoptosis."

(PMID: 15989957)

[2].

"ARF-BP1/Mule is a critical mediator of the ARF tumor suppressor."

(PMID: 15989956)

[3].

"Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro."

(PMID: 9205841)

[4].

"Ubiquitin ligase ARF-BP1/Mule modulates base excision repair."

(PMID: 19713937)

[5].

"The HECT-domain ubiquitin ligase Huwe1 controls neural differentiation and proliferation by destabilizing the N-Myc oncoprotein."

(PMID: 18488021)

[1].

人E3泛素蛋白连接酶HUWE1(HUWE1)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人E3泛素蛋白连接酶HUWE1(HUWE1)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人E3泛素蛋白连接酶HUWE1(HUWE1)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人E3泛素蛋白连接酶HUWE1(HUWE1)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人E3泛素蛋白连接酶HUWE1(HUWE1)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人E3泛素蛋白连接酶HUWE1(HUWE1)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人E3泛素蛋白连接酶HUWE1(HUWE1)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人E3泛素蛋白连接酶HUWE1(HUWE1)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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Human HUWE1/ E3 ubiquitin-protein ligase HUWE1 Recombinant ProteinE3泛素蛋白连接酶HUWE1

R2369h

Human HUWE1/ E3 ubiquitin-protein ligase HUWE1 Recombinant Protein
E3泛素蛋白连接酶HUWE1