首页 > 重组蛋白 > Human KDM1A Recombinant Protein

KDM1A (基因名), Lysine-specific histone demethylase 1A (蛋白名), kdm1a_human.

产品名称:

Human KDM1A/ Lysine-specific histone demethylase 1A Recombinant Protein
赖氨酸特异组蛋白去甲基化酶1A

货号:

R12881h

商标:

EIAab^®

监管等级:

RUO

别名:

BRAF35-HDAC complex protein BHC110, Flavin-containing amine oxidase domain-containing protein 2, AOF2, KDM1, KIAA0601, LSD1

序列号:

O60341

来源:

E.coli

种属:

Human

标签:

His

纯度:

>90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

规格 & 价格:

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Human KDM1A Protein

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产品说明书

说明书:

MSDS:

在线询价

数据
文献
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

更多信息，请参阅手册，或联系我们的技术支持: tech@eiaab.com.

基因位点

Cytogenetic band: 1p36.12 by HGNC 1p36.12 by Entrez Gene 1p36.12 by Ensembl

KDM1A Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Component of a RCOR/GFI/KDM1A/HDAC complex. Interacts directly with GFI1 and GFI1B. Interacts with INSM1 (By similarity). Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B, KDM1A, RCOR1 and PHF21A. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. In the complex, RCOR1/CoREST strongly enhances the demethylase activity and protects it from the proteasome while PHF21A/BHC80 inhibits the demethylase activity. Interacts with the androgen receptor (AR). Interacts with ASXL1. Interacts with SNAI1 (via SNAG domain).

功能:

Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Effector of SNAI1-mediated transcription repression of E-cadherin/CDH1, CDN7 and KRT8. Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7.

亚细胞位置:

Nucleus

数据库链接

UniGene:

Hs.591518

SMR:

O60341

STRING:

9606.ENSP00000383042

KEGG:

hsa:23028

MIM:

609132

Pfam:

PF01593

Uniprot:

O60341

相关产品

Mouse Kdm1a ELISA Kit

Mouse Kdm1a CLIA Kit

Mouse Kdm1a Protein

Mouse Kdm1a Polyclonal Antibody

Mouse Kdm1a Monoclonal Antibody

序列长度:

852

序列:

仅供参考，详细请咨询（services@eiaab.com）

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3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"A candidate X-linked mental retardation gene is a component of a new family of histone deacetylase-containing complexes."

(PMID: 12493763)

[2].

"p53 is regulated by the lysine demethylase LSD1."

(PMID: 17805299)

[3].

"Solution structure of the SWIRM domain of human histone demethylase LSD1."

(PMID: 16531230)

[4].

"Structural basis for CoREST-dependent demethylation of nucleosomes by the human LSD1 histone demethylase."

(PMID: 16885027)

[5].

"Crystal structure of human histone lysine-specific demethylase 1 (LSD1)."

(PMID: 16956976)

[6].

"Histone demethylation catalysed by LSD1 is a flavin-dependent oxidative process."

(PMID: 15811342)

[7].

"An essential role for CoREST in nucleosomal histone 3 lysine 4 demethylation."

(PMID: 16079794)

[8].

"LSD1 demethylates repressive histone marks to promote androgen-receptor-dependent transcription."

(PMID: 16079795)

[9].

"Regulation of LSD1 histone demethylase activity by its associated factors."

(PMID: 16140033)

[10].

"Stable histone deacetylase complexes distinguished by the presence of SANT domain proteins CoREST/kiaa0071 and Mta-L1."

(PMID: 11102443)

[1].

人赖氨酸特异组蛋白去甲基化酶1A(KDM1A)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人赖氨酸特异组蛋白去甲基化酶1A(KDM1A)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人赖氨酸特异组蛋白去甲基化酶1A(KDM1A)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人赖氨酸特异组蛋白去甲基化酶1A(KDM1A)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人赖氨酸特异组蛋白去甲基化酶1A(KDM1A)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人赖氨酸特异组蛋白去甲基化酶1A(KDM1A)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人赖氨酸特异组蛋白去甲基化酶1A(KDM1A)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人赖氨酸特异组蛋白去甲基化酶1A(KDM1A)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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