首页 > 重组蛋白 > Human PKM Recombinant Protein

PKM (基因名), Pyruvate kinase PKM (蛋白名), kpym_human.

产品名称:

Human PKM/ Pyruvate kinase PKM Recombinant Protein
丙酮酸激酶同工酶M1/M2

货号:

R0588h

商标:

EIAab^®

监管等级:

RUO

别名:

Cytosolic thyroid hormone-binding protein, Opa-interacting protein 3, Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, Thyroid hormone-binding protein 1, Tumor M2-PK, p58, CTHBP, OIP-3, THBP1, OIP3, PK2, PK3, PKM2

序列号:

P14618

来源:

E.coli

种属:

Human

标签:

His

序列:

81-280aa

预估分子量:

22 kDa (monomer)

纯度:

Greater than 90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Epigenetics

规格 & 价格:

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Human PKM Protein

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产品说明书

说明书:

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数据
文献
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

Recombinant human PKM protein was determined by 12% SDS-PAGE stained with Coomassie Blue under reducing conditions.

基因位点

Cytogenetic band: 15q23 by HGNC 15q23 by Entrez Gene 15q23 by Ensembl

PKM Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Monomer and homotetramer. Exists as a monomer in the absence of D-fructose 1,6-bisphosphate (FBP), and reversibly associates to form a homotetramer in the presence of FBP. The monomeric form binds T3. Tetramer formation induces pyruvate kinase activity. The tetrameric form has high affinity for the substrate and is associated within the glycolytic enzyme complex. Exists in a nearly inactive dimeric form in tumor cells and the dimeric form has less affinity for the substrate. Binding to certain oncoproteins such as HPV-16 E7 oncoprotein can trigger dimerization. FBP stimulates the formation of tetramers from dimers. Interacts with HERC1, POU5F1 and PML. Interacts (isoform M2) with EGLN3; the interaction hydroxylates PKM under hypoxia and enhances binding to HIF1A. Interacts (isoform M2) with HIF1A; the interaction is enhanced by binding of EGLN3, promoting enhanced transcription activity under hypoxia. Interacts (isoform M2, but not isoform M1) with TRIM35; this interaction prevents FGFR1-dependent tyrosine phosphorylation (PubMed:25263439).

功能:

Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.

亚细胞位置:

Cytoplasm Nucleus Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity.

数据库链接

UniGene:

Hs.534770

SMR:

P14618

STRING:

9606.ENSP00000320171

KEGG:

hsa:5315

MIM:

179050

Pfam:

PF00224

Uniprot:

P14618

相关产品

序列长度:

531

序列:

仅供参考，详细请咨询（services@eiaab.com）

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531:

GFFKKGDVVI | VLTGWRPGSG | FTNTMRVVPV | P

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"Pyruvate kinase M2 is a phosphotyrosine-binding protein."

(PMID: 18337815)

[2].

"Modulation of M2-type pyruvate kinase activity by the cytoplasmic PML tumor suppressor protein."

(PMID: 18298799)

[3].

"Pyruvate kinase isozyme type M2 (PKM2) interacts and cooperates with Oct-4 in regulating transcription."

(PMID: 18191611)

[4].

"Nuclear translocation of the tumor marker pyruvate kinase M2 induces programmed cell death."

(PMID: 17308100)

[5].

"Structural basis for tumor pyruvate kinase M2 allosteric regulation and catalysis."

(PMID: 15996096)

[6].

"High glucose upregulates pantothenate kinase 4 (PanK4) and thus affects M2-type pyruvate kinase (Pkm2)."

(PMID: 16132722)

[7].

"Interaction between HERC1 and M2-type pyruvate kinase."

(PMID: 12650930)

[8].

"Isolation and characterization of the human pyruvate kinase M gene."

(PMID: 2040271)

[9].

"JMJD8 Regulates Angiogenic Sprouting and Cellular Metabolism by Interacting With Pyruvate Kinase M2 in Endothelial Cells."

(PMID: 27199445)

[10].

"TRIM35 Interacts with pyruvate kinase isoform M2 to suppress the Warburg effect and tumorigenicity in hepatocellular carcinoma."

(PMID: 25263439)

[1].

人丙酮酸激酶同工酶M1/M2(PKM)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人丙酮酸激酶同工酶M1/M2(PKM)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人丙酮酸激酶同工酶M1/M2(PKM)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人丙酮酸激酶同工酶M1/M2(PKM)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人丙酮酸激酶同工酶M1/M2(PKM)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人丙酮酸激酶同工酶M1/M2(PKM)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人丙酮酸激酶同工酶M1/M2(PKM)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人丙酮酸激酶同工酶M1/M2(PKM)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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