首页 > 重组蛋白 > Human LATS1 Recombinant Protein

LATS1 (基因名), Serine/threonine-protein kinase LATS1 (蛋白名), lats1_human.

产品名称:

Human LATS1/ Serine/threonine-protein kinase LATS1 Recombinant Protein
丝氨酸/苏氨酸蛋白激酶LATS1

货号:

R14087h

商标:

EIAab^®

监管等级:

RUO

别名:

Large tumor suppressor homolog 1, WARTS protein kinase, h-warts, WARTS

序列号:

O95835

来源:

E.coli

种属:

Human

标签:

His

序列:

705-1010aa

预估分子量:

33.66 kDa (monomer)

纯度:

>90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

规格 & 价格:

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Human LATS1 Protein

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产品说明书

说明书:

MSDS:

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数据
文献
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

Recombinant human LATS1 protein was determined by 12% SDS-PAGE stained with Coomassie Blue under reducing conditions.

基因位点

Cytogenetic band: 6q25.1 by HGNC 6q25.1 by Entrez Gene 6q25.1 by Ensembl

LATS1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Complexes with CDK1 in early mitosis. LATS1-associated CDK1 has no mitotic cyclin partner and no apparent kinase activity. Binds phosphorylated ZYX, locating this protein to the mitotic spindle and suggesting a role for actin regulatory proteins during mitosis. Binds to and colocalizes with LIMK1 at the actomyosin contractile ring during cytokinesis. Interacts (via PPxY motif 2) with YAP1 (via WW domains). Interacts with MOB1A and MOB1B. Interacts with LIMD1, WTIP and AJUBA. Interacts with ESR1, VPRBP and DCAF13; probably recruits VPRBP and DCAF13 to ESR1 to promote ESR1 ubiquitination and ubiquitin-mediated proteasomal degradation (PubMed:28068668).

功能:

Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function. Plays a role in mammary gland epithelial cells differentiation, both through the Hippo signaling pathway and the intracellular estrogen receptor signaling pathway by promoting the degradation of ESR1 (PubMed:28068668).

亚细胞位置:

Cytoplasm Cytoskeleton Microtubule organizing center Centrosome Localizes to the centrosomes throughout interphase but migrates to the mitotic apparatus, including spindle pole bodies, mitotic spindle, and midbody, during mitosis.

数据库链接

UniGene:

Hs.549084

SMR:

O95835

STRING:

9606.ENSP00000253339

KEGG:

hsa:9113

MIM:

603473

Pfam:

PF00069

Uniprot:

O95835

相关产品

Mouse Lats1 ELISA Kit

Mouse Lats1 CLIA Kit

Mouse Lats1 Protein

Mouse Lats1 Polyclonal Antibody

Mouse Lats1 Monoclonal Antibody

序列长度:

1130

序列:

仅供参考，详细请咨询（services@eiaab.com）

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YINSQGSEQQ | SDEDDQNTGS | EIKNRDLVYV |

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"Molecular characterization of human homologs of yeast MOB1."

(PMID: 19739119)

[2].

"Tumor suppressor WARTS ensures genomic integrity by regulating both mitotic progression and G1 tetraploidy checkpoint function."

(PMID: 15122335)

[3].

"LATS1 tumour suppressor affects cytokinesis by inhibiting LIMK1."

(PMID: 15220930)

[4].

"Human homologue of the Drosophila melanogaster lats tumour suppressor modulates CDC2 activity."

(PMID: 9988268)

[5].

"The Hippo kinases LATS1 and 2 control human breast cell fate via crosstalk with ERα."

(PMID: 28068668)

[1].

人丝氨酸/苏氨酸蛋白激酶LATS1(LATS1)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人丝氨酸/苏氨酸蛋白激酶LATS1(LATS1)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人丝氨酸/苏氨酸蛋白激酶LATS1(LATS1)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人丝氨酸/苏氨酸蛋白激酶LATS1(LATS1)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人丝氨酸/苏氨酸蛋白激酶LATS1(LATS1)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人丝氨酸/苏氨酸蛋白激酶LATS1(LATS1)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人丝氨酸/苏氨酸蛋白激酶LATS1(LATS1)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人丝氨酸/苏氨酸蛋白激酶LATS1(LATS1)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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