首页 > 重组蛋白 > Human LCAT Recombinant Protein

LCAT (基因名), Phosphatidylcholine-sterol acyltransferase (蛋白名), lcat_human.

产品名称:

Human LCAT/ Phosphatidylcholine-sterol acyltransferase Recombinant Protein
Phosphatidylcholine-sterol酰基转移酶

货号:

R2114h

商标:

EIAab^®

监管等级:

RUO

别名:

Lecithin-cholesterol acyltransferase, Phospholipid-cholesterol acyltransferase

序列号:

P04180

来源:

E.coli

种属:

Human

标签:

His

纯度:

>90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Cardiovascular

规格 & 价格:

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Human LCAT Protein

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产品说明书

说明书:

MSDS:

在线询价

数据
文献 ( 1 )
出版物
序列 / 3D
常见问题解答
反馈墙 (0)

R&D 技术数据

更多信息，请参阅手册，或联系我们的技术支持: tech@eiaab.com.

基因位点

Cytogenetic band: 16q22.1 by HGNC 16q22.1 by Entrez Gene 16q22.1 by Ensembl

LCAT Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

N/A

功能:

Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs) (PubMed:10329423, PubMed:19065001, PubMed:26195816). The cholesterol ester is then transported back to the liver. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines (PubMed:8820107). Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms (PubMed:10722751).

亚细胞位置:

Secreted Secreted into blood plasma (PubMed:3458198, PubMed:8820107, PubMed:10222237). Produced in astrocytes and secreted into cerebral spinal fluid (CSF).

数据库链接

UniGene:

Hs.387239

SMR:

P04180

STRING:

9606.ENSP00000264005

KEGG:

hsa:3931

MIM:

136120

Pfam:

PF02450

Uniprot:

P04180

相关产品

Mouse Lcat ELISA Kit

Rat Lcat ELISA Kit

Chicken LCAT ELISA Kit

Pig LCAT ELISA Kit

Mouse Lcat CLIA Kit

序列长度:

440

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

250:

300:

350:

400:

440:

HLNMVFSNLT | LEHINAILLG | AYRQGPPASP | TASPEPPPPE |

3D 模型:

3D 结构:

免疫原序列:

验证序列:

[1].

"Anticholesterol Activity of Anacardium occidentale Linn. Does it Involve in Reverse Cholesterol Transport?"

MOHD KAMAL NIK HASAN, IHSAN SAFWAN KAMARAZAMAN

From: Sains Malaysiana

[1].

"Genetic analysis of 103 candidate genes for coronary artery disease and associated phenotypes in a founder population reveals a new association between endothelin-1 and high-density lipoprotein cholesterol."

(PMID: 17357073)

[2].

"Familial lecithin-cholesterol acyltransferase deficiency: biochemical characteristics and molecular analysis of a new LCAT mutation in a Polish family."

(PMID: 16051254)

[3].

"The molecular basis of lecithin:cholesterol acyltransferase deficiency syndromes: a comprehensive study of molecular and biochemical findings in 13 unrelated Italian families."

(PMID: 15994445)

[4].

"Combined monogenic hypercholesterolemia and hypoalphalipoproteinemia caused by mutations in LDL-R and LCAT genes."

(PMID: 16115486)

[5].

"Genetic and phenotypic heterogeneity in familial lecithin: cholesterol acyltransferase (LCAT) deficiency. Six newly identified defective alleles further contribute to the structural heterogeneity in this disease."

(PMID: 8432868)

[6].

"Two different allelic mutations in the lecithin-cholesterol acyltransferase gene associated with the fish eye syndrome. Lecithin-cholesterol acyltransferase (Thr123----Ile) and lecithin-cholesterol acyltransferase (Thr347----Met)."

(PMID: 1737840)

[7].

"Lecithin-cholesterol acyltransferase (LCAT) deficiency with a missense mutation in exon 6 of the LCAT gene."

(PMID: 1859405)

[8].

"Lecithin cholesterol acyl transferase deficiency: molecular analysis of a mutated allele."

(PMID: 2370048)

[9].

"The high-resolution crystal structure of human LCAT."

(PMID: 26195816)

[10].

"High prevalence of mutations in LCAT in patients with low HDL cholesterol levels in The Netherlands: identification and characterization of eight novel mutations."

(PMID: 21901787)

[1].

人Phosphatidylcholine-sterol酰基转移酶(LCAT)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人Phosphatidylcholine-sterol酰基转移酶(LCAT)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人Phosphatidylcholine-sterol酰基转移酶(LCAT)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人Phosphatidylcholine-sterol酰基转移酶(LCAT)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人Phosphatidylcholine-sterol酰基转移酶(LCAT)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人Phosphatidylcholine-sterol酰基转移酶(LCAT)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人Phosphatidylcholine-sterol酰基转移酶(LCAT)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人Phosphatidylcholine-sterol酰基转移酶(LCAT)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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