首页 > 重组蛋白 > Mouse Lrp6 Recombinant Protein

Lrp6 (基因名), Low-density lipoprotein receptor-related protein 6 (蛋白名), lrp6_mouse.

产品名称:

Mouse Lrp6/ Low-density lipoprotein receptor-related protein 6 Recombinant Protein
低密度脂蛋白受体相关蛋白6

货号:

R1010m

商标:

EIAab^®

监管等级:

RUO

别名:

LRP-6

序列号:

O88572

来源:

E.coli

种属:

Mouse

标签:

His

序列:

1394-1613aa

预估分子量:

24.2 kDa (monomer)

纯度:

Greater than 98% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Cancer

规格 & 价格:

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Mouse Lrp6 Protein

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产品说明书

说明书:

MSDS:

在线询价

数据
文献
序列 / 3D
常见问题解答
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R&D 技术数据

更多信息，请参阅手册，或联系我们的技术支持: tech@eiaab.com.

通用注释

亚单元:

Homodimer; disulfide-linked (By similarity). Forms phosphorylated oligomer aggregates on Wnt-signaling (By similarity). Component of the Wnt-Fzd-LRP5-LRP6 complex. Interacts (via the extracellular domain) with WNT1; the interaction is enhanced by prior formation of the Wnt/Fzd complex. Interacts (via the beta-propeller regions 3 and 4) with WNT3A. Interacts (via the beta-propeller regions 1 and 2) with WNT9B. Interacts with FZD5; the interaction forms a coreceptor complex for Wnt signaling and is inhibited by DKK1 and DRAXIN. Interacts (via beta propeller region) with DKK1; the interaction inhibits FZD5/LRP6 complex formation. Interacts with DKK2. Interacts (via the phosphorylated PPPSP motifs) with AXIN1; the interaction recruits the AXIN1/GSK3B complex to cell surface LRP6 signalsomes. Interacts (via the extracellular domain) with RSPO1; the interaction activates Wnt/beta-catenin signaling. Interacts (via the extracellular domain) with RSPO3 (via the cysteine rich domain); the interaction activates Wnt/beta-catenin signaling. Interacts (via the beta-propeller regions 1 and 2) with SOST; the interaction competes with DKK1 for binding for inhibiting beta-catenin signaling. Interacts (via the cytoplasmic domain) with CSNKIE; the interaction phosphorylates LRP6, binds AXIN1 and inhibits AXIN1/GSK3B-mediated phosphorylation of beta-catenin (By similarity). Interacts with DRAXIN; the interaction inhibits Wnt signaling. Interacts with GRB10; the interaction prevents AXIN1 binding, thus negatively regulating the Wnt signaling pathway. Interacts with MESD; the interaction prevents the formation of LRP6 aggregates and targets LRP6 to the plasma membrane. Interacts with MACF1. Interacts with DAB2; the interaction involves LRP6 phosphorylation by CK2 and sequesters LRP6 towards clathrin-mediated endocytosis. Interacts with TMEM198 (By similarity). Interacts with CAPRIN2; the interaction promotes LRP6 phosphorylation at Ser-1490 (By similarity). Found in a complex with CAPRIN2, CCNY and CDK14 during G2/M stage; CAPRIN2 functions as a scaffold for the complex by binding to CCNY via its N terminus and to CDK14 via its C terminus. Interacts with LYPD6.

功能:

Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalsomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3-mediated phosphorylation and destruction of beta-catenin. Required for posterior patterning of the epiblast during gastrulation.

亚细胞位置:

Cell membrane Single-pass type I membrane protein Endoplasmic reticulum Membrane raft On Wnt signaling, undergoes a cycle of caveolin- or clathrin-mediated endocytosis and plasma membrane location. Released from the endoplasmic reticulum on palmitoylation. Mono-ubiquitination retains it in the endoplasmic reticulum in the absence of palmitoylation. On Wnt signaling, phosphorylated, aggregates and colocalizes with AXIN1 and GSK3B at the plasma membrane in LRP6-signalsomes (By similarity). Chaperoned to the plasma membrane by MESD.

数据库链接

UniGene:

Mm.321990

SMR:

O88572

STRING:

10090.ENSMUSP00000032322

KEGG:

mmu:16974

Pfam:

PF00057

Uniprot:

O88572

相关产品

Human LRP6 ELISA Kit

Human LRP6 CLIA Kit

Human LRP6 Protein

Human LRP6 Polyclonal Antibody

Human LRP6 Monoclonal Antibody

序列长度:

1613

序列:

仅供参考，详细请咨询（services@eiaab.com）

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LYPPPPSPCT | DSS

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

小鼠低密度脂蛋白受体相关蛋白6(Lrp6)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

小鼠低密度脂蛋白受体相关蛋白6(Lrp6)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定小鼠低密度脂蛋白受体相关蛋白6(Lrp6)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

小鼠低密度脂蛋白受体相关蛋白6(Lrp6)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

小鼠低密度脂蛋白受体相关蛋白6(Lrp6)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

小鼠低密度脂蛋白受体相关蛋白6(Lrp6)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

小鼠低密度脂蛋白受体相关蛋白6(Lrp6)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

小鼠低密度脂蛋白受体相关蛋白6(Lrp6)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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