首页 > 重组蛋白 > Human NME1 Recombinant Protein

NME1 (基因名), Nucleoside diphosphate kinase A (蛋白名), ndka_human.

产品名称:

Human NME1/ Nucleoside diphosphate kinase A Recombinant Protein
二磷酸核苷激酶A

货号:

R14300h

商标:

EIAab^®

监管等级:

RUO

别名:

Granzyme A-activated DNase, Metastasis inhibition factor nm23, NM23-H1, Tumor metastatic process-associated protein, GAAD, NDK A, NDPKA, NM23

序列号:

P15531

来源:

E.coli

种属:

Human

标签:

His

序列:

1-152aa

预估分子量:

16.72 kDa (monomer)

纯度:

Greater than 95% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Cancer

规格 & 价格:

关注

Human NME1 Protein

邮箱 *

消息 *

Please 登录.

产品说明书

说明书:

MSDS:

在线询价

数据
文献
出版物
序列 / 3D
常见问题解答
反馈墙 (0)

R&D 技术数据

The PCR product of human NME1 gene was determined by 1% Agarose stained with EB.

Recombinant human NME1 protein was determined by 12% SDS-PAGE stained with Coomassie Blue under reducing conditions.

基因位点

Cytogenetic band: 17q21.33 by HGNC 17q21.33 by Entrez Gene 17q21.33 by Ensembl

NME1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Hexamer of two different chains: A and B (A6, A5B, A4B2, A3B3, A2B4, AB5, B6). Interacts with PRUNE. Component of the SET complex, composed of at least ANP32A, APEX1, HMGB2, NME1, SET and TREX1. Within this complex, interacts directly with SET. Also interacts with TREX1, but only following translocation to the nucleus.

功能:

Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination. During GZMA-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair.

亚细胞位置:

Cytoplasm Nucleus Cell-cycle dependent nuclear localization which can be induced by interaction with Epstein-barr viral proteins or by degradation of the SET complex by GzmA.

数据库链接

UniGene:

Hs.463456

SMR:

P15531

STRING:

9606.ENSP00000013034

KEGG:

hsa:4830

MIM:

156490

Pfam:

PF00334

Uniprot:

P15531

相关产品

序列长度:

152

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

152:

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"Tumor suppressor NM23-H1 is a granzyme A-activated DNase during CTL-mediated apoptosis, and the nucleosome assembly protein SET is its inhibitor."

(PMID: 12628186)

[2].

"Site-directed mutagenesis of nm23-H1. Mutation of proline 96 or serine 120 abrogates its motility inhibitory activity upon transfection into human breast carcinoma cells."

(PMID: 8810265)

[3].

"Isolation and characterization of the human genomic locus coding for the putative metastasis control gene nm23-H1."

(PMID: 8270257)

[4].

"Centrosome-related genes, genetic variation, and risk of breast cancer."

(PMID: 20508983)

[5].

"Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression."

(PMID: 20877624)

[6].

"NME1 at the human maternal-fetal interface downregulates titin expression and invasiveness of trophoblast cells via MAPK pathway in early pregnancy."

(PMID: 20145075)

[7].

"Significant association of genetic polymorphism of human nonmetastatic clone 23 type 1 gene with an increased risk of endometrial cancer."

(PMID: 20599259)

[8].

"The Nm23-H1 metastasis suppressor as a translational target."

(PMID: 20304626)

[9].

"Nme protein family evolutionary history, a vertebrate perspective."

(PMID: 19852809)

[10].

"Screening and association testing of common coding variation in steroid hormone receptor co-activator and co-repressor genes in relation to breast cancer risk: the Multiethnic Cohort."

(PMID: 19183483)

[1].

人二磷酸核苷激酶A(NME1)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人二磷酸核苷激酶A(NME1)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人二磷酸核苷激酶A(NME1)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人二磷酸核苷激酶A(NME1)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人二磷酸核苷激酶A(NME1)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人二磷酸核苷激酶A(NME1)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人二磷酸核苷激酶A(NME1)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人二磷酸核苷激酶A(NME1)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

反馈墙

评论数 : 0

所有用户