首页 > 重组蛋白 > Human OTULIN Recombinant Protein

OTULIN (基因名), Ubiquitin thioesterase otulin (蛋白名), otul_human.

产品名称:

Human OTULIN/ Ubiquitin thioesterase otulin Recombinant Protein
泛素thioesterase otulin

货号:

R15311h

商标:

EIAab^®

监管等级:

RUO

别名:

Deubiquitinating enzyme otulin, OTU domain-containing deubiquitinase with linear linkage specificity, Ubiquitin thioesterase Gumby, FAM105B

序列号:

Q96BN8

来源:

E.coli

种属:

Human

标签:

His

序列:

1-250aa

预估分子量:

27.5 kDa (monomer)

纯度:

Greater than 90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

规格 & 价格:

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Human OTULIN Protein

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产品说明书

说明书:

MSDS:

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数据
文献
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

Recombinant human OTULIN protein was determined by 12% SDS-PAGE stained with Coomassie Blue under reducing conditions.

基因位点

Cytogenetic band: 5p15.2 by HGNC 5p15.2 by Entrez Gene 5p15.2 by Ensembl

OTULIN Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Interacts (via the PUB domain) with RNF31 (via the PIM motif); the interaction is direct (PubMed:27523608, PubMed:23708998, PubMed:23746843, PubMed:24726323, PubMed:24726327). Interacts with DVL2.

功能:

Deubiquitinase that specifically removes linear ('Met-1'-linked) polyubiquitin chains to substrates and acts as a regulator of angiogenesis and innate immune response (PubMed:23708998, PubMed:23746843, PubMed:23806334, PubMed:23827681, PubMed:27523608, PubMed:27559085, PubMed:24726323, PubMed:24726327). Associates with the LUBAC complex via direct interaction with RNF31 and counteracts its action by cleaving linear polyubiquitin chains to substrates (PubMed:23708998, PubMed:23746843, PubMed:23806334, PubMed:23827681, PubMed:24726323, PubMed:24726327). Required during angiogenesis, craniofacial and neuronal development by regulating the canonical Wnt signaling together with the LUBAC complex (PubMed:23708998). Acts as a negative regulator of NF-kappa-B by counteracting activity of the LUBAC complex (PubMed:23746843, PubMed:23806334). Required for homeostasis of the LUBAC complex by restricting autoubiquination of the LUBAC complex subunit RNF31 (PubMed:24726323). Some results have suggested that OTULIN function is restricted to homeostasis of the LUBAC complex, because it is not stably associated with TNF or NOD2 receptor signaling complexes (RSCs) (PubMed:26670046). However, further report have shown that it plays active roles in receptor signaling (PubMed:26997266, PubMed:27523608). Acts as a key negative regulator of inflammation by restricting spontaneous inflammation and maintaining immune homeostasis (PubMed:27523608). In myeloid cell, required to prevent unwarranted secretion of cytokines leading to inflammation and autoimmunity by restricting linear polyubiquitin formation (PubMed:27523608). Plays a key role in innate immune response by restricting linear polyubiquitin formation on RIPK2 in response to NOD2 stimulation, probably to limit NOD2-dependent proinflammatory signaling (PubMed:23806334).

亚细胞位置:

Cytoplasm

数据库链接

UniGene:

Hs.406335

SMR:

Q96BN8

STRING:

9606.ENSP00000284274

KEGG:

hsa:90268

MIM:

615712

Pfam:

PF16218

Uniprot:

Q96BN8

相关产品

Mouse Otulin ELISA Kit

Mouse Otulin CLIA Kit

Mouse Otulin Protein

Mouse Otulin Polyclonal Antibody

Mouse Otulin Monoclonal Antibody

序列长度:

352

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

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350:

352:

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"The linear ubiquitin-specific deubiquitinase gumby regulates angiogenesis."

(PMID: 23708998)

[2].

"OTULIN antagonizes LUBAC signaling by specifically hydrolyzing Met1-linked polyubiquitin."

(PMID: 23746843)

[3].

"A Linear Diubiquitin-Based Probe for Efficient and Selective Detection of the Deubiquitinating Enzyme OTULIN."

(PMID: 28919039)

[4].

"CYLD Limits Lys63- and Met1-Linked Ubiquitin at Receptor Complexes to Regulate Innate Immune Signaling."

(PMID: 26997266)

[5].

"SPATA2 promotes CYLD activity and regulates TNF-induced NF-κB signaling and cell death."

(PMID: 27458237)

[6].

"The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity."

(PMID: 27523608)

[7].

"Biallelic hypomorphic mutations in a linear deubiquitinase define otulipenia, an early-onset autoinflammatory disease."

(PMID: 27559085)

[8].

"LUBAC-Recruited CYLD and A20 Regulate Gene Activation and Cell Death by Exerting Opposing Effects on Linear Ubiquitin in Signaling Complexes."

(PMID: 26670046)

[9].

"Molecular basis and regulation of OTULIN-LUBAC interaction."

(PMID: 24726323)

[10].

"Binding of OTULIN to the PUB domain of HOIP controls NF-κB signaling."

(PMID: 24726327)

[1].

人泛素thioesterase otulin(OTULIN)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人泛素thioesterase otulin(OTULIN)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人泛素thioesterase otulin(OTULIN)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人泛素thioesterase otulin(OTULIN)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人泛素thioesterase otulin(OTULIN)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人泛素thioesterase otulin(OTULIN)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人泛素thioesterase otulin(OTULIN)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人泛素thioesterase otulin(OTULIN)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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