PDX1 (基因名), Pancreas/duodenum homeobox protein 1 (蛋白名), pdx1_human.
产品名称:
Human PDX1/ Pancreas/duodenum homeobox protein 1 Recombinant Protein
胰十二指肠同源盒蛋白1
货号:
R9043h
商标:
EIAab®
监管等级:
别名:
Glucose-sensitive factor, Insulin promoter factor 1, Insulin upstream factor 1, Islet/duodenum homeobox-1, Somatostatin-transactivating factor 1, GSF, IPF-1, IUF-1, IDX-1, STF-1, PDX-1, IPF1, STF1
序列号:
P52945
来源:
E.coli
种属:
Human
标签:
His
序列:
Recombinant protein
纯度:
>90% by SDS-PAGE
浓度:
Reconstitution Dependent
形态:
Liquid
内毒素水平:
Please contact protein@eiaab.com The technician for more information.
应用:
存储缓冲液:
50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)
存储:
Store at -20°C. (Avoid repeated freezing and thawing.)
研究领域:
-
R&D 技术数据
更多信息,请参阅手册,或联系我们的技术支持: tech@eiaab.com.
基因位点
PDX1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
通用注释
亚单元:
Interacts with the basic helix-loop-helix domains of TCF3(E47) and NEUROD1 and with HMG-I(Y). Interacts with SPOP (By similarity). Interacts with the methyltransferase SETD7. Part of a PDX1:PBX1b:MEIS2b complex.
功能:
Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. As part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell.
亚细胞位置:
Nucleus
Cytoplasm
Cytosol
该产品尚未在任何出版物中被引用。
[1].
"Role of a proline insertion in the insulin promoter factor 1 (IPF1) gene in African Americans with type 2 diabetes."
[2].
"Missense mutations in the insulin promoter factor-1 gene predispose to type 2 diabetes."
[3].
"Defective mutations in the insulin promoter factor-1 (IPF-1) gene in late-onset type 2 diabetes mellitus."
[4].
"The p38/reactivating kinase mitogen-activated protein kinase cascade mediates the activation of the transcription factor insulin upstream factor 1 and insulin gene transcription by high glucose in pancreatic beta-cells."
[5].
"Isolation, characterization, and chromosomal mapping of the human insulin promoter factor 1 (IPF-1) gene."
[6].
"Localization of human homeodomain transcription factor insulin promoter factor 1 (IPF1) to chromosome band 13q12.1."
[7].
"Generation of homogeneous PDX1(+) pancreatic progenitors from human ES cell-derived endoderm cells."
[8].
"A novel hypomorphic PDX1 mutation responsible for permanent neonatal diabetes with subclinical exocrine deficiency."
[9].
"Association of a polymorphism of the apolipoprotein E gene with chronic kidney disease in Japanese individuals with metabolic syndrome."
[10].
"The expression pattern of PDX-1, SHH, Patched and Gli-1 is associated with pathological and clinical features in human pancreatic cancer."
[1].
人胰十二指肠同源盒蛋白1(PDX1)重组蛋白是否是无菌的?
蛋白试剂瓶和蛋白保存液是经过高压灭菌的,但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的,可以用0.2微米的滤器对蛋白进行过滤。
[2].
人胰十二指肠同源盒蛋白1(PDX1)重组蛋白的保存缓冲液是什么?
纯化后的蛋白保存在PBS(58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4)里,并往里面加入500mM咪唑和10%甘油。
[3].
怎样确定人胰十二指肠同源盒蛋白1(PDX1)重组蛋白的浓度?
蛋白浓度的确定没有一个统一的标准,这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。
[4].
人胰十二指肠同源盒蛋白1(PDX1)重组蛋白蛋白保存条件是怎样的?
蛋白应保存在 -20℃或 -80℃条件下,为了避免反复冻融,可以将蛋白分装成小份保存。
[5].
人胰十二指肠同源盒蛋白1(PDX1)重组蛋白是否可以用于活体实验?
重组蛋白没有用于任何的活体实验,因此蛋白的活性和半衰期是不确定的。
[6].
人胰十二指肠同源盒蛋白1(PDX1)重组蛋白的保质期是多久?
在适当的保存条件下,从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃,保证蛋白的保存浓度高于0.1mg/ml,限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题,请联系我们的技术支持。
[7].
你们蛋白和抗体的报价是怎么样的?
我们将根据你需要的蛋白和抗体的大小进行报价。
[8].
人胰十二指肠同源盒蛋白1(PDX1)重组蛋白是否能够提供蛋白片段?
我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分,我们将会按您的需求提供蛋白和抗体。
[9].
人胰十二指肠同源盒蛋白1(PDX1)重组蛋白的货期或发货时间一般是多长?
具体指标的货期需要确定。最快一周,最长可能一个月。
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