PIK3R6 (基因名), Phosphoinositide 3-kinase regulatory subunit 6 (蛋白名), pi3r6_human.
产品名称:
Human PIK3R6/ Phosphoinositide 3-kinase regulatory subunit 6 Recombinant Protein
货号:
R4039h
商标:
EIAab®
监管等级:
别名:
Phosphoinositide 3-kinase gamma adapter protein of 87 kDa, p84 PI3K adapter protein, p87 PI3K adapter protein, p84 PIKAP, p87PIKAP, C17orf38
序列号:
Q5UE93
来源:
E.coli
种属:
Human
标签:
His
序列:
1-363aa
预估分子量:
39.93 kDa (monomer)
纯度:
>90% by SDS-PAGE
浓度:
Reconstitution Dependent
形态:
Liquid
内毒素水平:
Please contact protein@eiaab.com The technician for more information.
应用:
存储缓冲液:
50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)
存储:
Store at -20°C. (Avoid repeated freezing and thawing.)
研究领域:
-
R&D 技术数据
Recombinant human PIK3R6 protein was determined by 12% SDS-PAGE stained with Coomassie Blue under reducing conditions.
基因位点
PIK3R6 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
通用注释
亚单元:
Heterodimer of a catalytic subunit (PIK3CG) and a regulatory (PIK3R6) subunit. The binding of PIK3R6 to PIK3CG may exclude the binding of PIK3R5 to PIK3CG. Interacts with beta-gamma G protein dimers. Interacts with PDE3B; the interaction allows recruitment of the PI3K gamma complex to a PDE3B:RAPGEF3 complex.
功能:
Regulatory subunit of the PI3K gamma complex. Acts as an adapter to drive activation of PIK3CG by beta-gamma G protein dimers. The PIK3CG:PIK3R6 heterodimer is much less sensitive to beta-gamma G protein dimers than PIK3CG:PIK3R5 and its membrane recruitment and beta-gamma G protein dimer-dependent activation requires HRAS bound to PIK3CG. Recruits of the PI3K gamma complex to a PDE3B:RAPGEF3 signaling complex involved in angiogenesis; signaling seems to involve RRAS.
亚细胞位置:
Cytoplasm
Cell membrane
Peripheral membrane protein
Translocated to the plasma membrane in a Ras-dependent manner.
验证序列:
该产品尚未在任何出版物中被引用。
[1].
"Characterization of p87PIKAP, a novel regulatory subunit of phosphoinositide 3-kinase gamma that is highly expressed in heart and interacts with PDE3B."
[2].
"A phosphodiesterase 3B-based signaling complex integrates exchange protein activated by cAMP 1 and phosphatidylinositol 3-kinase signals in human arterial endothelial cells."
[3].
"p84 forms a negative regulatory complex with p110γ to control PI3Kγ signalling during cell migration."
[4].
"p87 and p101 subunits are distinct regulators determining class IB phosphoinositide 3-kinase (PI3K) specificity."
[5].
"Differential roles for the p101 and p84 regulatory subunits of PI3Kγ in tumor growth and metastasis."
[6].
"GPCR activation of Ras and PI3Kc in neutrophils depends on PLCb2/b3 and the RasGEF RasGRP4."
[7].
"Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium."
[8].
"Phosphatidylinositol-3-kinase-gamma is integral to homing functions of progenitor cells."
[9].
"RACK1 regulates directional cell migration by acting on G betagamma at the interface with its effectors PLC beta and PI3K gamma."
[1].
人PIK3R6重组蛋白是否是无菌的？
蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。
[2].
人PIK3R6重组蛋白的保存缓冲液是什么？
纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。
[3].
怎样确定人PIK3R6重组蛋白的浓度？
蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。
[4].
人PIK3R6重组蛋白蛋白保存条件是怎样的？
蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。
[5].
人PIK3R6重组蛋白是否可以用于活体实验？
重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。
[6].
人PIK3R6重组蛋白的保质期是多久？
在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。
[7].
你们蛋白和抗体的报价是怎么样的？
我们将根据你需要的蛋白和抗体的大小进行报价。
[8].
人PIK3R6重组蛋白是否能够提供蛋白片段？
我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。
[9].
人PIK3R6重组蛋白的货期或发货时间一般是多长？
具体指标的货期需要确定。最快一周，最长可能一个月。
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