首页 > 重组蛋白 > Human PIK3CA Recombinant Protein

PIK3CA (基因名), Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (蛋白名), pk3ca_human.

产品名称:

Human PIK3CA/ Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform Recombinant Protein
磷脂酰肌醇-4,5-二磷酸3激酶催化剂α

货号:

R14307h

商标:

EIAab^®

监管等级:

RUO

别名:

Phosphatidylinositol 4, 5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha, Phosphoinositide-3-kinase catalytic alpha polypeptide, Serine/threonine protein kinase PIK3CA, PtdIns-3-kinase subunit p110-alpha, PI3-kinase subunit alpha

序列号:

P42336

来源:

E.coli

种属:

Human

标签:

His

序列:

520-730aa

预估分子量:

23.21 kDa (monomer)

纯度:

Greater than 95% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Immunology

规格 & 价格:

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Human PIK3CA Protein

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产品说明书

说明书:

MSDS:

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数据
文献
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

Recombinant human PIK3CA protein was determined by 12% SDS-PAGE stained with Coomassie Blue under reducing conditions.

基因位点

Cytogenetic band: 3q26.32 by HGNC 3q26.32 by Entrez Gene 3q26.32 by Ensembl

PIK3CA Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Heterodimer of a catalytic subunit PIK3CA and a p85 regulatory subunit (PIK3R1, PIK3R2 or PIK3R3) (PubMed:26593112). Interacts with IRS1 in nuclear extracts (By similarity). Interacts with RUFY3 (By similarity). Interacts with RASD2 (By similarity). Interacts with APPL1. Interacts with HRAS and KRAS (By similarity). Interaction with HRAS/KRAS is required for PI3K pathway signaling and cell proliferation stimulated by EGF and FGF2 (By similarity). Interacts with FAM83B; activates the PI3K/AKT signaling cascade (PubMed:23676467).

功能:

Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation through the PDPK1-AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. Also has serine-protein kinase activity: phosphorylates PIK3R1 (p85alpha regulatory subunit), EIF4EBP1 and HRAS. Plays a role in the positive regulation of phagocytosis and pinocytosis.

亚细胞位置:

N/A

数据库链接

UniGene:

Hs.553498

SMR:

P42336

STRING:

9606.ENSP00000263967

KEGG:

hsa:5290

MIM:

114480

Pfam:

PF00454

Uniprot:

P42336

相关产品

Mouse Pik3ca ELISA Kit

Bovine PIK3CA ELISA Kit

Mouse Pik3ca CLIA Kit

Bovine PIK3CA CLIA Kit

Mouse Pik3ca Protein

序列长度:

1068

序列:

仅供参考，详细请咨询（services@eiaab.com）

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1050:

1068:

WTTKMDWIFH | TIKQHALN

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"PIK3CA mutations mostly begin to develop in ductal carcinoma of the breast."

(PMID: 19818761)

[2].

"PIK3CA mutation associates with improved outcome in breast cancer."

(PMID: 19671852)

[3].

"Should individual PI3 kinase isoforms be targeted in cancer?"

(PMID: 19200708)

[4].

"PIK3CA mutation is associated with poor prognosis among patients with curatively resected colon cancer."

(PMID: 19237633)

[5].

"Genetic variations in the PI3K/PTEN/AKT/mTOR pathway are associated with clinical outcomes in esophageal cancer patients treated with chemoradiotherapy."

(PMID: 19164214)

[1].

人磷脂酰肌醇-4,5-二磷酸3激酶催化剂α(PIK3CA)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人磷脂酰肌醇-4,5-二磷酸3激酶催化剂α(PIK3CA)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人磷脂酰肌醇-4,5-二磷酸3激酶催化剂α(PIK3CA)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人磷脂酰肌醇-4,5-二磷酸3激酶催化剂α(PIK3CA)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人磷脂酰肌醇-4,5-二磷酸3激酶催化剂α(PIK3CA)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人磷脂酰肌醇-4,5-二磷酸3激酶催化剂α(PIK3CA)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人磷脂酰肌醇-4,5-二磷酸3激酶催化剂α(PIK3CA)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人磷脂酰肌醇-4,5-二磷酸3激酶催化剂α(PIK3CA)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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