首页 > 重组蛋白 > Human SLIT2 Recombinant Protein

SLIT2 (基因名), Slit homolog 2 protein (蛋白名), slit2_human.

产品名称:

Human SLIT2/ Slit homolog 2 protein Recombinant Protein
Slit-2

货号:

R0672h

商标:

EIAab^®

监管等级:

RUO

别名:

Slit-2, SLIL3

序列号:

O94813

来源:

E.coli

种属:

Human

标签:

His

序列:

1160-1333aa

预估分子量:

19.14 kDa (monomer)

纯度:

Greater than 90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

规格 & 价格:

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Human SLIT2 Protein

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产品说明书

说明书:

MSDS:

在线询价

数据
文献 ( 2 )
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

更多信息，请参阅手册，或联系我们的技术支持: tech@eiaab.com.

基因位点

Cytogenetic band: 4p15.31 by HGNC 4p15.31 by Entrez Gene 4p15.31 by Ensembl

SLIT2 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Interacts with GREM1 (By similarity). Homodimer. Binds ROBO1 and ROBO2 with high affinity.

功能:

Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth-stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal chord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration.

亚细胞位置:

Secreted The C-terminal cleavage protein is more diffusible than the larger N-terminal protein that is more tightly cell associated.

数据库链接

UniGene:

Hs.29802

SMR:

O94813

STRING:

9606.ENSP00000422591

KEGG:

hsa:9353

MIM:

603746

Pfam:

PF00008

Uniprot:

O94813

相关产品

Mouse Slit2 ELISA Kit

序列长度:

1529

序列:

仅供参考，详细请咨询（services@eiaab.com）

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KYSFECTDGS | SFVDEVEKVV | KCGCTRCVS

3D 模型:

3D 结构:

免疫原序列:

验证序列:

[1].

"SLIT2 attenuation during lung cancer progression deregulates β-catenin and E-cadherin and associates with poor prognosis"

RC Tseng, SH Lee, HS Hsu, et al.

From: Cancer research

[2].

"Slit2 and Robo1 induce opposing effects on metastasis of hepatocellular carcinoma Sk-hep-1 cells"

KHAN Y H, YE L, XIE F, et al.

From: International journal of oncology

[1].

"Mutations in the ROBO2 and SLIT2 genes are rare causes of familial vesico-ureteral reflux."

(PMID: 19350278)

[2].

"Structural insights into the Slit-Robo complex."

(PMID: 17848514)

[3].

"Frequent epigenetic inactivation of the SLIT2 gene in gliomas."

(PMID: 12881718)

[4].

"Slit2 is a repellent for retinal ganglion cell axons."

(PMID: 10864954)

[5].

"Cloning and expressions of three mammalian homologues of Drosophila slit suggest possible roles for Slit in the formation and maintenance of the nervous system."

(PMID: 9813312)

[1].

人Slit-2(SLIT2)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人Slit-2(SLIT2)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人Slit-2(SLIT2)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人Slit-2(SLIT2)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人Slit-2(SLIT2)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人Slit-2(SLIT2)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人Slit-2(SLIT2)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人Slit-2(SLIT2)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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