首页 > 重组蛋白 > Human SPHK2 Recombinant Protein

SPHK2 (基因名), Sphingosine kinase 2 (蛋白名), sphk2_human.

产品名称:

Human SPHK2/ Sphingosine kinase 2 Recombinant Protein
鞘氨醇激酶2

货号:

R0329h

商标:

EIAab^®

监管等级:

RUO

别名:

SK 2, SK2

序列号:

Q9NRA0

来源:

E.coli

种属:

Human

标签:

His

序列:

1-325aa

预估分子量:

35.75 kDa (monomer)

纯度:

Greater than 90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Cardiovascular

规格 & 价格:

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产品说明书

说明书:

MSDS:

在线询价

数据
文献
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

Recombinant human SPHK2 protein was determined by 12% SDS-PAGE stained with Coomassie Blue under reducing conditions.

基因位点

Cytogenetic band: 19q13.33 by HGNC 19q13.33 by Entrez Gene 19q13.33 by Ensembl

SPHK2 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Interacts with histone H3 (PubMed:19729656). Interacts with HDAC1, HDAC2, MBD2 and SIN3A (PubMed:19729656). Interacts with EEF1A1; the interaction enhances SPHK2 kinase activity (PubMed:18263879). Interacts with PHB2 (By similarity).

功能:

Catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine and L-threo-dihydrosphingosine. Binds phosphoinositides (PubMed:19168031, PubMed:12954646). In contrast to prosurvival SPHK1, has a positive effect on intracellular ceramide levels, inhibits cells growth and enhances apoptosis (PubMed:16118219). In mitochondria, is important for cytochrome-c oxidase assembly and mitochondrial respiration. The SPP produced in mitochondria binds PHB2 and modulates the regulation via PHB2 of complex IV assembly and respiration (PubMed:20959514). In nucleus, plays a role in epigenetic regulation of gene expression. Interacts with HDAC1 and HDAC2 and, through SPP production, inhibits their enzymatic activity, preventing the removal of acetyl groups from lysine residues with histones. Upregulates acetylation of histone H3-K9, histone H4-K5 and histone H2B-K12 (PubMed:19729656). In nucleus, may have an inhibitory effect on DNA synthesis and cell cycle (PubMed:12954646, PubMed:16103110). In mast cells, is the main regulator of SPP production which mediates calcium influx, NF-kappa-B activation, cytokine production, such as TNF and IL6, and degranulation of mast cells (By similarity). In dopaminergic neurons, is involved in promoting mitochondrial functions regulating ATP and ROS levels (By similarity). Also involved in the regulation of glucose and lipid metabolism (By similarity).

亚细胞位置:

Isoform 2 Lysosome membrane

数据库链接

UniGene:

Hs.528006

SMR:

Q9NRA0

STRING:

9606.ENSP00000245222

KEGG:

hsa:56848

MIM:

607092

Pfam:

PF00781

Uniprot:

Q9NRA0

相关产品

Mouse Sphk2 ELISA Kit

Mouse Sphk2 CLIA Kit

Mouse Sphk2 Protein

Mouse Sphk2 Polyclonal Antibody

Mouse Sphk2 Monoclonal Antibody

序列长度:

654

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

550:

600:

650:

664:

TLLTGPPGCP | GREP

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"A lipid binding domain in sphingosine kinase 2."

(PMID: 19168031)

[2].

"Molecular cloning and functional characterization of a novel mammalian sphingosine kinase type 2 isoform."

(PMID: 10751414)

[3].

"Cleavage of sphingosine kinase 2 by caspase-1 provokes its release from apoptotic cells."

(PMID: 20197547)

[4].

"Sphingosine kinase isoforms regulate oxaliplatin sensitivity of human colon cancer cells through ceramide accumulation and Akt activation."

(PMID: 19240026)

[5].

"Expression of sphingosine kinase 2 in synovial fibroblasts of rheumatoid arthritis contributing to apoptosis by a sphingosine analogue, FTY720."

(PMID: 19490468)

[6].

"Regulation of histone acetylation in the nucleus by sphingosine-1-phosphate."

(PMID: 19729656)

[7].

"Distinct roles of sphingosine kinases 1 and 2 in human mast-cell functions."

(PMID: 18178871)

[8].

"Recycling of sphingosine is regulated by the concerted actions of sphingosine-1-phosphate phosphohydrolase 1 and sphingosine kinase 2."

(PMID: 17895250)

[9].

"Sphingosine kinase type 2 activation by ERK-mediated phosphorylation."

(PMID: 17311928)

[10].

"Protein kinase D-mediated phosphorylation and nuclear export of sphingosine kinase 2."

(PMID: 17635916)

[1].

人鞘氨醇激酶2(SPHK2)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人鞘氨醇激酶2(SPHK2)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人鞘氨醇激酶2(SPHK2)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人鞘氨醇激酶2(SPHK2)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人鞘氨醇激酶2(SPHK2)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人鞘氨醇激酶2(SPHK2)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人鞘氨醇激酶2(SPHK2)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人鞘氨醇激酶2(SPHK2)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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