首页 > 重组蛋白 > Human SRSF1 Recombinant Protein

SRSF1 (基因名), Serine/arginine-rich splicing factor 1 (蛋白名), srsf1_human.

产品名称:

Human SRSF1/ Serine/arginine-rich splicing factor 1 Recombinant Protein
丝氨酸/精氨酸富有剪接因子1

货号:

R8282h

商标:

EIAab^®

监管等级:

RUO

别名:

Alternative-splicing factor 1, Splicing factor, arginine/serine-rich 1, pre-mRNA-splicing factor SF2, P33 subunit, ASF-1, OK/SW-cl.3, ASF, SF2, SF2P33, SFRS1

序列号:

Q07955

来源:

E.coli

种属:

Human

标签:

His

序列:

2-248aa

预估分子量:

27.17 kDa (monomer)

纯度:

>90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

规格 & 价格:

关注

Human SRSF1 Protein

邮箱 *

消息 *

Please 登录.

产品说明书

说明书:

MSDS:

在线询价

数据
文献 ( 1 )
出版物
序列 / 3D
常见问题解答
反馈墙 (0)

R&D 技术数据

更多信息，请参阅手册，或联系我们的技术支持: tech@eiaab.com.

基因位点

Cytogenetic band: 17q22 by HGNC 17q22 by Entrez Gene 17q22 by Ensembl

SRSF1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Consists of two polypeptides of p32 and p33. In vitro, self-associates and binds SRSF2, SNRNP70 and U2AF1 but not U2AF2. Binds SREK1/SFRS12. Interacts with SAFB/SAFB1. Interacts with PSIP1/LEDGF. Interacts with SRPK1. Identified in the spliceosome C complex. Interacts with RSRC1 (via Arg/Ser-rich domain). Interacts with ZRSR2/U2AF1-RS2. Interacts with CCDC55 (via C-terminus). Interacts with SRPK1 and a sliding docking interaction is essential for its sequential and processive phosphorylation by SRPK1. Interacts with NXF1. Interacts with CCNL1, CCNL2 and CDK11B (PubMed:18216018).

功能:

Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'-CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway.

亚细胞位置:

Cytoplasm Nucleus speckle In nuclear speckles. Shuttles between the nucleus and the cytoplasm.

数据库链接

UniGene:

Hs.68714

SMR:

Q07955

STRING:

9606.ENSP00000258962

KEGG:

hsa:6426

MIM:

600812

Pfam:

PF00076

Uniprot:

Q07955

相关产品

Mouse Srsf1 ELISA Kit

Bovine SRSF1 ELISA Kit

Chicken SRSF1 ELISA Kit

Pig SRSF1 ELISA Kit

Mouse Srsf1 CLIA Kit

序列长度:

248

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

248:

SYGRSRSRSR | SRSRSRSRSN | SRSRSYSPRR | SRGSPRYSPR | HSRSRSRT

3D 模型:

3D 结构:

免疫原序列:

验证序列:

[1].

"Salivary levels and immunohistochemical expression of selected angiogenic factors in benign and malignant parotid gland tumours"

J Sokalski, D Trzybulska, M Bodnar, et al.

From: Clinical Oral Investigations

[1].

"Chromosomal localization of mouse and human genes encoding the splicing factors ASF/SF2 (SFRS1) and SC-35 (SFRS2)."

(PMID: 8530103)

[2].

"Primary structure of the human splicing factor ASF reveals similarities with Drosophila regulators."

(PMID: 1855257)

[3].

"RRP1B is a metastasis modifier that regulates the expression of alternative mRNA isoforms through interactions with SRSF1."

(PMID: 23604122)

[4].

"NSrp70 is a novel nuclear speckle-related protein that modulates alternative pre-mRNA splicing in vivo."

(PMID: 21296756)

[5].

"A rational nomenclature for serine/arginine-rich protein splicing factors (SR proteins)."

(PMID: 20516191)

[6].

"Arginine methylation controls the subcellular localization and functions of the oncoprotein splicing factor SF2/ASF."

(PMID: 20308322)

[7].

"Role of cellular RNA processing factors in human immunodeficiency virus type 1 mRNA metabolism, replication, and infectivity."

(PMID: 19004959)

[8].

"Regiospecific phosphorylation control of the SR protein ASF/SF2 by SRPK1."

(PMID: 19477182)

[9].

"Lysine acetylation targets protein complexes and co-regulates major cellular functions."

(PMID: 19608861)

[10].

"Allosteric interactions direct binding and phosphorylation of ASF/SF2 by SRPK1."

(PMID: 19886675)

[1].

人丝氨酸/精氨酸富有剪接因子1(SRSF1)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人丝氨酸/精氨酸富有剪接因子1(SRSF1)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人丝氨酸/精氨酸富有剪接因子1(SRSF1)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人丝氨酸/精氨酸富有剪接因子1(SRSF1)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人丝氨酸/精氨酸富有剪接因子1(SRSF1)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人丝氨酸/精氨酸富有剪接因子1(SRSF1)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人丝氨酸/精氨酸富有剪接因子1(SRSF1)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人丝氨酸/精氨酸富有剪接因子1(SRSF1)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

反馈墙

评论数 : 0

所有用户