首页 > 重组蛋白 > Human TARDBP Recombinant Protein

TARDBP (基因名), TAR DNA-binding protein 43 (蛋白名), tadbp_human.

产品名称:

Human TARDBP/ TAR DNA-binding protein 43 Recombinant Protein
TARDNA结合蛋白43

货号:

R1951h

商标:

EIAab^®

监管等级:

RUO

别名:

TDP-43, TDP43

序列号:

Q13148

来源:

E.coli

种属:

Human

标签:

His

序列:

141-370aa

预估分子量:

25.3 kDa (monomer)

纯度:

Greater than 90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Neurosciences

规格 & 价格:

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产品说明书

说明书:

MSDS:

在线询价

数据
文献 ( 1 )
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

Recombinant human TARDBP protein was determined by 12% SDS-PAGE stained with Coomassie Blue under reducing conditions.

基因位点

Cytogenetic band: 1p36.22 by HGNC 1p36.22 by Entrez Gene 1p36.22 by Ensembl

TARDBP Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Homodimer. Interacts with BRDT (By similarity). Binds specifically to pyrimidine-rich motifs of TAR DNA and to single stranded TG repeated sequences. Binds to RNA, specifically to UG repeated sequences with a minimun of six contiguous repeats. Interacts with ATNX2; the interaction is RNA-dependent (PubMed:20740007). Interacts with MATR3 (PubMed:24686783). Interacts with UBQLN2 (PubMed:23541532). Interacts with HNRNPA2B1 (PubMed:19429692).

功能:

DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR.

亚细胞位置:

Nucleus In patients with frontotemporal lobar degeneration and amyotrophic lateral sclerosis, it is absent from the nucleus of affected neurons but it is the primary component of cytoplasmic ubiquitin-positive inclusion bodies.

数据库链接

UniGene:

Hs.300624

SMR:

Q13148

STRING:

9606.ENSP00000240185

KEGG:

hsa:23435

MIM:

605078

Pfam:

PF00076

Uniprot:

Q13148

相关产品

Mouse Tardbp ELISA Kit

Chicken TARDBP ELISA Kit

Mouse Tardbp CLIA Kit

Chicken TARDBP CLIA Kit

Mouse Tardbp Protein

序列长度:

414

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

250:

300:

350:

400:

414:

FGSSMDSKSS | GWGM

3D 模型:

3D 结构:

免疫原序列:

验证序列:

[1].

"TDP-43 as a possible biomarker for frontotemporal lobar degeneration: a systematic review of existing antibodies"

Goossens J, Vanmechelen E, Trojanowski J Q, et al.

From: Acta neuropathologica communications

[1].

"High frequency of TARDBP gene mutations in Italian patients with amyotrophic lateral sclerosis."

(PMID: 19224587)

[2].

"Cloning and characterization of a novel cellular protein, TDP-43, that binds to human immunodeficiency virus type 1 TAR DNA sequence motifs."

(PMID: 7745706)

[3].

"Association between novel TARDBP mutations and Chinese patients with amyotrophic lateral sclerosis."

(PMID: 20082726)

[4].

"TDP-43 dimerizes in human cells in culture."

(PMID: 20043239)

[5].

"Neuronal inclusion protein TDP-43 has no primary genetic role in FTD and ALS."

(PMID: 18068872)

[6].

"TARDBP mutations in motoneuron disease with frontotemporal lobar degeneration."

(PMID: 19350673)

[7].

"Genetic association analysis between TDP-43 polymorphisms and Alzheimer's disease in a Japanese population."

(PMID: 19851068)

[8].

"Screening for TARDBP mutations in Japanese familial amyotrophic lateral sclerosis."

(PMID: 19411082)

[9].

"TARDBP (TDP-43) sequence analysis in patients with familial and sporadic ALS: identification of two novel mutations."

(PMID: 19236453)

[10].

"TDP-43 A315T mutation in familial motor neuron disease."

(PMID: 18288693)

[1].

人TARDNA结合蛋白43(TARDBP)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人TARDNA结合蛋白43(TARDBP)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人TARDNA结合蛋白43(TARDBP)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人TARDNA结合蛋白43(TARDBP)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人TARDNA结合蛋白43(TARDBP)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人TARDNA结合蛋白43(TARDBP)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人TARDNA结合蛋白43(TARDBP)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人TARDNA结合蛋白43(TARDBP)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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