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Tet3 (基因名), Methylcytosine dioxygenase TET3 (蛋白名), tet3_mouse1.
产品名称:

Mouse Tet3/ Methylcytosine dioxygenase TET3 Recombinant Protein
Methylcytosine加双氧酶TET3

货号:

R7456m

商标:
EIAab®
监管等级:
序列号:
Q8BG87
来源:
E.coli
种属:
Mouse
标签:
His
序列:
1363-1523aa
预估分子量:
17.71 kDa (monomer)
纯度:
Greater than 90% by SDS-PAGE
浓度:
Reconstitution Dependent
形态:
Liquid
内毒素水平:
Please contact protein@eiaab.com The technician for more information.
应用:
存储缓冲液:
50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)
存储:
Store at -20°C. (Avoid repeated freezing and thawing.)
研究领域:
-
Mouse Tet3 Protein
规格 & 价格: cart
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Mouse Tet3 Protein
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产品说明书
说明书: 下载说明书
MSDS: MSDS
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R&D 技术数据
Mouse Tet3 Protein
Recombinant mouse Tet3 protein was determined by 12% SDS-PAGE stained with Coomassie Blue under reducing conditions.
通用注释


亚单元:
Interacts with HCFC1 and OGT.


功能:
Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in epigenetic chromatin reprogramming in the zygote following fertilization. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in DNA demethylation in the paternal pronucleus by mediating conversion of 5mC into 5hmC, 5fC and 5caC. Does not mediate DNA demethylation of maternal pronucleus because of the presence of DPPA3/PGC7 on maternal chromatin that prevents TET3-binding to chromatin. In addition to its role in DNA demethylation, also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT.


亚细胞位置:
Nucleus Cytoplasm At the zygotic stage, localizes in the male pronucleus, while it localizes to the cytoplasm at other preimplantation stages.


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