Zfp36l1 (基因名), mRNA decay activator protein ZFP36L1 (蛋白名), tisb_rat.
产品名称:
Rat Zfp36l1/ mRNA decay activator protein ZFP36L1 Recombinant Protein
mRNA衰变激活蛋白ZFP36L1
货号:
R5957r
商标:
EIAab®
监管等级:
别名:
Butyrate response factor 1, EGF-inducible protein CMG1, TPA-induced sequence 11b, Zinc finger protein 36, C3H1 type-like 1, ZFP36-like 1, Brf1, Cmg1, Tis11b
序列号:
P17431
来源:
E.coli
种属:
Rat
标签:
His
纯度:
>90% by SDS-PAGE
浓度:
Reconstitution Dependent
形态:
Liquid
内毒素水平:
Please contact protein@eiaab.com The technician for more information.
应用:
存储缓冲液:
50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)
存储:
Store at -20°C. (Avoid repeated freezing and thawing.)
研究领域:
Epigenetics
R&D 技术数据
更多信息,请参阅手册,或联系我们的技术支持: tech@eiaab.com.
通用注释
亚单元:
Associates with the cytoplasmic CCR4-NOT deadenylase and RNA exosome complexes to trigger ARE-containing mRNA deadenylation and decay processes. Interacts with CNOT1. Interacts (via N-terminus) with CNOT6. Interacts with CNOT7; this interaction is inhibited in response to phorbol 12-myristate 13-acetate (PMA) treatment in a p38 MAPK-dependent manner. Interacts with DCP1A. Interacts (via N-terminus) with DCP2. Interacts (via N-terminus) with EXOSC2. Interacts with XRN1. Interacts (via phosphorylated form) with YWHAB; this interaction occurs in a protein kinase AKT1-dependent manner. Interacts (via phosphorylated form) with YWHAZ; this interaction occurs in a p38 MAPK- and AKT-signaling pathways.
功能:
Zinc-finger RNA-binding protein that destabilizes several cytoplasmic AU-rich element (ARE)-containing mRNA transcripts by promoting their poly(A) tail removal or deadenylation, and hence provide a mechanism for attenuating protein synthesis (PubMed:10751406, PubMed:12748283). Acts as a 3'-untranslated region (UTR) ARE mRNA-binding adapter protein to communicate signaling events to the mRNA decay machinery (PubMed:12748283). Functions by recruiting the CCR4-NOT deadenylase complex and components of the cytoplasmic RNA decay machinery to the bound ARE-containing mRNAs, and hence promotes ARE-mediated mRNA deadenylation and decay processes (PubMed:12748283). Induces also the degradation of ARE-containing mRNAs even in absence of poly(A) tail (PubMed:11279239). Binds to 3'-UTR ARE of numerous mRNAs (PubMed:10751406). Positively regulates early adipogenesis by promoting ARE-mediated mRNA decay of immediate early genes (IEGs). Promotes ARE-mediated mRNA decay of mineralocorticoid receptor NR3C2 mRNA in response to hypertonic stress. Negatively regulates hematopoietic/erythroid cell differentiation by promoting ARE-mediated mRNA decay of the transcription factor STAT5B mRNA. Positively regulates monocyte/macrophage cell differentiation by promoting ARE-mediated mRNA decay of the cyclin-dependent kinase CDK6 mRNA. Promotes degradation of ARE-containing pluripotency-associated mRNAs in embryonic stem cells (ESCs), such as NANOG, through a fibroblast growth factor (FGF)-induced MAPK-dependent signaling pathway, and hence attenuates ESC self-renewal and positively regulates mesendoderm differentiation. May play a role in mediating pro-apoptotic effects in malignant B-cells by promoting ARE-mediated mRNA decay of BCL2 mRNA. In association with ZFP36L2 maintains quiescence on developing B lymphocytes by promoting ARE-mediated decay of several mRNAs encoding cell cycle regulators that help B cells progress through the cell cycle, and hence ensuring accurate variable-diversity-joining (VDJ) recombination and functional immune cell formation. Together with ZFP36L2 is also necessary for thymocyte development and prevention of T-cell acute lymphoblastic leukemia (T-ALL) transformation by promoting ARE-mediated mRNA decay of the oncogenic transcription factor NOTCH1 mRNA. Participates in the delivery of target ARE-mRNAs to processing bodies (PBs). In addition to its cytosolic mRNA-decay function, plays a role in the regulation of nuclear mRNA 3'-end processing; modulates mRNA 3'-end maturation efficiency of the DLL4 mRNA through binding with an ARE embedded in a weak noncanonical polyadenylation (poly(A)) signal in endothelial cells. Also involved in the regulation of stress granule (SG) and P-body (PB) formation and fusion. Plays a role in vasculogenesis and endocardial development. Plays a role in the regulation of keratinocyte proliferation, differentiation and apoptosis. Plays a role in myoblast cell differentiation.
亚细胞位置:
Nucleus
Cytoplasm
Cytoplasmic granule
Cytoplasm
P-body
Shuttles between the nucleus and the cytoplasm in a XPO1/CRM1-dependent manner. Component of cytoplasmic stress granules. Localizes in processing bodies (PBs).
该产品尚未在任何出版物中被引用。
[1].
大鼠mRNA衰变激活蛋白ZFP36L1(Zfp36l1)重组蛋白是否是无菌的?
蛋白试剂瓶和蛋白保存液是经过高压灭菌的,但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的,可以用0.2微米的滤器对蛋白进行过滤。
[2].
大鼠mRNA衰变激活蛋白ZFP36L1(Zfp36l1)重组蛋白的保存缓冲液是什么?
纯化后的蛋白保存在PBS(58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4)里,并往里面加入500mM咪唑和10%甘油。
[3].
怎样确定大鼠mRNA衰变激活蛋白ZFP36L1(Zfp36l1)重组蛋白的浓度?
蛋白浓度的确定没有一个统一的标准,这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。
[4].
大鼠mRNA衰变激活蛋白ZFP36L1(Zfp36l1)重组蛋白蛋白保存条件是怎样的?
蛋白应保存在 -20℃或 -80℃条件下,为了避免反复冻融,可以将蛋白分装成小份保存。
[5].
大鼠mRNA衰变激活蛋白ZFP36L1(Zfp36l1)重组蛋白是否可以用于活体实验?
重组蛋白没有用于任何的活体实验,因此蛋白的活性和半衰期是不确定的。
[6].
大鼠mRNA衰变激活蛋白ZFP36L1(Zfp36l1)重组蛋白的保质期是多久?
在适当的保存条件下,从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃,保证蛋白的保存浓度高于0.1mg/ml,限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题,请联系我们的技术支持。
[7].
你们蛋白和抗体的报价是怎么样的?
我们将根据你需要的蛋白和抗体的大小进行报价。
[8].
大鼠mRNA衰变激活蛋白ZFP36L1(Zfp36l1)重组蛋白是否能够提供蛋白片段?
我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分,我们将会按您的需求提供蛋白和抗体。
[9].
大鼠mRNA衰变激活蛋白ZFP36L1(Zfp36l1)重组蛋白的货期或发货时间一般是多长?
具体指标的货期需要确定。最快一周,最长可能一个月。
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