首页 > 重组蛋白 > Human TARBP2 Recombinant Protein

TARBP2 (基因名), RISC-loading complex subunit TARBP2 (蛋白名), trbp2_human.

产品名称:

Human TARBP2/ RISC-loading complex subunit TARBP2 Recombinant Protein
反式激活应答RNA结合蛋白

货号:

R3425h

商标:

EIAab^®

监管等级:

RUO

别名:

TAR RNA-binding protein 2, Trans-activation-responsive RNA-binding protein, TRBP

序列号:

Q15633

来源:

E.coli

种属:

Human

标签:

His

纯度:

>90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

规格 & 价格:

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Human TARBP2 Protein

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产品说明书

说明书:

MSDS:

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数据
文献
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

更多信息，请参阅手册，或联系我们的技术支持: tech@eiaab.com.

基因位点

Cytogenetic band: 12q13.13 by HGNC 12q13.13 by Entrez Gene 12q13.13 by Ensembl

TARBP2 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Self-associates. Component of the RISC loading complex (RLC), or micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2. Note that the trimeric RLC/miRLC is also referred to as RISC. Interacts with EIF2AK2/PKR and inhibits its protein kinase activity. Interacts with DHX9 and PRKRA. Interacts with DICER1, AGO2, MOV10, EIF6 and RPL7A (60S ribosome subunit); they form a large RNA-induced silencing complex (RISC) (PubMed:17507929).

功能:

Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. May also play a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1. Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs. This is achieved in part at least by binding to and inhibiting EIF2AK2/PKR, thereby reducing phosphorylation and inhibition of EIF2S1/eIF-2-alpha. May also promote translation of TAR-containing RNAs independently of EIF2AK2/PKR.

亚细胞位置:

Cytoplasm Cytoplasm Perinuclear region Nucleus

数据库链接

UniGene:

Hs.326

SMR:

Q15633

STRING:

9606.ENSP00000266987

KEGG:

hsa:6895

MIM:

605053

Pfam:

PF00035

Uniprot:

Q15633

相关产品

Mouse Tarbp2 ELISA Kit

Rat Tarbp2 ELISA Kit

Bovine TARBP2 ELISA Kit

Mouse Tarbp2 CLIA Kit

Rat Tarbp2 CLIA Kit

序列长度:

366

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

250:

300:

350:

366:

AARRALQYLK | IMAGSK

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"Characterization of a human TAR RNA-binding protein that activates the HIV-1 LTR."

(PMID: 2011739)

[2].

"The role of PACT in the RNA silencing pathway."

(PMID: 16424907)

[3].

"TRBP recruits the Dicer complex to Ago2 for microRNA processing and gene silencing."

(PMID: 15973356)

[4].

"A human, ATP-independent, RISC assembly machine fueled by pre-miRNA."

(PMID: 16357216)

[5].

"Organization of the human tarbp2 gene reveals two promoters that are repressed in an astrocytic cell line."

(PMID: 11641396)

[6].

"Two dimerization domains in the trans-activation response RNA-binding protein (TRBP) individually reverse the protein kinase R inhibition of HIV-1 long terminal repeat expression."

(PMID: 11438532)

[7].

"Genetic mapping in human and mouse of the locus encoding TRBP, a protein that binds the TAR region of the human immunodeficiency virus (HIV-1)."

(PMID: 7774957)

[8].

"Relatedness of an RNA-binding motif in human immunodeficiency virus type 1 TAR RNA-binding protein TRBP to human P1/dsI kinase and Drosophila staufen."

(PMID: 8455607)

[9].

"SUMOylation of TARBP2 regulates miRNA/siRNA efficiency."

(PMID: 26582366)

[10].

"Genetic variations in microRNA-related genes are associated with survival and recurrence in patients with renal cell carcinoma."

(PMID: 20732906)

[1].

人反式激活应答RNA结合蛋白(TARBP2)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人反式激活应答RNA结合蛋白(TARBP2)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人反式激活应答RNA结合蛋白(TARBP2)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人反式激活应答RNA结合蛋白(TARBP2)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人反式激活应答RNA结合蛋白(TARBP2)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人反式激活应答RNA结合蛋白(TARBP2)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人反式激活应答RNA结合蛋白(TARBP2)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人反式激活应答RNA结合蛋白(TARBP2)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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