首页 > 重组蛋白 > Human WAPL Recombinant Protein

WAPL (基因名), Wings apart-like protein homolog (蛋白名), wapl_human.

产品名称:

Human WAPL/ Wings apart-like protein homolog Recombinant Protein

货号:

商标:

EIAab^®

监管等级:

RUO

别名:

Friend of EBNA2 protein, WAPL cohesin release factor, FOE, KIAA0261, WAPAL

序列号:

Q7Z5K2

来源:

E.coli

种属:

Human

标签:

His

纯度:

>90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

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Human WAPL Protein

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产品说明书

说明书:

MSDS:

在线询价

数据
文献
出版物
序列 / 3D
常见问题解答
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基因位点

Cytogenetic band: 10q23.2 by HGNC 10q23.2 by Entrez Gene 10q23.2 by Ensembl

WAPL Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Interacts with the cohesin complex throughout the cell cycle; interacts with both chromatin-bound and soluble pools of the complex. Interacts with RAD21; the interaction is direct. Interacts with PDS5A; the interaction is direct, cohesin-dependent and competitive with CDCA5/SORORIN. Interacts (via FGF motifs) with PDS5B; the interaction is direct. Interacts with a SMC1 protein (SMC1A or SMC1B) and SMC3. Isoform 2 interacts with Epstein-Barr virus EBNA2.

功能:

Regulator of sister chromatid cohesion in mitosis which negatively regulates cohesin association with chromatin. Involved in both sister chromatid cohesion during interphase and sister-chromatid resolution during early stages of mitosis. Couples DNA replication to sister chromatid cohesion. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair.

亚细胞位置:

Nucleus Chromosome Cytoplasm Associates with chromatin through the cohesin complex during interphase. Released in the cytoplasm from nuclear envelope breakdown until anaphase, it reaccumulates in nucleus at telophase.

数据库链接

UniGene:

Hs.203099

SMR:

Q7Z5K2

STRING:

9606.ENSP00000298767

KEGG:

hsa:23063

MIM:

610754

Pfam:

PF07814

Uniprot:

Q7Z5K2

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序列长度:

1190

序列:

仅供参考，详细请咨询（services@eiaab.com）

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1190:

DFSIMTEMLK | KFLSFMNLTC | AVGTTGQKSI | SRVIEYLEHC |

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"Human Wapl is a cohesin-binding protein that promotes sister-chromatid resolution in mitotic prophase."

(PMID: 17112726)

[2].

"Identification and cloning of a novel chromatin-associated protein partner of Epstein-Barr nuclear protein 2."

(PMID: 15383329)

[3].

"Prediction of the coding sequences of unidentified human genes. VI. The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain."

(PMID: 9039502)

[4].

"Structure of the human cohesin inhibitor Wapl."

(PMID: 23776203)

[5].

"Sororin mediates sister chromatid cohesion by antagonizing Wapl."

(PMID: 21111234)

[6].

"Releasing cohesin from chromosome arms in early mitosis: opposing actions of Wapl-Pds5 and Sgo1."

(PMID: 19696148)

[7].

"Cohesin acetylation speeds the replication fork."

(PMID: 19907496)

[8].

"Expression of various types of alternatively spliced WAPL transcripts in human cervical epithelia."

(PMID: 18662753)

[9].

"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."

(PMID: 17081983)

[10].

"Wapl controls the dynamic association of cohesin with chromatin."

(PMID: 17113138)

[1].

人WAPL重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人WAPL重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人WAPL重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人WAPL重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人WAPL重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人WAPL重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人WAPL重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人WAPL重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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