首页 > 重组蛋白 > Human WTIP Recombinant Protein

WTIP (基因名), Wilms tumor protein 1-interacting protein (蛋白名), wtip_human.

产品名称:

Human WTIP/ Wilms tumor protein 1-interacting protein Recombinant Protein
肿瘤蛋白1相互作用的蛋白

货号:

R2052h

商标:

EIAab^®

监管等级:

RUO

别名:

WT1-interacting protein

序列号:

A6NIX2

来源:

E.coli

种属:

Human

标签:

His

纯度:

>90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

规格 & 价格:

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Human WTIP Protein

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产品说明书

说明书:

MSDS:

在线询价

数据
文献
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

更多信息，请参阅手册，或联系我们的技术支持: tech@eiaab.com.

基因位点

Cytogenetic band: 19q13.11 by HGNC 19q13.11 by Entrez Gene 19q13.11 by Ensembl

WTIP Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Forms homodimers (By similarity). Interacts with CD2AP and WT1. Interacts (via LIM domains) with SNAI1 (via SNAG domain), SNAI2/SLUG (via SNAG domain) and SCRT1 (via SNAG domain) (By similarity). Interacts with EIF4E, AGO1, AGO2, DCP2, DDX6, LATS1, LATS2, SAV1, EGLN2/PHD1 and EGLN3/PHD3. Interacts (via LIM domains) with isoform 1 and isoform 3 of VHL. Interacts with ROR2 (By similarity). Following treatment with bacterial lipopolysaccharide (LPS), forms a complex with MAPK8IP3 and dynein intermediate chain.

功能:

Adapter or scaffold protein which participates in the assembly of numerous protein complexes and is involved in several cellular processes such as cell fate determination, cytoskeletal organization, repression of gene transcription, cell-cell adhesion, cell differentiation, proliferation and migration. Positively regulates microRNA (miRNA)-mediated gene silencing. Negatively regulates Hippo signaling pathway and antagonizes phosphorylation of YAP1. Acts as a transcriptional corepressor for SNAI1 and SNAI2/SLUG-dependent repression of E-cadherin transcription. Acts as a hypoxic regulator by bridging an association between the prolyl hydroxylases and VHL enabling efficient degradation of HIF1A. In podocytes, may play a role in the regulation of actin dynamics and/or foot process cytoarchitecture (By similarity). In the course of podocyte injury, shuttles into the nucleus and acts as a transcription regulator that represses WT1-dependent transcription regulation, thereby translating changes in slit diaphragm structure into altered gene expression and a less differentiated phenotype.

亚细胞位置:

Cell junction Adherens junction Nucleus Cytoplasm P-body Following podocyte injury, caused by treatment with LPS, puromycin aminonucleoside, ultraviolet or hydrogen peroxide, translocates from sites of cell-cell contacts into the cytosol and nucleus. The shift from cell contacts to intracellular plaques starts as early as 1 hour after LPS stimulation and intranuclear localization begins 3 hours after LPS treatment. Maximal nuclear localization is achieved 6 hours after LPS treatment. Nuclear translocation requires dynein motor activity and intact microtubule network (By similarity). Returns to cell-cell contacts 24 hours after LPS stimulation. In the presence of ROR2, localizes to the plasma membrane (By similarity).

数据库链接

UniGene:

Hs.585010

SMR:

A6NIX2

STRING:

9606.ENSP00000270288

KEGG:

hsa:126374

MIM:

614790

Pfam:

PF00412

Uniprot:

A6NIX2

相关产品

Mouse Wtip ELISA Kit

Mouse Wtip CLIA Kit

Mouse Wtip Protein

Mouse Wtip Polyclonal Antibody

Mouse Wtip Monoclonal Antibody

序列长度:

430

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

250:

300:

350:

400:

430:

LAGHLLCRRC | HLRRLQPGPL | PSPTVHVTEL |

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"A WT1 co-regulator controls podocyte phenotype by shuttling between adhesion structures and nucleus."

(PMID: 14736876)

[2].

"The LIMD1 protein bridges an association between the prolyl hydroxylases and VHL to repress HIF-1 activity."

(PMID: 22286099)

[3].

"Identification and characterization of a set of conserved and new regulators of cytoskeletal organization, cell morphology and migration."

(PMID: 21834987)

[4].

"Podocyte injury induces nuclear translocation of WTIP via microtubule-dependent transport."

(PMID: 20086015)

[5].

"Ajuba LIM proteins are negative regulators of the Hippo signaling pathway."

(PMID: 20303269)

[6].

"LIM-domain proteins, LIMD1, Ajuba, and WTIP are required for microRNA-mediated gene silencing."

(PMID: 20616046)

[7].

"Proteome-wide analysis of phospho-regulated PDZ domain interactions."

(PMID: 30126976)

[8].

"Architecture of the human interactome defines protein communities and disease networks."

(PMID: 28514442)

[9].

"Nephrin Suppresses Hippo Signaling through the Adaptor Proteins Nck and WTIP."

(PMID: 27033705)

[10].

"WTIP interacts with BRCA2 and is essential for BRCA2 centrosome localization in cervical cancer cell."

(PMID: 27535760)

[1].

人肿瘤蛋白1相互作用的蛋白(WTIP)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人肿瘤蛋白1相互作用的蛋白(WTIP)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人肿瘤蛋白1相互作用的蛋白(WTIP)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人肿瘤蛋白1相互作用的蛋白(WTIP)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人肿瘤蛋白1相互作用的蛋白(WTIP)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人肿瘤蛋白1相互作用的蛋白(WTIP)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人肿瘤蛋白1相互作用的蛋白(WTIP)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人肿瘤蛋白1相互作用的蛋白(WTIP)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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