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HIF1A (基因名), Hypoxia-inducible factor 1-alpha (蛋白名), HIF1A_HUMAN1.

产品名称:

Human HIF1A/ Hypoxia-inducible factor 1-alpha ELISA Kit
缺氧诱导因子1-α

货号:

E0798h

商标:

EIAab^®

监管等级:

RUO

别名:

ARNT-interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, Class E basic helix-loop-helix protein 78, Member of PAS protein 1, Member of PAS protein 1, PAS domain-containing protein 8, PAS domain-containing protein 8, bHLHe78, bHLHe78, HIF-1-alpha, HIF-1-alpha, BHLHE78, BHLHE78, MOP1, MOP1, PASD8, PASD8

检测方法:

ELISA

特异性:

Natural and recombinant human Hypoxia-inducible factor 1-alpha

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

研究领域:

规格 & 价格:

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Human HIF1A ELISA Kit

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文献 ( 1 )
序列 / 3D
常见问题解答
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通用注释

亚单元:

Interacts with the ARNT; forms a heterodimer that binds core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (PubMed:10944113, PubMed:20699359). Interacts with COPS5; the interaction increases the transcriptional activity of HIF1A through increased stability (By similarity). Interacts with EP300 (via TAZ-type 1 domains); the interaction is stimulated in response to hypoxia and inhibited by CITED2. Interacts with CREBBP (via TAZ-type 1 domains). Interacts with NCOA1, NCOA2, APEX and HSP90. Interacts (hydroxylated within the ODD domain) with VHLL (via beta domain); the interaction, leads to polyubiquitination and subsequent HIF1A proteasomal degradation. During hypoxia, sumoylated HIF1A also binds VHL; the interaction promotes the ubiquitination of HIF1A. Interacts with SENP1; the interaction desumoylates HIF1A resulting in stabilization and activation of transcription. Interacts (Via the ODD domain) with ARD1A; the interaction appears not to acetylate HIF1A nor have any affect on protein stability, during hypoxia. Interacts with RWDD3; the interaction enhances HIF1A sumoylation. Interacts with TSGA10 (By similarity). Interacts with HIF3A (By similarity). Interacts with RORA (via the DNA binding domain); the interaction enhances HIF1A transcription under hypoxia through increasing protein stability. Interaction with PSMA7 inhibits the transactivation activity of HIF1A under both normoxic and hypoxia-mimicking conditions. Interacts with USP20. Interacts with RACK1; promotes HIF1A ubiquitination and proteasome-mediated degradation. Interacts (via N-terminus) with USP19. Interacts with SIRT2. Interacts (deacetylated form) with EGLN1. Interacts with CBFA2T3. Interacts with HSP90AA1 and HSP90AB1 (PubMed:26517842).

功能:

Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (By similarity). Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.

亚细胞位置:

Cytoplasm Nucleus Nucleus speckle Colocalizes with HIF3A in the nucleus and speckles (By similarity). Cytoplasmic in normoxia, nuclear translocation in response to hypoxia (PubMed:9822602).

数据库链接

UniGene:

Hs.597216

SMR:

Q16665

STRING:

9606.ENSP00000338018

KEGG:

hsa:3091

MIM:

603348

Pfam:

PF11413

UniGene:

Hs.597216

SMR:

Q16665

STRING:

9606.ENSP00000338018

KEGG:

hsa:3091

MIM:

603348

Pfam:

PF11413

Uniprot:

Q16665

相关产品

Mouse Hif1a ELISA Kit

Rat Hif1a ELISA Kit

Bovine HIF1A ELISA Kit

Chicken HIF1A ELISA Kit

Mouse Hif1a CLIA Kit

序列长度:

826

序列:

仅供参考，详细请咨询（services@eiaab.com）

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839:

DESGLPQLTS | YDCEVNAPIQ | GSRNLLQGEE | LLRALDQVN

3D 模型:

3D 结构:

免疫原序列:

验证序列:

[1].

"Characterization of Hypoxia-Inducible Factor-1α and Its Impact on Diagnosis and Prognosis of Hepatocellular Carcinoma in Hepatitis C Patients"

El-Deeb S O, Zied A B, Hendy O M, et al.

From: Journal of American Science

[1].

人缺氧诱导因子1-α(HIF1A)ELISA试剂盒可以做多少个样本？

人缺氧诱导因子1-α(HIF1A)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

人缺氧诱导因子1-α(HIF1A)ELISA试剂盒使用视频？

人缺氧诱导因子1-α(HIF1A)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

人缺氧诱导因子1-α(HIF1A)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的人缺氧诱导因子1-α(HIF1A)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

人缺氧诱导因子1-α(HIF1A)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

人缺氧诱导因子1-α(HIF1A)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析人缺氧诱导因子1-α(HIF1A)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

人缺氧诱导因子1-α(HIF1A)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集人缺氧诱导因子1-α(HIF1A)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

人缺氧诱导因子1-α(HIF1A)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

人缺氧诱导因子1-α(HIF1A)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

人缺氧诱导因子1-α(HIF1A)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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Human HIF1A/ Hypoxia-inducible factor 1-alpha ELISA Kit 缺氧诱导因子1-α

E0798h

Human HIF1A/ Hypoxia-inducible factor 1-alpha ELISA Kit
缺氧诱导因子1-α