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IL27 (基因名), Interleukin-27 subunit alpha (蛋白名), IL27A_HUMAN.

产品名称:

Human IL27/ Interleukin-27 subunit alpha ELISA Kit
白介素27α亚基

货号:

E0385h

商标:

EIAab^®

监管等级:

RUO

别名:

Interleukin-30, p28, IL-27 subunit alpha, IL27A, IL30

检测方法:

ELISA

实验类型:

Sandwich

检测范围:

15.6-1000pg/mL

灵敏度:

3.9pg/mL

特异性:

Natural and recombinant human Interleukin-27 subunit alpha

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

实验步骤:

研究领域:

Immunology

规格 & 价格:

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产品说明书

数据表:

说明书:

MSDS:

在线询价

数据
文献 ( 5 )
序列 / 3D
常见问题解答
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精密度

批内差：已知浓度的3个样本在一个板子内重复检测20次，以评估批内精密度。
批内 CV： ≤4.2%
批间差：已知浓度的3个样本在不同的板子上重复测定5次，以评估测定批间精密度。
批间 CV： ≤7.8%

回收率

回收率：低、中和高浓度的分析物被掺入到血清或者血浆样本中，进行回收实验测定。

Sample Type	Average(%)	Recovery Range(%)
Serum	103	97-109
Plasma	105	99-111

线性

线性：给定样本通过梯度稀释，每次稀释的测量值与理论值的比值。

Sample	1：2	1:4	1:8	1:16
serum(n=5)	93-93%	108-118%	99-108%	97-109%
EDTA plasma(n=5)	103-113%	99-108%	86-94%	107-119%
heparin plasma(n=5)	106-116%	104-113%	93-102%	111-120%

通用注释

亚单元:

Heterodimer with EBI3; not disulfide-linked. This heterodimer is known as interleukin IL-27.

功能:

Associates with EBI3 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR which appears to be required but not sufficient for IL-27-mediated signal transduction. IL-27 potentiate the early phase of TH1 response and suppress TH2 and TH17 differentiation. It induces the differentiation of TH1 cells via two distinct pathways, p38 MAPK/TBX21- and ICAM1/ITGAL/ERK-dependent pathways. It also induces STAT1, STAT3, STAT4 and STAT5 phosphorylation and activates TBX21/T-Bet via STAT1 with resulting IL12RB2 up-regulation, an event crucial to TH1 cell commitment. It suppresses the expression of GATA3, the inhibitor TH1 cells development. In CD8 T-cells, it activates STATs as well as GZMB. IL-27 reveals to be a potent inhibitor of TH17 cell development and of IL-17 production. Indeed IL27 alone is also able to inhibit the production of IL17 by CD4 and CD8 T-cells. While IL-27 suppressed the development of proinflammatory Th17 cells via STAT1, it inhibits the development of anti-inflammatory inducible regulatory T-cells, iTreg, independently of STAT1. IL-27 has also an effect on cytokine production, it suppresses proinflammatory cytokine production such as IL2, IL4, IL5 and IL6 and activates suppressors of cytokine signaling such as SOCS1 and SOCS3. Apart from suppression of cytokine production, IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T-cells. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines such as IP-10/CXCL10 and MIG/CXCL9. In vein endothelial cells, it induces IRF1/interferon regulatory factor 1 and increase the expression of MHC class II transactivator/CIITA with resulting up-regulation of major histocompatibility complex class II. IL-27 also demonstrates antiviral activity with inhibitory properties on HIV-1 replication.

亚细胞位置:

Secreted Does not seem to be secreted without coexpression of EBI3.

数据库链接

UniGene:

Hs.528111

STRING:

9606.ENSP00000349365

KEGG:

hsa:246778

MIM:

608273

Uniprot:

Q8NEV9

相关产品

序列长度:

243

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

243:

LSTYRLLHSL | ELVLSRAVRE | LLLLSKAGHS | VWPLGFPTLS | PQP

3D 模型:

3D 结构:

免疫原序列:

验证序列:

[1].

"Elevated interleukin-27 enhances the polarization of Th1/Tc1 cells and the production of proinflammatory cytokines in primary immune thrombocytopenia"

Hui Yuan Li , Dong Lei Zhang , Jing Ge , Hu Zhou , Ai ping Qi , Li Ma , Feng Xue , Ze Ping Zhou

From: Human Immunology

[2].

"Interleukin-27 enhances the production of tumour necrosis factor-α and interferon-γ by bone marrow T lymphocytes in aplastic anaemia"

JP Li, QJ Zhao, W Xing, et al.

From: British Journal of Haematology

[3].

"Increased EBI3 expression in placentas of preeclamptic patients"

M.L.P. Van Der Hoorn, R. Keijser, C. Ris-Stalpers, G. Afink, F.H.J. Claas, J.A.M. Van Der Post, S.A. Scherjon

From: Journal of Reproductive Immunology Volume 86, Issue 1 , Page 61, August 2010

[4].

"Expression levels of IL-27 and IL-17 in multiple myeloma patients: A higher ratio of IL-27:IL-17 in bone marrow was associated with a superior..."

XN Song, JZ Yang, LX Sun, et al.

From: Leukemia Research

[5].

"IL-27 在多发性骨髓瘤患者及骨髓瘤细胞系中的表达"

夏天, 李建平, 李文倩, et al.

From: 中华血液学杂志

[1].

人白介素27α亚基(IL27)ELISA试剂盒可以做多少个样本？

人白介素27α亚基(IL27)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

人白介素27α亚基(IL27)ELISA试剂盒使用视频？

人白介素27α亚基(IL27)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

人白介素27α亚基(IL27)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的人白介素27α亚基(IL27)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

人白介素27α亚基(IL27)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

人白介素27α亚基(IL27)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析人白介素27α亚基(IL27)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

人白介素27α亚基(IL27)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集人白介素27α亚基(IL27)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

人白介素27α亚基(IL27)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

人白介素27α亚基(IL27)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

人白介素27α亚基(IL27)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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Human IL27/ Interleukin-27 subunit alpha ELISA Kit 白介素27α亚基

E0385h

Interleukin-30, p28, IL-27 subunit alpha, IL27A, IL30

Human IL27/ Interleukin-27 subunit alpha ELISA Kit
白介素27α亚基