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CCND1 (基因名), G1/S-specific cyclin-D1 (蛋白名), ccnd1_human.

产品名称:

Human CCND1/ G1/S-specific cyclin-D1 ELISA Kit
G1/S-特异的细胞周期蛋白D1

货号:

E0585h

商标:

EIAab^®

监管等级:

RUO

别名:

B-cell lymphoma 1 protein, BCL-1 oncogene, PRAD1 oncogene, BCL-1, BCL1, PRAD1

检测方法:

ELISA

实验类型:

Sandwich

检测范围:

0.156-10ng/mL

灵敏度:

0.1ng/mL

特异性:

Natural and recombinant human G1/S-specific cyclin-D1

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

实验步骤:

研究领域:

Cancer

规格 & 价格:

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产品说明书

说明书:

MSDS:

在线询价

数据
文献 ( 3 )
序列 / 3D
常见问题解答
反馈墙 (0)

精密度

批内差：已知浓度的3个样本在一个板子内重复检测20次，以评估批内精密度。
批内 CV： ≤4.3%
批间差：已知浓度的3个样本在不同的板子上重复测定5次，以评估测定批间精密度。
批间 CV： ≤5.5%

回收率

回收率：低、中和高浓度的分析物被掺入到血清或者血浆样本中，进行回收实验测定。

Sample Type	Average(%)	Recovery Range(%)
Serum	86	80-92
Plasma	88	82-94

线性

线性：给定样本通过梯度稀释，每次稀释的测量值与理论值的比值。

Sample	1：2	1:4	1:8	1:16
serum(n=5)	85-95%	96-106%	95-105%	81-89%
EDTA plasma(n=5)	85-96%	81-93%	84-94%	115-125%
heparin plasma(n=5)	83-83%	107-117%	80-90%	108-118%

通用注释

亚单元:

Interacts with FBXO4 (By similarity). Interacts with either CDK4 or CDK6 protein kinase to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Component of the ternary complex CCND1/CDK4/CDKN1B required for nuclear translocation and modulation of CDK4-mediated kinase activity. Interacts directly with CDKN1B. Interacts with UHRF2; the interaction ubiquitinates CCND1 and appears to occur independently of phosphorylation. Can form similar complexes with either CDKN1A or CDKN2A. Interacts with USP2. Interacts (via cyclin N-terminal domain) with INSM1 (via N-terminal region); the interaction competes with the binding of CCND1 to CDK4 during cell cycle progression and inhibits CDK4 activity. Interacts with CDK4; the interaction is prevented with the binding of CCND1 to INSM1 during cell cycle progression.

功能:

Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner.

亚细胞位置:

Nucleus Cytoplasm Membrane Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members.

数据库链接

UniGene:

Hs.523852

SMR:

P24385

STRING:

9606.ENSP00000227507

KEGG:

hsa:595

MIM:

168461

Pfam:

PF02984

Uniprot:

P24385

相关产品

Mouse Ccnd1 ELISA Kit

Rat Ccnd1 ELISA Kit

Bovine CCND1 ELISA Kit

Chicken CCND1 ELISA Kit

Dog CCND1 ELISA Kit

序列长度:

295

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

250:

295:

IEALLESSLR | QAQQNMDPKA | AEEEEEEEEE | VDLACTPTDV | RDVDI

3D 模型:

3D 结构:

免疫原序列:

验证序列:

[1].

"Dual targeting of Notch and Wnt/β-catenin pathways: Potential approach in triple-negative breast cancer treatment"

F Nasser, N Moussa, M W. Helmy, et al.

From: Naunyn-Schmiedeberg's Archives of Pharmacology

[2].

"Epigenetic restoration and activation of ERβ: an inspiring approach for treatment of triple-negative breast cancer"

A Salahuddin, H Ghanem, G A. Omran, et al.

From: Medical Oncology

[3].

"CLM3, a multitarget tyrosine kinase inhibitor with antiangiogenic properties, is active against primary anaplastic thyroid cancer in vitro and in vivo"

A Antonelli, G Bocci, P Fallahi, et al.

From: The Journal of Clinical Endocrinology & Metabolism

[1].

人G1/S-特异的细胞周期蛋白D1(CCND1)ELISA试剂盒可以做多少个样本？

人G1/S-特异的细胞周期蛋白D1(CCND1)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

人G1/S-特异的细胞周期蛋白D1(CCND1)ELISA试剂盒使用视频？

人G1/S-特异的细胞周期蛋白D1(CCND1)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

人G1/S-特异的细胞周期蛋白D1(CCND1)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的人G1/S-特异的细胞周期蛋白D1(CCND1)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

人G1/S-特异的细胞周期蛋白D1(CCND1)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

人G1/S-特异的细胞周期蛋白D1(CCND1)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析人G1/S-特异的细胞周期蛋白D1(CCND1)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

人G1/S-特异的细胞周期蛋白D1(CCND1)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集人G1/S-特异的细胞周期蛋白D1(CCND1)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

人G1/S-特异的细胞周期蛋白D1(CCND1)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

人G1/S-特异的细胞周期蛋白D1(CCND1)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

人G1/S-特异的细胞周期蛋白D1(CCND1)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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Human CCND1/ G1/S-specific cyclin-D1 ELISA Kit G1/S-特异的细胞周期蛋白D1

E0585h

B-cell lymphoma 1 protein, BCL-1 oncogene, PRAD1 oncogene, BCL-1, BCL1, PRAD1

Human CCND1/ G1/S-specific cyclin-D1 ELISA Kit
G1/S-特异的细胞周期蛋白D1