首页 > 重组蛋白 > Human ILK Recombinant Protein

ILK (基因名), Integrin-linked protein kinase (蛋白名), ilk_human.

产品名称:

Human ILK/ Integrin-linked protein kinase Recombinant Protein
整合素连接蛋白激酶

货号:

R1587h

商标:

EIAab^®

监管等级:

RUO

别名:

59 kDa serine/threonine-protein kinase, ILK-1, ILK-2, p59ILK, ILK1, ILK2

序列号:

Q13418

来源:

E.coli

种属:

Human

标签:

His

纯度:

>90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Cancer

规格 & 价格:

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Human ILK Protein

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产品说明书

说明书:

MSDS:

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数据
文献
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

更多信息，请参阅手册，或联系我们的技术支持: tech@eiaab.com.

基因位点

Cytogenetic band: 11p15.4 by HGNC 11p15.4 by Entrez Gene 11p15.4 by Ensembl

ILK Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Interacts with FERMT2 (By similarity). Interacts with the cytoplasmic domain of ITGB1. Could also interact with integrin ITGB2, ITGB3 and/or ITGB5. Interacts (via ANK repeats) with LIMS1 and LIMS2. Interacts with PARVA and PARVB; these compete for the same binding site. Interacts probably also with TGFB1I1. Interacts (via ANK repeats) with EPHA1 (via SAM domain); stimulated by EFNA1 but independent of the kinase activity of EPHA1. Interacts with LIMD2; leading to activate the protein kinase activity (PubMed:24590809).

功能:

Receptor-proximal protein kinase regulating integrin-mediated signal transduction (PubMed:8538749, PubMed:9736715). May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B (PubMed:8538749, PubMed:9736715).

亚细胞位置:

Cell junction Focal adhesion Cell membrane Peripheral membrane protein Cytoplasmic side Cell projection Lamellipodium Cytoplasm Myofibril Sarcomere

数据库链接

UniGene:

Hs.706355

SMR:

Q13418

STRING:

9606.ENSP00000299421

KEGG:

hsa:3611

MIM:

602366

Pfam:

PF12796

Uniprot:

Q13418

相关产品

序列长度:

452

序列:

仅供参考，详细请咨询（services@eiaab.com）

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3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"Cloning of an isoform of integrin-linked kinase (ILK) that is upregulated in HT-144 melanoma cells following TGF-beta1 stimulation."

(PMID: 10871859)

[2].

"Phosphoinositide-3-OH kinase-dependent regulation of glycogen synthase kinase 3 and protein kinase B/AKT by the integrin-linked kinase."

(PMID: 9736715)

[3].

"Regulation of cell adhesion and anchorage-dependent growth by a new beta 1-integrin-linked protein kinase."

(PMID: 8538749)

[4].

"Tumor expression of integrin-linked kinase (ILK) correlates with the expression of the E-cadherin repressor snail: an immunohistochemical study in ductal pancreatic adenocarcinoma."

(PMID: 20091050)

[5].

"Hypoxia induces BMP-2 expression via ILK, Akt, mTOR, and HIF-1 pathways in osteoblasts."

(PMID: 20232298)

[6].

"Integrin-linked kinase: a multi-functional regulator modulating extracellular pressure-stimulated cancer cell adhesion through focal adhesion kinase and AKT."

(PMID: 19633364)

[7].

"EphA1 interacts with integrin-linked kinase and regulates cell morphology and motility."

(PMID: 19118217)

[8].

"Structural basis of focal adhesion localization of LIM-only adaptor PINCH by integrin-linked kinase."

(PMID: 19117955)

[9].

"[Relationship between integrin-linked kinase expression and renal glomerular damage in children with Henoch-Schnlein purpura nephritis]."

(PMID: 20113654)

[10].

"The pseudoactive site of ILK is essential for its binding to alpha-Parvin and localization to focal adhesions."

(PMID: 20005845)

[1].

人整合素连接蛋白激酶(ILK)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人整合素连接蛋白激酶(ILK)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人整合素连接蛋白激酶(ILK)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人整合素连接蛋白激酶(ILK)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人整合素连接蛋白激酶(ILK)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人整合素连接蛋白激酶(ILK)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人整合素连接蛋白激酶(ILK)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人整合素连接蛋白激酶(ILK)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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