首页 > 重组蛋白 > Human ALOX15 Recombinant Protein

ALOX15 (基因名), Arachidonate 15-lipoxygenase (蛋白名), lox15_human.

产品名称:

Human ALOX15/ Arachidonate 15-lipoxygenase Recombinant Protein
花生四烯酸15-lipoxygenase

货号:

R0891h

商标:

EIAab^®

监管等级:

RUO

别名:

12/15-lipoxygenase, Arachidonate 12-lipoxygenase, leukocyte-type, Arachidonate omega-6 lipoxygenase, 12-LOX, 15-LOX, LOG15

序列号:

P16050

来源:

E.coli

种属:

Human

标签:

His

纯度:

>90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Cardiovascular

规格 & 价格:

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Human ALOX15 Protein

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产品说明书

说明书:

MSDS:

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数据
文献
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

更多信息，请参阅手册，或联系我们的技术支持: tech@eiaab.com.

基因位点

Cytogenetic band: 17p13.2 by HGNC 17p13.2 by Entrez Gene 17p13.2 by Ensembl

ALOX15 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Interacts with PEBP1; in response to IL13/interleukin-13, prevents the interaction of PEBP1 with RAF1 to activate the ERK signaling cascade.

功能:

Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Converts arachidonic acid into 12-hydroperoxyeicosatetraenoic acid/12-HPETE and 15-hydroperoxyeicosatetraenoic acid/15-HPETE. Also converts linoleic acid to 13-hydroperoxyoctadecadienoic acid. May also act on (12S)-hydroperoxyeicosatetraenoic acid/(12S)-HPETE to produce hepoxilin A3. Probably plays an important role in the immune and inflammatory responses. Through the oxygenation of membrane-bound phosphatidylethanolamine in macrophages may favor clearance of apoptotic cells during inflammation by resident macrophages and prevent an autoimmune response associated with the clearance of apoptotic cells by inflammatory monocytes. In parallel, may regulate actin polymerization which is crucial for several biological processes, including macrophage function. May also regulate macrophage function through regulation of the peroxisome proliferator activated receptor signaling pathway. Finally, it is also involved in the cellular response to IL13/interleukin-13. In addition to its role in the immune and inflammatory responses, may play a role in epithelial wound healing in the cornea maybe through production of lipoxin A4. May also play a role in endoplasmic reticulum stress response and the regulation of bone mass.

亚细胞位置:

Cytoplasm Cytosol Cell membrane Peripheral membrane protein Lipid droplet Predominantly cytosolic; becomes enriched at membranes upon calcium binding. Translocates from the cytosol to the plasma membrane when stimulated by IL13/interleukin-13 and in macrophages binding apoptotic cells.

数据库链接

UniGene:

Hs.73809

SMR:

P16050

STRING:

9606.ENSP00000293761

KEGG:

hsa:246

MIM:

152392

Pfam:

PF00305

Uniprot:

P16050

相关产品

Human ALOX15 ELISA Kit

Human ALOX15 CLIA Kit

Human ALOX15 Polyclonal Antibody

Human ALOX15 Monoclonal Antibody

Human ALOX15

序列长度:

662

序列:

仅供参考，详细请咨询（services@eiaab.com）

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662:

LRPSVVENSV | AI

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"A near null variant of 12/15-LOX encoded by a novel SNP in ALOX15 and the risk of coronary artery disease."

(PMID: 17959182)

[2].

"Variation in eicosanoid genes, non-fatal myocardial infarction and ischemic stroke."

(PMID: 19046748)

[3].

"Reciprocal regulation of 12- and 15-lipoxygenases by UV-irradiation in human keratinocytes."

(PMID: 18755188)

[4].

"15-Lipoxygenase gene variants are associated with carotid plaque but not carotid intima-media thickness."

(PMID: 18392641)

[5].

"The c.-292C>T promoter polymorphism increases reticulocyte-type 15-lipoxygenase-1 activity and could be atheroprotective."

(PMID: 17439326)

[6].

"Polymorphisms in ALOX12, but not ALOX15, are significantly associated with BMD in postmenopausal women."

(PMID: 17520163)

[7].

"Human ALOX12, but not ALOX15, is associated with BMD in white men and women."

(PMID: 16598376)

[8].

"Association of a single nucleotide polymorphism in the lipoxygenase ALOX15 5'-flanking region (-5229G/A) with bone mineral density."

(PMID: 15838625)

[9].

"Purification of two forms of arachidonate 15-lipoxygenase from human leukocytes."

(PMID: 1662607)

[10].

"15-Lipoxygenase 1 interacts with phosphatidylethanolamine-binding protein to regulate MAPK signaling in human airway epithelial cells."

(PMID: 21831839)

[1].

人花生四烯酸15-lipoxygenase(ALOX15)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人花生四烯酸15-lipoxygenase(ALOX15)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人花生四烯酸15-lipoxygenase(ALOX15)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人花生四烯酸15-lipoxygenase(ALOX15)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人花生四烯酸15-lipoxygenase(ALOX15)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人花生四烯酸15-lipoxygenase(ALOX15)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人花生四烯酸15-lipoxygenase(ALOX15)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人花生四烯酸15-lipoxygenase(ALOX15)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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