首页 > 重组蛋白 > Human NANOG Recombinant Protein

NANOG (基因名), Homeobox protein NANOG (蛋白名), nanog_human.

产品名称:

Human NANOG/ Homeobox protein NANOG Recombinant Protein
同源异形盒蛋白NANOG

货号:

R14436h

商标:

EIAab^®

监管等级:

RUO

别名:

Homeobox transcription factor Nanog, hNanog

序列号:

Q9H9S0

来源:

E.coli

种属:

Human

标签:

His

纯度:

>90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

规格 & 价格:

关注

Human NANOG Protein

邮箱 *

消息 *

Please 登录.

产品说明书

说明书:

MSDS:

在线询价

数据
文献
出版物
序列 / 3D
常见问题解答
反馈墙 (0)

R&D 技术数据

更多信息，请参阅手册，或联系我们的技术支持: tech@eiaab.com.

基因位点

Cytogenetic band: 12p13.31 by HGNC 12p13.31 by Entrez Gene 12p13.31 by Ensembl

NANOG Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Interacts with SMAD1 and SALL4. Interacts with ZNF281/ZFP281 (By similarity). Interacts with PCGF1 (PubMed:26687479).

功能:

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.

亚细胞位置:

Nucleus

数据库链接

UniGene:

Hs.635882

SMR:

Q9H9S0

STRING:

9606.ENSP00000229307

KEGG:

hsa:79923

MIM:

607937

Pfam:

PF00046

Uniprot:

Q9H9S0

相关产品

Mouse Nanog ELISA Kit

Bovine NANOG ELISA Kit

Mouse Nanog CLIA Kit

Bovine NANOG CLIA Kit

Mouse Nanog Protein

序列长度:

305

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

250:

300:

305:

QPEDV

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"Downregulation of NANOG induces differentiation of human embryonic stem cells to extraembryonic lineages."

(PMID: 15983365)

[2].

"Human STELLAR, NANOG, and GDF3 genes are expressed in pluripotent cells and map to chromosome 12p13, a hotspot for teratocarcinoma."

(PMID: 14990856)

[3].

"Identification, cloning and expression analysis of the pluripotency promoting Nanog genes in mouse and human."

(PMID: 15108323)

[4].

"The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells."

(PMID: 12787504)

[5].

"Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells."

(PMID: 12787505)

[6].

"The variant Polycomb Repressor Complex 1 component PCGF1 interacts with a pluripotency sub-network that includes DPPA4, a regulator of embryogenesis."

(PMID: 26687479)

[7].

"Structure-based discovery of NANOG variant with enhanced properties to promote self-renewal and reprogramming of pluripotent stem cells."

(PMID: 25825768)

[8].

"Positive correlations of Oct-4 and Nanog in oral cancer stem-like cells and high-grade oral squamous cell carcinoma."

(PMID: 18593985)

[9].

"Pluripotential competence of cells associated with Nanog activity."

(PMID: 15582778)

[10].

"Sequential DNA methylation of the Nanog and Oct-4 upstream regions in human NT2 cells during neuronal differentiation."

(PMID: 15615706)

[1].

人同源异形盒蛋白NANOG(NANOG)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人同源异形盒蛋白NANOG(NANOG)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人同源异形盒蛋白NANOG(NANOG)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人同源异形盒蛋白NANOG(NANOG)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人同源异形盒蛋白NANOG(NANOG)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人同源异形盒蛋白NANOG(NANOG)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人同源异形盒蛋白NANOG(NANOG)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人同源异形盒蛋白NANOG(NANOG)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

反馈墙

评论数 : 0

所有用户