首页 > 重组蛋白 > Human SIRT5 Recombinant Protein

SIRT5 (基因名), NAD-dependent protein deacylase sirtuin-5, mitochondrial (蛋白名), sir5_human.

产品名称:

Human SIRT5/ NAD-dependent protein deacylase sirtuin-5, mitochondrial Recombinant Protein
沉默调节蛋白5

货号:

R11384h

商标:

EIAab^®

监管等级:

RUO

别名:

Regulatory protein SIR2 homolog 5, SIR2-like protein 5, SIR2L5

序列号:

Q9NXA8

来源:

E.coli

种属:

Human

标签:

His

纯度:

>90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

规格 & 价格:

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Human SIRT5 Protein

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产品说明书

说明书:

MSDS:

在线询价

数据
文献
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

更多信息，请参阅手册，或联系我们的技术支持: tech@eiaab.com.

基因位点

Cytogenetic band: 6p23 by HGNC 6p23 by Entrez Gene 6p23 by Ensembl

SIRT5 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Interacts with CPS1 (By similarity). Monomer. Homodimer. Forms homodimers upon suramin binding.

功能:

NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins (PubMed:21908771, PubMed:22076378, PubMed:24703693). Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting (PubMed:22076378, PubMed:24703693). Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species (PubMed:24140062). Modulates ketogenesis through the desuccinylation and activation of HMGCS2 (By similarity). Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro such as UOX.

亚细胞位置:

Isoform 2 Mitochondrion

数据库链接

UniGene:

Hs.567431

SMR:

Q9NXA8

STRING:

9606.ENSP00000368552

KEGG:

hsa:23408

MIM:

604483

Pfam:

PF02146

Uniprot:

Q9NXA8

相关产品

Mouse Sirt5 ELISA Kit

Rat Sirt5 ELISA Kit

Bovine SIRT5 ELISA Kit

Chicken SIRT5 ELISA Kit

Dog SIRT5 ELISA Kit

序列长度:

310

序列:

仅供参考，详细请咨询（services@eiaab.com）

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310:

LACHENETVS |

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"Substrates and regulation mechanisms for the human mitochondrial sirtuins Sirt3 and Sirt5."

(PMID: 18680753)

[2].

"Characterization of five human cDNAs with homology to the yeast SIR2 gene: Sir2-like proteins (sirtuins) metabolize NAD and may have protein ADP-ribosyltransferase activity."

(PMID: 10381378)

[3].

"SHMT2 Desuccinylation by SIRT5 Drives Cancer Cell Proliferation."

(PMID: 29180469)

[4].

"Lysine glutarylation is a protein posttranslational modification regulated by SIRT5."

(PMID: 24703693)

[5].

"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."

(PMID: 23806337)

[6].

"SIRT5 desuccinylates and activates SOD1 to eliminate ROS."

(PMID: 24140062)

[7].

"The bicyclic intermediate structure provides insights into the desuccinylation mechanism of human sirtuin 5 (SIRT5)."

(PMID: 22767592)

[8].

"Sirt5 deacylation activities show differential sensitivities to nicotinamide inhibition."

(PMID: 23028781)

[9].

"Distinct regulation of mitochondrial localization and stability of two human Sirt5 isoforms."

(PMID: 21143562)

[10].

"The first identification of lysine malonylation substrates and its regulatory enzyme."

(PMID: 21908771)

[1].

人沉默调节蛋白5(SIRT5)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人沉默调节蛋白5(SIRT5)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人沉默调节蛋白5(SIRT5)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人沉默调节蛋白5(SIRT5)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人沉默调节蛋白5(SIRT5)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人沉默调节蛋白5(SIRT5)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人沉默调节蛋白5(SIRT5)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人沉默调节蛋白5(SIRT5)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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