首页 > 重组蛋白 > Human SMAD7 Recombinant Protein

SMAD7 (基因名), Mothers against decapentaplegic homolog 7 (蛋白名), smad7_human.

产品名称:

Human SMAD7/ Mothers against decapentaplegic homolog 7 Recombinant Protein
Smad7

货号:

R0648h

商标:

EIAab^®

监管等级:

RUO

别名:

Mothers against decapentaplegic homolog 8, SMAD family member 7, MAD homolog 8, SMAD 7, MAD homolog 7, MADH7, MADH8

序列号:

O15105

来源:

E.coli

种属:

Human

标签:

His

纯度:

>90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

Epigenetics

规格 & 价格:

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Human SMAD7 Protein

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产品说明书

说明书:

MSDS:

在线询价

数据
文献
出版物
序列 / 3D
常见问题解答
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R&D 技术数据

更多信息，请参阅手册，或联系我们的技术支持: tech@eiaab.com.

基因位点

Cytogenetic band: 18q21.1 by HGNC 18q21.1 by Entrez Gene 18q21.1 by Ensembl

SMAD7 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Interacts with WWP1 (By similarity). Interacts with COPS5. Interacts with NEDD4L. Interacts with STAMBP. Interacts with RNF111, AXIN1 and AXIN2. Interacts with PPP1R15A. Interacts (via MH2 domain) with EP300. Interacts with ACVR1B, SMURF1, SMURF2 and TGFBR1; SMAD7 recruits SMURF1 and SMURF2 to the TGF-beta receptor and regulates its degradation. Interacts with PDPK1 (via PH domain).

功能:

Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

亚细胞位置:

Nucleus Cytoplasm Interaction with NEDD4L or RNF111 induces translocation from the nucleus to the cytoplasm (PubMed:16601693). TGF-beta stimulates its translocation from the nucleus to the cytoplasm. PDPK1 inhibits its translocation from the nucleus to the cytoplasm in response to TGF-beta (PubMed:17327236).

数据库链接

UniGene:

Hs.465087

SMR:

O15105

STRING:

9606.ENSP00000262158

KEGG:

hsa:4092

MIM:

602932

Pfam:

PF03165

Uniprot:

O15105

相关产品

Mouse Smad7 ELISA Kit

序列长度:

426

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

250:

300:

350:

400:

426:

KGWGQCYTRQ | FISSCPCWLE | VIFNSR

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"Increased risk of colon cancer associated with a genetic polymorphism of SMAD7."

(PMID: 20124488)

[2].

"A genome-wide association study shows that common alleles of SMAD7 influence colorectal cancer risk."

(PMID: 17934461)

[3].

"Axin is a scaffold protein in TGF-beta signaling that promotes degradation of Smad7 by Arkadia."

(PMID: 16601693)

[4].

"Jab1/CSN5, a component of the COP9 signalosome, regulates transforming growth factor beta signaling by binding to Smad7 and promoting its degradation."

(PMID: 14993265)

[5].

"GADD34-PP1c recruited by Smad7 dephosphorylates TGFbeta type I receptor."

(PMID: 14718519)

[6].

"Arkadia amplifies TGF-beta superfamily signalling through degradation of Smad7."

(PMID: 14657019)

[7].

"Smurf1 interacts with transforming growth factor-beta type I receptor through Smad7 and induces receptor degradation."

(PMID: 11278251)

[8].

"Smad7 binds to Smurf2 to form an E3 ubiquitin ligase that targets the TGF beta receptor for degradation."

(PMID: 11163210)

[9].

"Vascular MADs: two novel MAD-related genes selectively inducible by flow in human vascular endothelium."

(PMID: 9256479)

[10].

"Identification of Smad7, a TGFbeta-inducible antagonist of TGF-beta signalling."

(PMID: 9335507)

[1].

人Smad7(SMAD7)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人Smad7(SMAD7)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人Smad7(SMAD7)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人Smad7(SMAD7)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人Smad7(SMAD7)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人Smad7(SMAD7)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人Smad7(SMAD7)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人Smad7(SMAD7)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

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