首页 > 重组蛋白 > Human SMURF2 Recombinant Protein

SMURF2 (基因名), E3 ubiquitin-protein ligase SMURF2 (蛋白名), smuf2_human.

产品名称:

Human SMURF2/ E3 ubiquitin-protein ligase SMURF2 Recombinant Protein
E3泛素蛋白连接酶Smurf2

货号:

R2373h

商标:

EIAab^®

监管等级:

RUO

别名:

HECT-type E3 ubiquitin transferase SMURF2, SMAD ubiquitination regulatory factor 2, SMAD-specific E3 ubiquitin-protein ligase 2, hSMURF2

序列号:

Q9HAU4

来源:

E.coli

种属:

Human

标签:

His

纯度:

>90% by SDS-PAGE

浓度:

Reconstitution Dependent

形态:

Liquid

内毒素水平:

Please contact protein@eiaab.com The technician for more information.

应用:

存储缓冲液:

50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)

存储:

Store at -20°C. (Avoid repeated freezing and thawing.)

研究领域:

规格 & 价格:

关注

Human SMURF2 Protein

邮箱 *

消息 *

Please 登录.

产品说明书

说明书:

MSDS:

在线询价

数据
文献
出版物
序列 / 3D
常见问题解答
反馈墙 (0)

R&D 技术数据

更多信息，请参阅手册，或联系我们的技术支持: tech@eiaab.com.

基因位点

Cytogenetic band: 17q23.3-q24.1 by HGNC 17q23.3-q24.1 by Entrez Gene 17q23.3-q24.1 by Ensembl

SMURF2 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

通用注释

亚单元:

Interacts (via WW domains) with SMAD1. Interacts (via WW domains) with SMAD2 (via PY-motif). Interacts (via WW domains) with SMAD3 (via PY-motif). Interacts with SMAD6. Interacts with SMAD7 (via PY-motif) and TGFBR1; SMAD7 recruits SMURF2 to the TGF-beta receptor and regulates its degradation. Does not interact with SMAD4; SMAD4 lacks a PY-motif. Interacts with AIMP1. Interacts with STAMBP and RNF11. Interacts with NDFIP1 and NDFIP2 (Probable); this interaction activates the E3 ubiquitin-protein ligase.

功能:

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.

亚细胞位置:

Nucleus Cytoplasm Cell membrane Membrane raft Cytoplasmic in the presence of SMAD7. Colocalizes with CAV1, SMAD7 and TGF-beta receptor in membrane rafts.

数据库链接

UniGene:

Hs.515011

SMR:

Q9HAU4

STRING:

9606.ENSP00000262435

KEGG:

hsa:64750

MIM:

605532

Pfam:

PF00168

Uniprot:

Q9HAU4

相关产品

Mouse Smurf2 ELISA Kit

Mouse Smurf2 CLIA Kit

Mouse Smurf2 Protein

Mouse Smurf2 Polyclonal Antibody

Mouse Smurf2 Monoclonal Antibody

序列长度:

748

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

550:

600:

650:

700:

748:

HQIDACTNNL | PKAHTCFNRI | DIPPYESYEK | LYEKLLTAIE | ETCGFAVE

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"Autoinhibition of the HECT-type ubiquitin ligase Smurf2 through its C2 domain."

(PMID: 17719543)

[2].

"An expanded WW domain recognition motif revealed by the interaction between Smad7 and the E3 ubiquitin ligase Smurf2."

(PMID: 16641086)

[3].

"Regulation of Smurf2 ubiquitin ligase activity by anchoring the E2 to the HECT domain."

(PMID: 16061177)

[4].

"The RING-H2 protein RNF11 is overexpressed in breast cancer and is a target of Smurf2 E3 ligase."

(PMID: 14562029)

[5].

"Regulation of Smad degradation and activity by Smurf2, an E3 ubiquitin ligase."

(PMID: 11158580)

[6].

"Smurf2 is a ubiquitin E3 ligase mediating proteasome-dependent degradation of Smad2 in transforming growth factor-beta signaling."

(PMID: 11016919)

[7].

"SCFFBXL1? regulates BMP signalling by directing the degradation of HECT-type ubiquitin ligase Smurf1."

(PMID: 21572392)

[8].

"Pin1 down-regulates transforming growth factor-beta (TGF-beta) signaling by inducing degradation of Smad proteins."

(PMID: 19122240)

[9].

"Smurf2 participates in human trophoblast cell invasion by inhibiting TGF-beta type I receptor."

(PMID: 19255252)

[10].

"Role of SARA (SMAD anchor for receptor activation) in maintenance of epithelial cell phenotype."

(PMID: 19620243)

[1].

人E3泛素蛋白连接酶Smurf2(SMURF2)重组蛋白是否是无菌的？

蛋白试剂瓶和蛋白保存液是经过高压灭菌的，但也不能保证蛋白是完全无菌的。如果要求蛋白是无菌的，可以用0.2微米的滤器对蛋白进行过滤。

[2].

人E3泛素蛋白连接酶Smurf2(SMURF2)重组蛋白的保存缓冲液是什么？

纯化后的蛋白保存在PBS（58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.4）里，并往里面加入500mM咪唑和10%甘油。

[3].

怎样确定人E3泛素蛋白连接酶Smurf2(SMURF2)重组蛋白的浓度？

蛋白浓度的确定没有一个统一的标准，这主要取决于蛋白的氨基酸序列。伊艾博是根据不同测试的组合来测定蛋白浓度。考马斯亮蓝法、BCA法、氨基酸序列和氨基酸全序列分析法等都用来测定蛋白浓度。

[4].

人E3泛素蛋白连接酶Smurf2(SMURF2)重组蛋白蛋白保存条件是怎样的？

蛋白应保存在 -20℃或 -80℃条件下，为了避免反复冻融，可以将蛋白分装成小份保存。

[5].

人E3泛素蛋白连接酶Smurf2(SMURF2)重组蛋白是否可以用于活体实验？

重组蛋白没有用于任何的活体实验，因此蛋白的活性和半衰期是不确定的。

[6].

人E3泛素蛋白连接酶Smurf2(SMURF2)重组蛋白的保质期是多久？

在适当的保存条件下，从购买之日起蛋白可以稳定保存6-12个月。适当的保存条件是:蛋白保存在-20°C o或 -80℃，保证蛋白的保存浓度高于0.1mg/ml，限制蛋白反复冻融的次数。我们公司常规的质量检测保证所有产品在销售时都有可接受的生物活性。但是我们不能控制终端用户蛋白的保存条件。如果产品在有效期内出现问题，请联系我们的技术支持。

[7].

你们蛋白和抗体的报价是怎么样的？

我们将根据你需要的蛋白和抗体的大小进行报价。

[8].

人E3泛素蛋白连接酶Smurf2(SMURF2)重组蛋白是否能够提供蛋白片段？

我们现有的人的蛋白的序列可以有很多。你可以选择你感兴趣的靶向部分，我们将会按您的需求提供蛋白和抗体。

[9].

人E3泛素蛋白连接酶Smurf2(SMURF2)重组蛋白的货期或发货时间一般是多长？

具体指标的货期需要确定。最快一周，最长可能一个月。

反馈墙

评论数 : 0

所有用户