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Atf2 (基因名), Cyclic AMP-dependent transcription factor ATF-2 (蛋白名), atf2_mouse.

产品名称:

Mouse Atf2/ Cyclic AMP-dependent transcription factor ATF-2 ELISA Kit
环AMP依赖性转录因子ATF-2

货号:

E8848m

商标:

EIAab^®

监管等级:

RUO

别名:

Activating transcription factor 2, MXBP protein, cAMP response element-binding protein CRE-BP1, cAMP-dependent transcription factor ATF-2

检测方法:

ELISA

实验类型:

Sandwich

检测范围:

0.312-20ng/mL

灵敏度:

0.181ng/mL

特异性:

Natural and recombinant mouse Cyclic AMP-dependent transcription factor ATF-2

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

实验步骤:

研究领域:

Immunology

规格 & 价格:

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产品说明书

说明书:

MSDS:

在线询价

数据
文献
序列 / 3D
常见问题解答
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精密度

批内差：已知浓度的3个样本在一个板子内重复检测20次，以评估批内精密度。
批内 CV： ≤6.8%
批间差：已知浓度的3个样本在不同的板子上重复测定5次，以评估测定批间精密度。
批间 CV： ≤9.4%

回收率

回收率：低、中和高浓度的分析物被掺入到血清或者血浆样本中，进行回收实验测定。

Sample Type	Average(%)	Recovery Range(%)
Serum	87	81-93
Plasma	89	83-95

线性

线性：给定样本通过梯度稀释，每次稀释的测量值与理论值的比值。

Sample	1：2	1:4	1:8	1:16
serum(n=5)	104-116%	83-95%	108-118%	102-113%
EDTA plasma(n=5)	87-99%	95-107%	92-101%	98-108%
heparin plasma(n=5)	111-111%	107-119%	113-113%	97-105%

通用注释

亚单元:

Binds DNA as a dimer and can form a homodimer in the absence of DNA. Can form a heterodimer with JUN. Heterodimerization is essential for its transcriptional activity. Interacts with SMAD3 and SMAD4. Interacts with the HK1/VDAC1 complex. Interacts with NBN, MRE11, XPO1, KAT5 and CUL3 (By similarity). Binds through its N-terminal region to UTF1 which acts as a coactivator of ATF2 transcriptional activity.

功能:

Transcriptional activator which regulates the transcription of various genes, including those involved in anti-apoptosis, cell growth, and DNA damage response. Dependent on its binding partner, binds to CRE (cAMP response element) consensus sequences (5'-TGACGTCA-3') or to AP-1 (activator protein 1) consensus sequences (5'-TGACTCA-3'). In the nucleus, contributes to global transcription and the DNA damage response, in addition to specific transcriptional activities that are related to cell development, proliferation and death. In the cytoplasm, interacts with and perturbs HK1- and VDAC1-containing complexes at the mitochondrial outer membrane, thereby impairing mitochondrial membrane potential, inducing mitochondrial leakage and promoting cell death. The phosphorylated form (mediated by ATM) plays a role in the DNA damage response and is involved in the ionizing radiation (IR)-induced S phase checkpoint control and in the recruitment of the MRN complex into the IR-induced foci (IRIF). Exhibits histone acetyltransferase (HAT) activity which specifically acetylates histones H2B and H4 in vitro. In concert with CUL3 and RBX1, promotes the degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. Can elicit oncogenic or tumor suppressor activities depending on the tissue or cell type.

亚细胞位置:

Nucleus Cytoplasm Mitochondrion outer membrane Shuttles between the cytoplasm and the nucleus and heterodimerization with JUN is essential for the nuclear localization. Localization to the cytoplasm is observed under conditions of cellular stress and in disease states. Localizes at the mitochondrial outer membrane in response to genotoxic stress. Phosphorylation at Thr-34 is required for its nuclear localization and negatively regulates its mitochondrial localization. Colocalizes with the MRN complex in the IR-induced foci (IRIF) (By similarity).

数据库链接

UniGene:

Mm.209903

SMR:

P16951

STRING:

10090.ENSMUSP00000058521

KEGG:

mmu:11909

Pfam:

PF00170

Uniprot:

P16951

相关产品

Human ATF2 ELISA Kit

Rat Atf2 ELISA Kit

Chicken ATF2 ELISA Kit

Human ATF2 CLIA Kit

Rat Atf2 CLIA Kit

序列长度:

487

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

250:

300:

350:

400:

450:

487:

AEAVATSVLT | QMADQSTEPA | LSQIVMAPPS | QAQPSGS

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

小鼠环AMP依赖性转录因子ATF-2(Atf2)ELISA试剂盒可以做多少个样本？

小鼠环AMP依赖性转录因子ATF-2(Atf2)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

小鼠环AMP依赖性转录因子ATF-2(Atf2)ELISA试剂盒使用视频？

小鼠环AMP依赖性转录因子ATF-2(Atf2)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

小鼠环AMP依赖性转录因子ATF-2(Atf2)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的小鼠环AMP依赖性转录因子ATF-2(Atf2)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

小鼠环AMP依赖性转录因子ATF-2(Atf2)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

小鼠环AMP依赖性转录因子ATF-2(Atf2)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析小鼠环AMP依赖性转录因子ATF-2(Atf2)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

小鼠环AMP依赖性转录因子ATF-2(Atf2)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集小鼠环AMP依赖性转录因子ATF-2(Atf2)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

小鼠环AMP依赖性转录因子ATF-2(Atf2)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

小鼠环AMP依赖性转录因子ATF-2(Atf2)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

小鼠环AMP依赖性转录因子ATF-2(Atf2)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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Mouse Atf2/ Cyclic AMP-dependent transcription factor ATF-2 ELISA Kit 环AMP依赖性转录因子ATF-2

E8848m

Activating transcription factor 2, MXBP protein, cAMP response element-binding protein CRE-BP1, cAMP-dependent transcription factor ATF-2

Mouse Atf2/ Cyclic AMP-dependent transcription factor ATF-2 ELISA Kit
环AMP依赖性转录因子ATF-2