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CEBPB (基因名), CCAAT/enhancer-binding protein beta (蛋白名), cebpb_bovin.

产品名称:

Bovine CEBPB/ CCAAT/enhancer-binding protein beta ELISA Kit
CCAAT / enhancer-binding蛋白β

货号:

E0713b

商标:

EIAab^®

监管等级:

RUO

别名:

C/EBP beta

检测方法:

ELISA

实验类型:

Sandwich

检测范围:

0.312-20ng/ml

灵敏度:

0.084ng/ml

特异性:

Natural and recombinant bovine CCAAT/enhancer-binding protein beta

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

实验步骤:

研究领域:

Cancer

规格 & 价格:

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产品说明书

说明书:

MSDS:

在线询价

数据
文献
序列 / 3D
常见问题解答
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通用注释

亚单元:

Binds DNA as a homodimer and as a heterodimer. Interacts with ATF4. Binds DNA as a heterodimer with ATF4. Interacts with MYB; within the complex, MYB and CEBPB bind to different promoter regions. Can form stable heterodimers with CEBPA, CEBPD and CEBPG. Interacts with TRIM28 and PTGES2. Interacts with PRDM16. Interacts with CCDC85B. Forms a complex with THOC5. Interacts with ZNF638; this interaction increases transcriptional activation. Interacts with CIDEA and CIDEC; these interactions increase transcriptional activation of a subset of CEBPB downstream target genes. Interacts with DDIT3/CHOP.Interacts with EP300; recruits EP300 to chromatin. Interacts with RORA; the interaction disrupts interaction with EP300. Interacts (not methylated) with MED23, MED26, SMARCA2, SMARCB1 and SMARCC1. Interacts with KAT2A and KAT2B (By similarity). Interacts with ATF5; EP300 is required for ATF5 and CEBPB interaction and DNA binding.

功能:

Important transcription factor regulating the expression of genes involved in immune and inflammatory responses. Plays also a significant role in adipogenesis, as well as in the gluconeogenic pathway, liver regeneration, and hematopoiesis. The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'. Its functional capacity is governed by protein interactions and post-translational protein modifications. During early embryogenesis, plays essential and redundant functions with CEBPA. Has a promitotic effect on many cell types such as hepatocytes and adipocytes but has an antiproliferative effect on T-cells by repressing MYC expression, facilitating differentiation along the T-helper 2 lineage. Binds to regulatory regions of several acute-phase and cytokines genes and plays a role in the regulation of acute-phase reaction and inflammation. Plays also a role in intracellular bacteria killing. During adipogenesis, is rapidly expressed and, after activation by phosphorylation, induces CEBPA and PPARG, which turn on the series of adipocyte genes that give rise to the adipocyte phenotype. The delayed transactivation of the CEBPA and PPARG genes by CEBPB appears necessary to allow mitotic clonal expansion and thereby progression of terminal differentiation. Essential for female reproduction because of a critical role in ovarian follicle development. Restricts osteoclastogenesis.

亚细胞位置:

Nucleus Cytoplasm Translocates to the nucleus when phosphorylated at Ser-288. In T-cells when sumoylated drawn to pericentric heterochromatin thereby allowing proliferation (By similarity).

数据库链接

UniGene:

Bt.64509

SMR:

O02755

KEGG:

bta:338319

Pfam:

PF07716

Uniprot:

O02755

相关产品

Human CEBPB ELISA Kit

Mouse Cebpb ELISA Kit

Rat Cebpb ELISA Kit

Chicken CEBPB ELISA Kit

Human CEBPB CLIA Kit

序列长度:

348

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

250:

300:

348:

ETQHKVLELT | GENERLQKKV | EQLSREVSTL | RNLFKTLPEP | LLASSGHC

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

牛CCAAT / enhancer-binding蛋白β(CEBPB)ELISA试剂盒可以做多少个样本？

牛CCAAT / enhancer-binding蛋白β(CEBPB)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

牛CCAAT / enhancer-binding蛋白β(CEBPB)ELISA试剂盒使用视频？

牛CCAAT / enhancer-binding蛋白β(CEBPB)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

牛CCAAT / enhancer-binding蛋白β(CEBPB)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的牛CCAAT / enhancer-binding蛋白β(CEBPB)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

牛CCAAT / enhancer-binding蛋白β(CEBPB)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

牛CCAAT / enhancer-binding蛋白β(CEBPB)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析牛CCAAT / enhancer-binding蛋白β(CEBPB)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

牛CCAAT / enhancer-binding蛋白β(CEBPB)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集牛CCAAT / enhancer-binding蛋白β(CEBPB)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

牛CCAAT / enhancer-binding蛋白β(CEBPB)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

牛CCAAT / enhancer-binding蛋白β(CEBPB)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

牛CCAAT / enhancer-binding蛋白β(CEBPB)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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Bovine CEBPB/ CCAAT/enhancer-binding protein beta ELISA Kit CCAAT / enhancer-binding蛋白β

E0713b

C/EBP beta

Bovine CEBPB/ CCAAT/enhancer-binding protein beta ELISA Kit
CCAAT / enhancer-binding蛋白β