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FGF1 (基因名), Fibroblast growth factor 1 (蛋白名), fgf1_bovin.

产品名称:

Bovine FGF1/ Fibroblast growth factor 1 ELISA Kit
肝素结合生长因子1

货号:

E0032b

商标:

EIAab^®

监管等级:

RUO

别名:

Acidic eye-derived growth factor II, Acidic fibroblast growth factor, Endothelial cell growth factor, Heparin-binding growth factor 1, Prostatropin, EDGF II, aFGF, ECGF, HBGF-1, FGF-1, AFGF, FGFA, HBGF-1

检测方法:

ELISA

实验类型:

Sandwich

检测范围:

0.156-10ng/mL

灵敏度:

0.053ng/mL

特异性:

Natural and recombinant bovine Fibroblast growth factor 1

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

实验步骤:

研究领域:

规格 & 价格:

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产品说明书

说明书:

MSDS:

在线询价

数据
文献
序列 / 3D
常见问题解答
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通用注释

亚单元:

Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Found in a complex with FGFBP1, FGF1 and FGF2. Interacts with FGFBP1. Part of a Cu(2+)-dependent multiprotein aggregate containing FGF1, S100A13 and SYT1. Interacts with S100A13. Interacts with FGFBP1 (By similarity). Interacts with LRRC59 (By similarity). Interacts with CSNKA, CSNKB and FIBP (By similarity). While binding with LRRC59, CSNKA and FIBP seem mutually exclusive, CSNKB and FIBP may cooperatively interact with FGF1 (By similarity). Interacts with SYT1 (PubMed:9712834). Forms a ternary complex with FGFR1 and ITGAV:ITGB3 and induces the recruitment of PTPN11 to the complex.

功能:

Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Acts as a ligand for FGFR1 and integrins. Binds to FGFR1 in the presence of heparin leading to FGFR1 dimerization and activation via sequential autophosphorylation on tyrosine residues which act as docking sites for interacting proteins, leading to the activation of several signaling cascades. Binds to integrin ITGAV:ITGB3. Its binding to integrin, subsequent ternary complex formation with integrin and FGFR1, and the recruitment of PTPN11 to the complex are essential for FGF1 signaling. Induces the phosphorylation and activation of FGFR1, FRS2, MAPK3/ERK1, MAPK1/ERK2 and AKT1. Can induce angiogenesis.

亚细胞位置:

Secreted Cytoplasm Cytoplasm Cell cortex Cytoplasm Cytosol Nucleus Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1. Binding of exogenous FGF1 to FGFR facilitates endocytosis followed by translocation of FGF1 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as LRRC59 (By similarity).

数据库链接

UniGene:

Bt.5038

SMR:

P03968

STRING:

9913.ENSBTAP00000040651

KEGG:

bta:281160

Pfam:

PF00167

Uniprot:

P03968

相关产品

Human FGF1 ELISA Kit

Mouse Fgf1 ELISA Kit

Rat Fgf1 ELISA Kit

Chicken FGF1 ELISA Kit

Pig FGF1 ELISA Kit

序列长度:

155

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

155:

PVSSD

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

牛肝素结合生长因子1(FGF1)ELISA试剂盒可以做多少个样本？

牛肝素结合生长因子1(FGF1)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

牛肝素结合生长因子1(FGF1)ELISA试剂盒使用视频？

牛肝素结合生长因子1(FGF1)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

牛肝素结合生长因子1(FGF1)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的牛肝素结合生长因子1(FGF1)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

牛肝素结合生长因子1(FGF1)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

牛肝素结合生长因子1(FGF1)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析牛肝素结合生长因子1(FGF1)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

牛肝素结合生长因子1(FGF1)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集牛肝素结合生长因子1(FGF1)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

牛肝素结合生长因子1(FGF1)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

牛肝素结合生长因子1(FGF1)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

牛肝素结合生长因子1(FGF1)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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Bovine FGF1/ Fibroblast growth factor 1 ELISA Kit 肝素结合生长因子1

E0032b

Acidic eye-derived growth factor II, Acidic fibroblast growth factor, Endothelial cell growth factor, Heparin-binding growth factor 1, Prostatropin, EDGF II, aFGF, ECGF, HBGF-1, FGF-1, AFGF, FGFA, HBGF-1

Bovine FGF1/ Fibroblast growth factor 1 ELISA Kit
肝素结合生长因子1