首页 > 酶联免疫试剂盒 > Human JMJD6 ELISA Kit

JMJD6 (基因名), Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6 (蛋白名), jmjd6_human.

产品名称:

Human JMJD6/ Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6 ELISA Kit
双功能精氨酸去甲基酶和赖氨酸羟化酶JMJD6

货号:

E1916h

商标:

EIAab^®

监管等级:

RUO

别名:

Histone arginine demethylase JMJD6, JmjC domain-containing protein 6, Jumonji domain-containing protein 6, Lysyl-hydroxylase JMJD6, Peptide-lysine 5-dioxygenase JMJD6, Phosphatidylserine receptor, Protein PTDSR, KIAA0585, PSR, PTDSR

检测方法:

ELISA

实验类型:

Sandwich

检测范围:

0.625-40ng/mL

灵敏度:

0.34ng/mL

特异性:

Natural and recombinant human Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

实验步骤:

研究领域:

Immunology

规格 & 价格:

关注

Human JMJD6 ELISA Kit

邮箱 *

消息 *

Please 登录.

产品说明书

说明书:

MSDS:

在线询价

数据
文献
序列 / 3D
常见问题解答
反馈墙 (0)

通用注释

亚单元:

Homooligomerizes; requires lysyl-hydroxylase activity (PubMed:22189873, PubMed:24360279). Interacts with LUC7L2, LUC7L3 and U2AF2/U2AF65 (PubMed:19574390). Interacts with LIAT1 (By similarity). Interacts with CDK9 and CCNT1; the interaction is direct with CDK9 and associates the P-TEFb complex when active (PubMed:24360279). Interacts (via JmjC and N-terminal domains) with BRD4 (via NET domain); the interaction is stronger in presence of ssRNA and recruits JMJD6 on distal enhancers (PubMed:21555454, PubMed:24360279, PubMed:29176719).

功能:

Dioxygenase that can both act as a arginine demethylase and a lysyl-hydroxylase (PubMed:24498420, PubMed:17947579, PubMed:20684070, PubMed:21060799, PubMed:22189873). Acts as a lysyl-hydroxylase that catalyzes 5-hydroxylation on specific lysine residues of target proteins such as U2AF2/U2AF65 and LUC7L2. Regulates RNA splicing by mediating 5-hydroxylation of U2AF2/U2AF65, affecting the pre-mRNA splicing activity of U2AF2/U2AF65 (PubMed:19574390). Hydroxylates its own N-terminus, which is required for homooligomerization (PubMed:22189873). In addition to peptidyl-lysine 5-dioxygenase activity, may act as an RNA hydroxylase, as suggested by its ability to bind single strand RNA (PubMed:20679243, PubMed:29176719). Also acts as an arginine demethylase which preferentially demethylates asymmetric dimethylation (PubMed:17947579, PubMed:24498420, PubMed:24360279). Demethylates histone H3 at 'Arg-2' (H3R2me) and histone H4 at 'Arg-3' (H4R3me), including mono-, symmetric di- and asymmetric dimethylated forms, thereby playing a role in histone code (PubMed:17947579, PubMed:24360279). However, histone arginine demethylation may not constitute the primary activity in vivo (PubMed:17947579, PubMed:21060799, PubMed:22189873). In collaboration with BRD4, interacts with the positive transcription elongation factor b (P-TEFb) complex in its active form to regulate polymerase II promoter-proximal pause release for transcriptional activation of a large cohort of genes. On distal enhancers, so called anti-pause enhancers, demethylates both histone H4R3me2 and the methyl cap of 7SKsnRNA leading to the dismissal of the 7SKsnRNA:HEXIM1 inhibitor complex. After removal of repressive marks, the complex BRD4:JMJD6 attract and retain the P-TEFb complex on chromatin, leading to its activation, promoter-proximal polymerase II pause release, and transcriptional activation (PubMed:24360279). Demethylates other arginine methylated-proteins such as ESR1 (PubMed:24498420). Has no histone lysine demethylase activity (PubMed:21060799). Required for differentiation of multiple organs during embryogenesis. Acts as a key regulator of hematopoietic differentiation: required for angiogenic sprouting by regulating the pre-mRNA splicing activity of U2AF2/U2AF65 (By similarity). Seems to be necessary for the regulation of macrophage cytokine responses (PubMed:15622002).

亚细胞位置:

Nucleus Nucleoplasm Nucleus Nucleolus Cytoplasm Mainly found throughout the nucleoplasm outside of regions containing heterochromatic DNA, with some localization in nucleolus. During mitosis, excluded from the nucleus and reappears in the telophase of the cell cycle.

数据库链接

UniGene:

Hs.514505

SMR:

Q6NYC1

STRING:

9606.ENSP00000394085

KEGG:

hsa:23210

MIM:

604914

Pfam:

PF02373

Uniprot:

Q6NYC1

相关产品

Mouse Jmjd6 ELISA Kit

Rat Jmjd6 ELISA Kit

Bovine JMJD6 ELISA Kit

Chicken JMJD6 ELISA Kit

Mouse Jmjd6 CLIA Kit

序列长度:

403

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

250:

300:

350:

400:

409:

VSKERSSSR

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

人双功能精氨酸去甲基酶和赖氨酸羟化酶JMJD6(JMJD6)ELISA试剂盒可以做多少个样本？

人双功能精氨酸去甲基酶和赖氨酸羟化酶JMJD6(JMJD6)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

人双功能精氨酸去甲基酶和赖氨酸羟化酶JMJD6(JMJD6)ELISA试剂盒使用视频？

人双功能精氨酸去甲基酶和赖氨酸羟化酶JMJD6(JMJD6)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

人双功能精氨酸去甲基酶和赖氨酸羟化酶JMJD6(JMJD6)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的人双功能精氨酸去甲基酶和赖氨酸羟化酶JMJD6(JMJD6)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

人双功能精氨酸去甲基酶和赖氨酸羟化酶JMJD6(JMJD6)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

人双功能精氨酸去甲基酶和赖氨酸羟化酶JMJD6(JMJD6)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析人双功能精氨酸去甲基酶和赖氨酸羟化酶JMJD6(JMJD6)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

人双功能精氨酸去甲基酶和赖氨酸羟化酶JMJD6(JMJD6)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集人双功能精氨酸去甲基酶和赖氨酸羟化酶JMJD6(JMJD6)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

人双功能精氨酸去甲基酶和赖氨酸羟化酶JMJD6(JMJD6)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

人双功能精氨酸去甲基酶和赖氨酸羟化酶JMJD6(JMJD6)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

人双功能精氨酸去甲基酶和赖氨酸羟化酶JMJD6(JMJD6)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

反馈墙

评论数 : 0

所有用户

默认排序

Human JMJD6/ Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6 ELISA Kit 双功能精氨酸去甲基酶和赖氨酸羟化酶JMJD6

E1916h

Histone arginine demethylase JMJD6, JmjC domain-containing protein 6, Jumonji domain-containing protein 6, Lysyl-hydroxylase JMJD6, Peptide-lysine 5-dioxygenase JMJD6, Phosphatidylserine receptor, Protein PTDSR, KIAA0585, PSR, PTDSR

Human JMJD6/ Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6 ELISA Kit
双功能精氨酸去甲基酶和赖氨酸羟化酶JMJD6